• Life and Agrochemicals
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• s.283
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• pp.42-44
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• 2012

우리나라 파프리카에 발생하는 주요 바이러스는 오이모자이크바이러스(Cucumber mosaic virus, CMV), 고추모틀바이러스(Pepper mottle virus, PepMoV), 잠두위조바이러스2(Broad bean wilt virus2, BBWV2), 고추약한모틀바이러스(Pepper mild mottle virus, PMMoV), 토마토반점위조바이러스(Tomato spotted wilt virus, TSWV) 및 사탕무황화바이러스(Beet western yellows virus, BWYV) 등 6종이다. 생산량 감소 및 고품질 규격품 생산의 가장 큰 제한요인이 되고 있는 바이러스병의 피해를 최소화하기 하기 위하여 바이러스별 병징 및 발생원인에 따른 예방법을 알아본다.

• Research in Plant Disease
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• v.12 no.2
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• pp.148-151
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• 2006

Tomato spotted wilt virus (TSWV) was occurred newly on the 4 ornamental plants of Impatiens balsamina (Balsam), Dahlia variabilis (Dahlia), Callistephus chinensis (Aster) and Zinnia elegans (Indian lilac) at Anyang area in Korea. TSWV produced the typical symptoms of single or double ring spots on the leaves and induced usually necrosis, wilt and/or severe mosaic.

• Korean journal of applied entomology
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• v.63 no.1
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• pp.43-51
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• 2024

Thrips infest hot peppers (Capsicum annuum) cultivating in open fields and give serious economic damages. This study reports their yearly occurrence from transplanting to harvest at three different places in Andong, an intense hot pepper-cultivating area. Two main occurrence peaks were detected in June and September. Two dominant thrips were the flower flowers, Frankliniella occidentalis and F. intonsa, which comprised of over 87% of the total occurring thrips. Other thrips did not follow the occurrence peaks of the two dominant species. Tomato spotted wilt virus (TSWV) was detected from the dominant two species, in which higher viruliferous rate was recorded in F. intonsa. Although the hot peppers were the resistant varieties against TSWV, some of them cultivating in the fields exhibited a characteristic disease symptom infected by the virus over the growing seasons. TSWV was isolated from the viruliferous thrips and assessed in NSs sequences encoded in S RNA segment of the virus. Compared to the known resistance breaking (RB) strains, the TSWV isolated from the viruliferous thrips in Andong did not show any RB mutations.

• Research in Plant Disease
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• v.22 no.3
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• pp.213-216
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• 2016

Tomato spotted wilt virus (TSWV) is one of the most destructive viruses in tomato plant. TSWV-GT from the leaves of tomato plant showing top wilt symptom in 2015 was used to screen the tolerance in tomato cultivars. Among 51 cultivars commercially available in Korea, 'TY Smartsama' and 'Marnolia' showed tolerance to the virus in bioassay. Three cvs. 'Titichal', 'TY Sensq', and 'Venekia' were moderate tolerance.

• Research in Plant Disease
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• v.23 no.4
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• pp.383-387
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• 2017

In 2016, less than 30% of virus-like symptoms such as chlorosis, necrosis and ringspots were observed in Hoya carnosa from commercial greenhouse in Eumseong, Korea. A total of 6 samples from Hoya carnosa were collected both symptomatic and asymptomatic plants and tested for virus infection by RT-PCR of 3 viruses known to infect Hoya spp. including Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Tomato chlorotic spot virus (TCSV). Three symptomatic samples were positives for INSV. Also, it was not the virus detected in three asymptomatic samples. To further confirm the presence of INSV, complete nucleocapsid (N) gene of the virus were amplified and sequenced from two samples. BLAST analysis of the consensus sequence showed that two isolates (INSV-Hy1 and -Hy2) shared nucleotide sequence identities of 99% with each other and 97-99% with other INSV isolates available in the GenbBank. Phylogenetic analysis showed that these isolates closely related to the INSV isolates from ornamental from China. This is the first report of INSV on Hoya carnosa from Korea.

• Korean Journal of Environmental Biology
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• v.39 no.3
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• pp.319-328
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• 2021

Tomato spotted wilt virus (TSWV) is one of the most destructive viruses worldwide, which causes severe damage to economically important crops, such as pepper and tomato. In this study, we examined the molecular and biological characterization of a TSWV isolate (SW-TO2) infecting tomato and compared it to the recently reported isolates from boxthorn, butterbur, and angelica plants. The phylogenetic analysis based on the complete genome sequences confirmed that SW-TO2 was clustered with those of isolates from boxthorn and pepper in Korea with the maximum nucleotide identities ranging from 98% to 99%. We developed the bioassay method for screening TSWV resistance and tested some commercial pepper and tomato cultivars for resistance evaluation of four isolates of TSWV. TSWV resistance was evaluated as TSWV resistance when all the following three conditions were satisfied: first, when symptoms of necrotic spots or no symptoms were present in the inoculated leaves; second, when there were no symptoms in the upper leaves; and third, when the upper leaves were negative as a result of RT-PCR diagnosis.

• Research in Plant Disease
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• v.11 no.2
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• pp.213-216
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• 2005

Tomato spotted wilt virus (TSWV) occurred abruptly with a high incidence rate in 14 vegetable crops in Anyang area, Gyunggido in 2004. TSWV was identified by the symptoms on the indicator plants, immunocaptured reverse transcription-polymerase chain reaction (IC/RT-PCR), virion captured (VC)RT-PCR and RT-PCR using total RNA from the infected plants. Vegetable crops infected with TSWV included table tomato, cherry tomato, red pepper, lettuce, chicory, red leaf chicory, red mustard, dragon tongue, treviso, potato, perilla, sesame, pumpkin, and ssamchoo (hybrid of oriental cabbage and cabbage). The incidence of TSWV in fields ranged from 30 to $100\%$. TSWV usually produced necrosis, wilt and/or severe mosaic with typical single or double ring spots on the leaves. TSWV could be detected in Frankliniella occidentalis collected from the crops in the fields with $90\%$ rate by IC/RT-PCR.

• Research in Plant Disease
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• v.17 no.1
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• pp.59-65
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• 2011

One hundred Capsicum accessions were screened for symptomatic response and resistance to Tomato spotted wilt virus-pb1 (TSWV-pb1). Symptom and its severity rating were checked by visual observation at 9, 12, 14, and 45 days after inoculation, respectively. Enzyme-linked immune-sorbent assay was performed all tested individuals on non-inoculated upper leaves after the third rating to indentify viral infection. Leaf curling was predominant in almost susceptible individuals of each accession. Stem necrosis was most frequent in wild species while yellowing in commercial hybrids and Korean land race cultivars. Ring spot, a typical symptom of TSWV, was rarely detected in some of a few accessions. Different levels of resistance to TSWV-pb1 were observed among the tested accessions. High level of resistance was detected in 4 commercial cultivars of Kpc-35, -36, -57, and -62, and 8 wild species of PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, and PI201238 through seedling test by mechanical inoculation.

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.486-495
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• 2020

We surveyed disease outbreak status that has recently become a problem in organic tomatoes and cucurbit in plastic greenhouse that were grown without spraying pesticides during the plastic greenhouse growing season of 2015 to 2019. It was found that the incidence of leaf mold, tomato spotted wilt virus, and tomato chlorosis virus disease was severe in tomato, and disease incidence of powdery mildew and zucchini yellow mosaic virus were severe in Cucurbit. The disease outbreak was found to be faster and more severe in crops grown in pesticide-free cultivation plastic greenhouses than in plastic greenhouses that are cultivated in general using pesticides. In particular, the occurrence of viral diseases mediated by thrips and aphids was found to be severely damaged. Therefore, in order to produce good organic products, it is important to effectively control pests, and in order to minimize the damage caused by disease, sanitation and physical blocking, and comprehensively utilize organic materials or microorganisms to prevent them.

• Research in Plant Disease
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• v.19 no.4
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• pp.273-280
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• 2013

Tomato spotted wilt virus (TSWV) was occurred at 8 areas including Naju, Suncheon, Younggwang, Youngam, and Shinan in Jeonnam province and the crops of Younggwang were severely damaged by TSWV. The hot pepper (Capsicum annuum), bell pepper (Capsicum annuum v ar. angulosum) and tomato (Solanum lycopersicon) in greenhouse and hot pepper in open field were infected by TSWV. Especially, hot pepper was severely damaged by TSWV infection. The survey data indicated that 1.1-30% in the nursery field at Naju, Suncheon, and Jangheung were infected by TSWV. Plants were infected by TSWV from early June to August. However, TSWV-infected seedlings from nursery fields showed the disease symptoms from May after transplanting. In pepper greenhouses, Frankliniella occidentalis was more dominant insect vector than Frankliniella intonsa. But in open field, the population of insect vector was opposed to greenhouse. In addition, the removal of weeds was able to delay the incidence of TSWV via side-window of greenhouse in Winter. Taken together, the control of weed and insect vector nearby side-window of greenhouse is important to prevent TSWV infection of plants.