Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Impatiens necrotic spot virus in Hoya carnosa in Korea

호야에서 발생한 봉선화괴저반점바이러스 국내 첫 보고

  • Kim, Mikyeong (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kang, Hyo-Jung (Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Kwak, Hae-Ryun (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Jeong-Eun (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Jaedeok (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Seo, Jang-Kyun (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences)
  • 김미경 (국립농업과학원 작물보호과) ;
  • 강효중 (충청북도농업기술원) ;
  • 곽해련 (국립농업과학원 작물보호과) ;
  • 김정은 (국립농업과학원 작물보호과) ;
  • 김재덕 (국립농업과학원 작물보호과) ;
  • 서장균 (서울대학교 국제농업기술학과) ;
  • 최홍수 (국립농업과학원 작물보호과)
  • Received : 2017.10.26
  • Accepted : 2017.11.01
  • Published : 2017.12.31
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Abstract

In 2016, less than 30% of virus-like symptoms such as chlorosis, necrosis and ringspots were observed in Hoya carnosa from commercial greenhouse in Eumseong, Korea. A total of 6 samples from Hoya carnosa were collected both symptomatic and asymptomatic plants and tested for virus infection by RT-PCR of 3 viruses known to infect Hoya spp. including Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Tomato chlorotic spot virus (TCSV). Three symptomatic samples were positives for INSV. Also, it was not the virus detected in three asymptomatic samples. To further confirm the presence of INSV, complete nucleocapsid (N) gene of the virus were amplified and sequenced from two samples. BLAST analysis of the consensus sequence showed that two isolates (INSV-Hy1 and -Hy2) shared nucleotide sequence identities of 99% with each other and 97-99% with other INSV isolates available in the GenbBank. Phylogenetic analysis showed that these isolates closely related to the INSV isolates from ornamental from China. This is the first report of INSV on Hoya carnosa from Korea.

2016년 충북 음성의 호야 농가에서 30% 미만으로 퇴록, 괴사 및 원형반점 등 바이러스 같은 증상이 관찰되었다. 바이러스 의심 증상 및 건전주로 보이는 호야 6 시료를 채집하였고, 이들 시료에 대한 바이러스 감염 여부를 확인하기 위하여 호야 속 식물의 감염이 확인된 토마토반점위조바이러스(TSWV), 봉선화괴저반점바이러스(INSV) 및 토마토퇴록반점바이러스(TCSV) 3종 특이 진단 프라이머를 이용하여 RT-PCR 수행하였다. 그 결과 원형, 괴사 반점 등 증상을 보인 호야 3 시료에서 봉선화괴저반점바이러스(INSV) 양성반응을 보였고, 증상이 보이지 않는 호야 3 시료에서 음성반응을 보였다. 호야 2 시료에서 대하여 추가적으로 봉선화괴저반점바이러스(INSV) 감염을 확인하기 위하여 전체 핵단백질(N) 유전자 염기서열을 증폭하였고, 염기서열을 얻었다. 호야의 봉선화괴저반점바이러스(INSV) Hy-1과 Hy-2에서 얻어진 공통염기서열의 BLAST 분석 결과 두 분리주 간은 99% 상동성을 보였고, Genbank에 등록된 봉선화괴저반점바이러스(INSV) 분리주들과는 97-99% 염기서열 상동성을 보였다. 계통학적 유연관계분석 결과 호야 분리주는 중국의 화훼류 분리주들과 밀접한 유연관계를 가지는 것을 확인하였다. 본 보고는 국내에서 처음으로 호야에서의 봉선화괴저반점바이러스(INSV) 보고이다.

Keywords

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