식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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토마토반점위조바이러스에 대한 재배 및 야생형 고추 수집종의 병징과 저항성 조사

Symptom and Resistance of Cultivated and Wild Capsicum Accessions to Tomato Spotted Wilt Virus

  • 한정헌 ((주)고추와 육종 기업부설연구소) ;
  • 이원필 ((주)고추와 육종 기업부설연구소) ;
  • 이준대 ((주)고추와 육종 기업부설연구소) ;
  • 김미경 (국립농업과학원) ;
  • 최홍수 (국립농업과학원) ;
  • 윤재복 ((주)고추와 육종 기업부설연구소)
  • Han, Jung-Heon (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University) ;
  • Lee, Won-Phil (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University) ;
  • Lee, Jun-Dae (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University) ;
  • Kim, Mi-Kyung (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Choi, Hong-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Yoon, Jae-Bok (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, CALS, Seoul National University)
  • 투고 : 2010.11.22
  • 심사 : 2011.03.07
  • 발행 : 2011.04.30
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초록

토마토반점위조바이러스(Tomato spotted wilt virus, TSWV)에 대한 고추 유전자원 및 시판품종 등 100점의 병징 유형과 저항성 반응을 조사하였다. 병징 유형과 TSWV 감염률은 접종 후 9, 12, 14, 45일에 각각 육안으로 조사하였으며, 접종 14일에는 효소면역항체법을 이용하여 실험에 사용한 모든 개체의 비 접종 상엽에서 바이러스 감염 유무를 검정하였다. 감염된 대다수의 개체에서 잎 말림 증상이 가장 빈번하게 관찰되었으며, 국내 상용 $F_1$ 품종과 재래종고추에서는 황화 증상이, 야생형 수집종에서 줄기괴사 증상이 특이적으로 관찰되었다. TSWV의 전형적인 병징으로 알려진 원형반점은 조사한 일부 계통에서 관찰되었고, 계통 내 개체간 바이러스 병징 발현 정도는 상이하였다. 상용 F1 4품종(Kpc-35, -36, -57, -62)과 야생형 고추 8계통(PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, PI201238)은 유묘기에 기계적 접종했을 때 TSWV에 대해 고도의 저항성을 보였다.

One hundred Capsicum accessions were screened for symptomatic response and resistance to Tomato spotted wilt virus-pb1 (TSWV-pb1). Symptom and its severity rating were checked by visual observation at 9, 12, 14, and 45 days after inoculation, respectively. Enzyme-linked immune-sorbent assay was performed all tested individuals on non-inoculated upper leaves after the third rating to indentify viral infection. Leaf curling was predominant in almost susceptible individuals of each accession. Stem necrosis was most frequent in wild species while yellowing in commercial hybrids and Korean land race cultivars. Ring spot, a typical symptom of TSWV, was rarely detected in some of a few accessions. Different levels of resistance to TSWV-pb1 were observed among the tested accessions. High level of resistance was detected in 4 commercial cultivars of Kpc-35, -36, -57, and -62, and 8 wild species of PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, and PI201238 through seedling test by mechanical inoculation.

키워드

참고문헌

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피인용 문헌

  1. Screening of Tomato Spotted Wilt Virus Resistance in Tomato Accessions vol.30, pp.2, 2012, https://doi.org/10.7235/hort.2012.11126