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http://dx.doi.org/10.11626/KJEB.2021.39.3.319

Development of a bioassay for screening of resistance to Tomato spotted wilt virus isolate from Korea  

Kwak, Hae-Ryun (Crop Protection Division, National Institute of Agricultural Sciences)
Choi, Hyeon-Yong (Crop Protection Division, National Institute of Agricultural Sciences)
Hong, Su-Bin (Crop Protection Division, National Institute of Agricultural Sciences)
Hur, On-Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences)
Byun, Hee-Seong (Crop Protection Division, National Institute of Agricultural Sciences)
Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Mikyeong (Department of Plant Medicine, Chungbuk National University)
Publication Information
Korean Journal of Environmental Biology / v.39, no.3, 2021 , pp. 319-328 More about this Journal
Abstract
Tomato spotted wilt virus (TSWV) is one of the most destructive viruses worldwide, which causes severe damage to economically important crops, such as pepper and tomato. In this study, we examined the molecular and biological characterization of a TSWV isolate (SW-TO2) infecting tomato and compared it to the recently reported isolates from boxthorn, butterbur, and angelica plants. The phylogenetic analysis based on the complete genome sequences confirmed that SW-TO2 was clustered with those of isolates from boxthorn and pepper in Korea with the maximum nucleotide identities ranging from 98% to 99%. We developed the bioassay method for screening TSWV resistance and tested some commercial pepper and tomato cultivars for resistance evaluation of four isolates of TSWV. TSWV resistance was evaluated as TSWV resistance when all the following three conditions were satisfied: first, when symptoms of necrotic spots or no symptoms were present in the inoculated leaves; second, when there were no symptoms in the upper leaves; and third, when the upper leaves were negative as a result of RT-PCR diagnosis.
Keywords
Tomato spotted wilt virus; bioassay; genome sequence; resistance;
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