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http://dx.doi.org/10.5423/RPD.2017.23.4.383

First Report of Impatiens necrotic spot virus in Hoya carnosa in Korea  

Kim, Mikyeong (Crop Protection Division, National Institute of Agricultural Sciences)
Kang, Hyo-Jung (Chungcheongbuk-do Agricultural Research and Extension Services)
Kwak, Hae-Ryun (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Jeong-Eun (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Jaedeok (Crop Protection Division, National Institute of Agricultural Sciences)
Seo, Jang-Kyun (Graduate School of International Agricultural Technology, Seoul National University)
Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences)
Publication Information
Research in Plant Disease / v.23, no.4, 2017 , pp. 383-387 More about this Journal
Abstract
In 2016, less than 30% of virus-like symptoms such as chlorosis, necrosis and ringspots were observed in Hoya carnosa from commercial greenhouse in Eumseong, Korea. A total of 6 samples from Hoya carnosa were collected both symptomatic and asymptomatic plants and tested for virus infection by RT-PCR of 3 viruses known to infect Hoya spp. including Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Tomato chlorotic spot virus (TCSV). Three symptomatic samples were positives for INSV. Also, it was not the virus detected in three asymptomatic samples. To further confirm the presence of INSV, complete nucleocapsid (N) gene of the virus were amplified and sequenced from two samples. BLAST analysis of the consensus sequence showed that two isolates (INSV-Hy1 and -Hy2) shared nucleotide sequence identities of 99% with each other and 97-99% with other INSV isolates available in the GenbBank. Phylogenetic analysis showed that these isolates closely related to the INSV isolates from ornamental from China. This is the first report of INSV on Hoya carnosa from Korea.
Keywords
Hoya carnosa; Impatiens necrotic spot virus; Orthotospovirus;
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