Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Symptom and Resistance of Cultivated and Wild Capsicum Accessions to Tomato Spotted Wilt Virus

토마토반점위조바이러스에 대한 재배 및 야생형 고추 수집종의 병징과 저항성 조사

  • Han, Jung-Heon (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University) ;
  • Lee, Won-Phil (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University) ;
  • Lee, Jun-Dae (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University) ;
  • Kim, Mi-Kyung (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Choi, Hong-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Yoon, Jae-Bok (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University)
  • 한정헌 ((주)고추와 육종 기업부설연구소) ;
  • 이원필 ((주)고추와 육종 기업부설연구소) ;
  • 이준대 ((주)고추와 육종 기업부설연구소) ;
  • 김미경 (국립농업과학원) ;
  • 최홍수 (국립농업과학원) ;
  • 윤재복 ((주)고추와 육종 기업부설연구소)
  • Received : 2010.11.22
  • Accepted : 2011.03.07
  • Published : 2011.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

One hundred Capsicum accessions were screened for symptomatic response and resistance to Tomato spotted wilt virus-pb1 (TSWV-pb1). Symptom and its severity rating were checked by visual observation at 9, 12, 14, and 45 days after inoculation, respectively. Enzyme-linked immune-sorbent assay was performed all tested individuals on non-inoculated upper leaves after the third rating to indentify viral infection. Leaf curling was predominant in almost susceptible individuals of each accession. Stem necrosis was most frequent in wild species while yellowing in commercial hybrids and Korean land race cultivars. Ring spot, a typical symptom of TSWV, was rarely detected in some of a few accessions. Different levels of resistance to TSWV-pb1 were observed among the tested accessions. High level of resistance was detected in 4 commercial cultivars of Kpc-35, -36, -57, and -62, and 8 wild species of PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, and PI201238 through seedling test by mechanical inoculation.

토마토반점위조바이러스(Tomato spotted wilt virus, TSWV)에 대한 고추 유전자원 및 시판품종 등 100점의 병징 유형과 저항성 반응을 조사하였다. 병징 유형과 TSWV 감염률은 접종 후 9, 12, 14, 45일에 각각 육안으로 조사하였으며, 접종 14일에는 효소면역항체법을 이용하여 실험에 사용한 모든 개체의 비 접종 상엽에서 바이러스 감염 유무를 검정하였다. 감염된 대다수의 개체에서 잎 말림 증상이 가장 빈번하게 관찰되었으며, 국내 상용 $F_1$ 품종과 재래종고추에서는 황화 증상이, 야생형 수집종에서 줄기괴사 증상이 특이적으로 관찰되었다. TSWV의 전형적인 병징으로 알려진 원형반점은 조사한 일부 계통에서 관찰되었고, 계통 내 개체간 바이러스 병징 발현 정도는 상이하였다. 상용 F1 4품종(Kpc-35, -36, -57, -62)과 야생형 고추 8계통(PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, PI201238)은 유묘기에 기계적 접종했을 때 TSWV에 대해 고도의 저항성을 보였다.

Keywords

References

  1. 조점덕, 김정수, 김진영, 김재현, 이신호, 최국선, 김현란, 정봉남. 2005. 채소류의 토마토 반점 위조 바이러스 발생과 병징(I). 식물병연구 11: 213-216. https://doi.org/10.5423/RPD.2005.11.2.213
  2. 조점덕, 김정수, 이신호, 최국선, 정봉남. 2007. 우리나라 고추 바이러스 종류, 병징 및 발생 형태. 식물병연구 13: 75-81. https://doi.org/10.5423/RPD.2007.13.2.075
  3. 최홍수, 이수헌, 김미경, 곽해련, 김정수, 조점덕, 최국선. 2010.2009년 우리나라주요 작물 바이러스병 발생 상황. 식물병연구 16: 1-9. https://doi.org/10.5423/RPD.2010.16.1.001
  4. Boiteux, L. S. 1995. Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense. Theor. Appl. Genet. 90: 146-149.
  5. Boiteux, L. S. and Nagata, T. 1992. Susceptibility of Capsicum chinense PI 159236 to tomato spotted wilt virus isolates in Brazil. Plant Dis. 77: 210.
  6. Boitex, L. S. and de Avila, A. C. 1994. Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense ‘PI 159236’. Euphytica 75: 139-142. https://doi.org/10.1007/BF00024541
  7. Cebolla-Cornejo, J., Soler, S., Gomar B., Soria, M. D. and Nuez, F. 2003. Screening Capsicum germplasm for resistance to tomato spotted wilt virus (TSWV). Ann. Appl. Biol. 143: 143-152. https://doi.org/10.1111/j.1744-7348.2003.tb00280.x
  8. Cho, J.-D., Kim, J.-S., Kim, J.-Y., Choi, G.-S. and Chung, B.-N. 2009. Biological characteristics and nucleotide relationships in Korean Tomato spotted wilt virus isolates. Plant Pathol. J. 25: 26-37. https://doi.org/10.5423/PPJ.2009.25.1.026
  9. Hobbs, H. A., Black, L. L., Johnson, R. R. and Valverde, R. A. 1994. Differences in reactions among tomato spotted wilt virus isolates to three resistant Capsicum chinense lines. Plant Dis. 78: 1220.
  10. Kim, J. H., Choi, G.-S., Kim, J.-S. and Choi, J.-K. 2004. Characterization of Tomato spotted wilt virus from Paprika in Korea. Plant Pathol. J. 20: 297-301. https://doi.org/10.5423/PPJ.2004.20.4.297
  11. Kim, J.-Y., Cho, J.-D., Kim, J.-S., Hong, S.-S., Lee, J.-G., Choi, G.-S. and Lim, J.-W. 2009. Reduction of Tomato spotted wilt virus on Table Tomatoes in Greenhouses by Soil Fumigation Plant Pathol. J. 25: 151-156. https://doi.org/10.5423/PPJ.2009.25.2.151
  12. Margaria, P., Ciuffo, M. and Turina, M. 2004. Resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant pepper cultivars in Almeria, Spain. Plant Pathol. 53: 795. https://doi.org/10.1111/j.1365-3059.2004.01082.x
  13. Moury, B., Selassie-Gebre, K., Marchoux, G., Daubeze, A. M.and Palloix, A. 1998. High temperature effects on hypersensitive resistance to tomato spotted wilt tospovirus (TSWV) in pepper (Capsicum chinense Jacq.). Eur. J. Plant Pathol. 104: 489-498. https://doi.org/10.1023/A:1008618022144
  14. Roggero, P., Masenga, V. and Tavella, L. 2002. Field isolates of Tomato spotted wilt virus overcoming resistance in pepper and their spread to other hosts in Italy. Plant Dis. 86: 950-954. https://doi.org/10.1094/PDIS.2002.86.9.950
  15. Sharman, M. and Persley, D. M. 2006. Field isolates of Tomato spotted wilt virus overcoming resistance in capsicum in Australia. Australasian Plant Pathology 35: 123-128. https://doi.org/10.1071/AP06014
  16. Whitefield, A. E., Ullman, D. E. and German, T. L. 2005. Tospovirus-Thrips interactions. Annu. Rev. Phytopathol. 43: 1-31. https://doi.org/10.1146/annurev.phyto.43.040204.140209

Cited by

  1. Screening of Tomato Spotted Wilt Virus Resistance in Tomato Accessions vol.30, pp.2, 2012, https://doi.org/10.7235/hort.2012.11126