Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Total Phenolic Compound, Total Flavonoid Compound And Anti-Inflammatory Inhibitory Effects of Psidium Guajava Leaf Extract

구아바 잎 추출물의 총 폴리페놀과 플라보노이드 함량 및 항 염증 억제 효과

  • Lee, Jeong-Seon (Department of Cosmetology Science, Nambu University) ;
  • Kim, Chun-Dug (Department of Cosmetology Science, Nambu University)
  • Received : 2017.12.15
  • Accepted : 2018.03.22
  • Published : 2018.03.30
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Abstract

It was intended to check a possibility of activity of Psidium guajava leaf extract as anti-oxidant and anti-inflammatory material. Total polyphenol and total flavonoid content of Psidium guajava leaf extract was checked. Cream having inhibitory effect on inflammation through toxicity and NO production inhibition in RAW 264.7 cell was manufactured, and skin safety was evaluated. It was confirmen that total polyphenol and flavonoid content of Psidium guajava leaf extract was 126.4 mg/g and 223.17 mg/g respectively, which was high content. According to the results of checking toxicity through cell viability in RAW 264.7 cell, cytotoxicity was not shown. And NO production indicating inflammatory disease was inhibited concentration-dependently. According to the results of carrying out single patch test after manufacturing the cream containing the Psidium guajava leaf extract, skin irritation did not occur for 24 h to put patch on skin or for 24 h after removing the patch. Putting these results together, it was verified that there was possibility of application as raw materials for cosmetics, which would have anti-oxidant activity owing to the high polyphenol and flavonoid content of Psidium guajava leaf extract and anti-inflammatory material through NO production inhibition.

구아바 잎 추출물의 항산화 활성과 항염증 소재로서의 활용 가능성을 확인하고자 하였다. 구아바 잎 추출물의 총 폴리페놀 함량, 총 플라보노이드함량, RAW 264.7 세포에 대한 세포 독성 및 NO 생성 억제를 통한 항염증 효과, 피부에 대한 안전성 평가를 실시하였다. 본 실험 결과, 총 폴리페놀과 플라보노이드의 함량이 각 126.4 mg/g, 223.17 mg/g으로 높은 함량을 확인하였다. 대식세포인 264.7세포에 대한 세포 독성이 나타나지 않았으며 NO 생성을 농도 의존적으로 억제되는 것을 확인하였다. 구아바 잎 추출물을 함유한 크림을 제조하여 1차 첩포 테스트를 실시하여 피부 안전성을 확인한 결과 첩포 24 시간 후와 첩포 제거 24 시간 후에도 피부에 대한 자극은 확인되지 않았다. 이러한 결과를 종합해 볼 때 높은 총 폴리페놀과 플라보노이드의 함량으로 인한 항산화 활성이 우수하고, 세포에 대한 피부 독성이 적고 NO 생성 억제 효과가 확인됨에 따라 항산화 및 항염증 효과를 가진 소재로서의 활용 가능성을 확인하였다.

Keywords

References

  1. Maines MD, "The oxygenase system: a regular of second messenger gases," Annu. Rev. Pharmacol Toxicol., Vol. 37, pp. 517-554, (1997). https://doi.org/10.1146/annurev.pharmtox.37.1.517
  2. Zedler S, Faist E, "The impact of endogenous triggers on trauma associated inflammation," Curr Opin Crit Care. Vol. 12., pp. 595-601, (2006). https://doi.org/10.1097/MCC.0b013e3280106806
  3. Guha M, Mackman N, “The phosphatidylinositol 3-kinase-Akt pathway limits lipopolysaccharide activation of signaling pathways and expression of inflammatory mediators in human monocytic cells,” J. Biol chem., Vol. 277, No. 35, pp. 32124-32132, (2002). https://doi.org/10.1074/jbc.M203298200
  4. Nathan PA, “Review of ergonomic studies of carpal tunnel syndrome,” AM. J. Ind. Med., Vol. 2, No. 6, pp. 895-897, (1992).
  5. Shew RL, Papka RE, McNeill DL, Yee JA. "NADPH-diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction," Peptides. Vol. 14, pp. 637, (1993). https://doi.org/10.1016/0196-9781(93)90157-C
  6. Kwqagkmata H, Ochiai H, Mantani N, Terasawa K, "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells," a murinemacrophage cell line. Am. J. Chin. Med., Vol. 28, pp. 217, (2000). https://doi.org/10.1142/S0192415X0000026X
  7. Iyengar R, Sutehr DJ, Marletta MA. "Macrophage synthesis of nitrite, nitrate and N-nitrosamines : Precursors and cytotoxic activities of pomegranate (Punica granatum) leaves," Food and Chem. Toxico., Vol. 55, pp. 470-475, (1987).
  8. Snyder SH, Bredt DS, "Biological roles of nitric oxide," Sci. Am., Vol. 266, 68-77, (1992).
  9. Weller R, "Nitric oxide-a newly discovered chemical transmitter in human skin," Br. J. Dermatol., Vol. 137., pp. 665, (1997). https://doi.org/10.1111/j.1365-2133.1997.tb01099.x
  10. Brown GC, "NO says yes to mitochondria," Cell Biol., Vol. 299, 838-839, (2003).
  11. You HJ, Hong SU, “A Case of Urticarial Drug Eruption Assumed to be Caused by non-steroidal anti-inflammatory drugs (NSAIDs),” J Korean Med. Ophthalmol Otolaryngol Dermatol., Vol. 20, No. 1, pp. 256-264, (2007).
  12. You SH, Moon JS, “A Study on Anti-oxidative, Anti-inflammatory and Melanin Inhibitory Effects of Chrysanthemum Sibiricum Extract,” J. of Korean Oil Chemists's Soc., Vol. 33, No. 4, pp. 762-770, (2016). https://doi.org/10.12925/jkocs.2016.33.4.762
  13. You SH, Pyo YH, "Antioxidant and Anti-inflammatory Activities of Ethanol Extracts from Wheat Sprout," J Invest Cosmetol., Vol. 11, pp. 231-238, (2015). https://doi.org/10.15810/jic.2015.11.3.007
  14. Kim MJ, Lee SP, Choi JH, Kwon SH, Kim HD, Bang MH, Yang SA. "Characteristics of fermented dropwort extract and vinegar using fermented dropwort extract and its protective effects on oxidative damage in rat glioma C6 cells," Kor. J. Food Sci. Technol., Vol. 45, pp. 350-355, (2013). https://doi.org/10.9721/KJFST.2013.45.3.350
  15. Cho YJ, "Study on the tyrosinase activity inhibition and free radical scavenging activity of natural materials," SookMyung University Msater's thesis, (2004).
  16. Kim JH, "The Study on the Physiological Activities of Liriope platyphylla Flower Extract," Daegu Haany University Msater's thesis, (2013).
  17. Han HS, Kim SY, Lim DJ, Hwang WK. Development of Whitening Cosmetic Ingredients from Cudrania tricuspidata Stem Extract," Asian J Beauty Cosmetol. Vol. 14, No. 3, pp. 317-328, (2016). https://doi.org/10.20402/ajbc.2016.0063
  18. Choi MH, "Whitening effects screening of Chenopodium album var. centrorubrum extracts as a functional cosmetics material," WonKwang University Doctorate thesis, (2014).
  19. Kim EH, “A Study of Whitening Cosmetics from natural products,” Asian J Beauty Cosmetol., Vol. 4, No. 2, pp. 195-203, (2006).
  20. Lee EJ. Hwang WK, "Research Paper : The Development of Anti-wrinkle Cosmeceutical Ingredients from Glehniae Radix cum Rhizoma," J. Kor. Soc. Cosm. Vol. 17, No. 1, pp. 127-133, (2011).
  21. Lee MY, Kim BA, Yang JC, “Effects of Extracts Derived from Red Ginseng Residue on Antioxidant Activity and Elastase Inhibition,” J. of Korean Oil Chemists's Soc., Vol. 33, No. 4, pp. 658-666, (2016). https://doi.org/10.12925/jkocs.2016.33.4.658
  22. Kim MJ, You MJ, “Inhibition of Melanogenesis and Anti-UV properties Reynoutria elliptica,” Asian J Beauty Cosmetol., Vol. 10, No. 4, pp. 961-968, (2012).
  23. Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi KP, Park WY, Lee HY. "Skin-Whitening and UV-Protective Effects of Angelica gigas Nakai Extracts on Ultra High Pressure Extraction Process," KJMCS. Vol. 16, No. 4, pp. 255-260, (2008).
  24. Begum S, Hassa SI, Siddiqui BS, Shaheen F, Ghayur MN, Gilani AH, "Triterpenoids from the leaves of Psidium guajava," Phytochemistry., Vol. 61, pp. 399-403, (2002). https://doi.org/10.1016/S0031-9422(02)00190-5
  25. Mercadante AZ, Steck A, Pfander H. "Carotenoids from guava (Psidium guajava L.) : isolation and structure elucidation," J Agric Food Chem., Vol. 47, pp. 145-151, (1999). https://doi.org/10.1021/jf980405r
  26. Miean KH, Mohamed S, "Flavonoid (myricetin, quercetin kaempferol, luteolin, and apigenin) content of edible tropical plants," J Agric Food Chem., Vol. 49, pp. 3106-3112, (2001). https://doi.org/10.1021/jf000892m
  27. Meckes M, Calzada F, Tortoriello J, Gonzalez JL, Martinez M, "Terpenoids isolated from Psidium guajava hexane extract with depressant activity on central nervous system," Phytother Res., Vol. 10, pp. 600-603, (1996). https://doi.org/10.1002/(SICI)1099-1573(199611)10:7<600::AID-PTR918>3.0.CO;2-7
  28. Lozoya X, Meckes M, Abou-Zaid M, Tortoriello J, Nozzolillo J, Arnason JT. "Quercetin glycosides in Psidium guajava L. leaves and determination of spasmolytic principle," Arch. Med. Res., Vol. 25, pp. 11-15, (1994).
  29. Jaiarj P, Wongkrajang Y, Thongpraditchote S, Peungvicha P, Bunyapraphatsara N, Opartkiattikul N, "Guava leaf extract and topical haemostasis," Phytother Res. Vol. 14, pp. 388-391, (2000). https://doi.org/10.1002/1099-1573(200008)14:5<388::AID-PTR638>3.0.CO;2-K
  30. Tanaka T, Ishida N, Ishimatsu M, Nonaka G, Nishioka I, "Tannins and related compounds. CXVI. Six new complex tannins, guajavins, psidinins and psiguavin from the bark of Psidium guajava L," Chem. Pharm. Bull., Vol. 40, pp. 2092-2098, (1992). https://doi.org/10.1248/cpb.40.2092
  31. Kim MJ, Choi HY, Kim SI, "Quality Characteristics and Antioxidative Activities of Guavapyun Added Korean Guava Fruit Extract," Korean J. Food Cookery sci. Vol. 26, pp. 246-251, (2010).
  32. Jeong SA, "Antioxidant and Antimicrobial Activities of Guajava Leaf (Psidium guajava) depending on Extract Solvent," International University, (2012).
  33. You SH, "Antioxidant Activity and Whitening activity of Psidium guajava leaf extract. J. of Korean Oil Chemists's Soc. Vol. 34, No. 2, pp. 296-304, (2017).
  34. Folin O, Denis W, "On phosphotungsticphosphomolybdic compounds as color reagents," J. Biolchem., Vol. 12, pp. 239-243, (1912).
  35. Moreno MI, Isla MI, Sampietro AR, Vattuone MA, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina," J Ethnopharmacol., Vol. 71, pp. 109-114, (2000). https://doi.org/10.1016/S0378-8741(99)00189-0
  36. Agudelo Maria, Rodriguez Carlos A, Zuluaga Andres F, Vesga Omar, "Guidelines on in vivo tests and in vitro tests of cosmetic products," Korea. food. and drug. adminstration, (2011).
  37. Chen HY, Yen GC, "Antioxidant activity and free radical-scavenging capacity of extracts from guava leaves," Food Chemistry., Vol. 101, pp. 686-694, (2007). https://doi.org/10.1016/j.foodchem.2006.02.047
  38. Choi SY, Lim SH, Kim JS, Ha TY, Kim SR, Kang KS, Hwang IK, "Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants," Korean J. Food Sci. Technol., Vol. 37, pp. 549-556, (2005).
  39. Park BJ, Onjo M, “Anti-oxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf,” Korean J. Plant Res., Vol. 21, No. 5, pp. 408-412, (2008).
  40. Chen HY. Yen GC, "Antioxidant activity and free radical-scavenging capactiy of extracts from guava (Psidium guajava L.) leaves," Food Chem., Vol. 101, pp. 686-694, (2007). https://doi.org/10.1016/j.foodchem.2006.02.047
  41. Tachakittirungrod S, Okonogi S, "Chowwanapoonpahn S. Study on antioxidant activity of oertain plants in Thailand: Mechanism of antioxidant action of guava leaf extract," Food Chem. Vol. 103, No. 2, pp. 381-388, (2007). https://doi.org/10.1016/j.foodchem.2006.07.034
  42. Fransworth NR, Bunyapraphatsara N. "Thia Medicinal Plants Recommended for Primary Health Care in Thailand," Mahidol University. Bangkok, pp. 202-207, (1990).
  43. Kim MH, "Anti-inflammatory effects of psidium guajava leaf extracts and its compounds in RAW 264.7 macrophages," Catholic University Msater's thesis, (2014).