• Journal of Animal Science and Technology
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• v.53 no.2
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• pp.177-182
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• 2011

The objective of this study was to examine the effect of HACCP implementation on the medicine use, antibiotic utilization in each feeding stage and productivity of large scale swine farms (over 5,000 pigs) in Korea. Data were collected from ten swine farms before and after implementation of a HACCP system. Total number of piglets, number of initial weaning piglets, number of weaning piglets, survival rate of piglets, date of weaning piglets, number of piglets per sow per year and price for medicine used per month on HACCP implemented swine farms had a tendency to increase without any significant difference. However, parturition rate, market pig per sow per year, number of antibiotic used and farm number of feeds containing antibiotics at fattening stage added at the swine farm were significantly increased after HACCP implementation (p<0.05). Due to increase in the feed cost recently, production cost for shipment in HACCP system implemented swine farms had increased in this study. In conclusion, our results indicated that the implementation of HACCP system might have many effects including reduction of medical expenses and improvement of productivity of the pig farms.

• 동물약계
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• no.94
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• pp.3-5
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• 2004

[ $\cdot$ ]니트로푸란제 동물용의약품 자율 수거 $\cdot$가축전염병 발생 신고자 신원 비공개 조치 $\cdot$축산용항생제 관리시스템 관련 협의회 참석 $\cdot$동물약사감시 행정처분 결과 홍보 $\cdot$제2차 이사회 개최 $\cdot$소독약품 정비관련 소위원회 회의 참석 $\cdot$동물약품 GMP실무반 교육 실시 $\cdot$양계용 동물약품 북한 지원 $\cdot$동물용 마취제 판매관리 강화 협조 요청 $\cdot$방역용 소독약품 시장거래가격 조사 $\cdot$동물약품 마케팅반 교육 실시 $\cdot$제3차 이사회 개최 $\cdot$동물약품제조용 양허관세적용 유당 증량 배정 $\cdot$수출 전략 협의회 개최 $\cdot$아시아$\cdot$태평양 수의사총회 개최 안내 $\cdot$조합 2004년도 상반기 알찬거래선 선정

• 동물약계
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• no.97
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• pp.3-6
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• 2004

[ $\cdot$ ]동물약사감시 행정처분 결과 홍보 $\cdot$수입백신 특별소위원회 구성 $\cdot$축산용 항생제 관리시스템 구축 협의회 참석 $\cdot$동물약품업계 발전을 위한 정책토론회 참석 $\cdot$임시 이사회 개최 $\cdot$중국산 동물약품 GMP 관련 수입시 유의사항 $\cdot$가축전염병 예방약류 사양서 제출 $\cdot$3$\cdot$4/4분기 가축전염병 중앙예찰협의회 참석 $\cdot$부작용 수입 동물약품 회수 및 잠정 수입중단 $\cdot$현장문제 해결을 위한 연구과제 제안 $\cdot$검역원, 잔류물질 관리 국제 심포지엄 개최 $\cdot$대체의학 특별소위원회 개최 $\cdot$동물약품 사용 절감대책 추진회의 참석 $\cdot$동물약품제조용 양허관세적용 유당 변경 배정 $\cdot$산업자원부 해외전시회 단체참가 지원 신청 $\cdot$IFAH, 사료첨가제 감축 관련 의견 제시 $\cdot$제5회 KAHPA컵 골프대회 개최 $\cdot$조합 2004년도 하반기 알선품목 선정 $\cdot$2004 국제 애완동물 및 용품 전시회 개최

• Korean Journal of Environmental Agriculture
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• v.30 no.1
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• pp.68-75
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• 2011

BACKGROUND: To assess and prevent the environmental impacts and risks by veterinary pharmaceuticals, Guidance on Estimating Soil Persistence and Degradation Kinetics from Environmental Fate Studies on Veterinary Pharmaceuticals for Environmental Risk Assessment was proposed. METHODS AND RESULTS: Proposed guidance was coined by VICH, EU guideline, OECD guideline and soil dissipation studies for the purpose of international harmonizing. Guidance was also modified from pesticide soil persistence testing guidelines of US, EU, and Korea, with practical approaches adopting in-use test guideline for Korea. CONCLUSION(S): Proposed guidance are consisted of three parts; Laboratory Soil Experiment, Field Soil Dissipation Study, and Estimation of $DT_{50}/DT_{90}$. Proposed guidance is to be available for the requirement for registration of veterinary pharmaceuticals with fit for purpose in Korea.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.331-337
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• 2005

한우와 홀스타인의 분변으로부터 MRS배지와 LAPT배지를 이용하여 무작위 선발법으로 54균주의 유산균을 1차로 분리 하였다. 1차로 분리된 54균주에 대해 내담즙성이 우수한 10균주를 분리 한 다음 내산성을 조사한 결과 인공위액 pH2.5에서 LS1, LS15 및 LL6 균주가 각각 66.5%, 82.6% 및 80.7%의 생존율을 나타내었다. Sal. typhimurium, Sta. aureus 및 Cl. perfringens 의 병원균에 대해 가장 큰 항균력을 보인 균주는 LL6와 LL7이었다. API CHL kit로 동정한 결과 LS1, LS2 및 LM1 균주는 모두 L. fermentum, LL6와 LL7 균주L. acidophilus, LS3 균주는 L. plantarum으로 각각동정 되고, 나머지 4균주는 Lactobacillus sp. 로 동정되어 분리된 10균주 모두 안전성 있는 유산 간균임을 확인하였다. 10종류의 항생제에 대한 내성을 조사한 결과 ampicillin, amoxicillin and erythromycin 에 대해서는 억제되었으나 colistin과 ciprofloxacin에 대해 모두 내성을 나타내었다. LB1, LL6 및 LL7 균주는 gentamicin과 neomycin에 대해 내성을 보여 주었다. 분리 동정된 균주 중에 내산성, 내담즙성 및 병원성균에 대한 항균력이 우수한 것으로 probiotic 유산균으로 사용가능성이 높은 것은 LL6인 L. acidophilus 로 나타났다.

• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.207-216
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• 2009

This experiment was conducted to investigate the effects of dietary supplementation of organic acid mixture on growth performance, cecal microflora, blood characteristics and immune response in broiler chicks and to prove the possibility of organic acid mixture as an alternative to antibiotics growth promotor. A total of four hundred eighty, 1-day-old male broiler chicks (Ross$\times$Ross 308) were randomly divided into 4 groups with 4 replicates of 30 birds each. The treatments were NC (free antibiotics), PC (basal diet with virginiamycin 10 ppm and salinomycin 60 ppm), 0.3% organic acid, and 0.5% organic acid. The final body weight and body weight gain were significantly higher in organic acid 0.5% than NC (P<0.05). The feed conversion ratio in all treated groups were significantly improved as compared to that of NC (P<0.05). The carcass rate and relative organs weight were not significantly difference among the groups. The relative weight and length of small intestine in PC were significantly decreased than the other groups. The numbers of cecal coliform bacteria and Salmonella in all treated groups were significantly lower than NC (P<0.05). The number of cecal lactic acid bacteria was not different among the groups. No significant differences among the groups were observed in the contents of total cholesterol, triglyceride, blood urea nitrogen (BUN), albumin, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in blood serum. The contents of total protein and globulin in blood serum of PC and organic acid treated groups were significantly increased as compared to those of NC (P<0.05). Therefore, albumin:globulin ratio of PC and organic acid treated groups was significantly lower than NC (P<0.05). The total white blood cell (WBC), heterophil, lymphocyte, and stress indicator (heterophil:lymphocyte ratio) were not significantly different among the groups. No significant difference was observed on the expression rate of splenic cytokines mRNA in organic acid treated groups compared to the control. Consequently, supplemental organic acid mixture improved the growth performance, and influenced positive effects on the intestinal microflora by inhibiting the growth of harmful bacteria without any adverse effects on relative weights of organs and blood biochemical parameters in broiler chicks.

• Journal of Animal Science and Technology
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• v.50 no.4
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• pp.509-518
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• 2008

This experiment was conducted to investigate enzyme activity, antimicrobial activity, and antibiotics susceptibility of Lactobacilli strain(Lactobacillus reuteri BLA5, Lactobacillus crispatus BLA7, Lactobacillus reuteri BLA9, Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9, Lactobacillus vaginalis LLA11) isolated from chicken ceca and were selected by organic acid synthesis, acid tolerance, bile salt tolerance. The enzymes activities were different among strains of Lactobacilli. The amylase activity and lipase activity of Lactobacillus were high but cellulase activity and protease activity of that were low. Lactobacillus culture showed high antimicrobial activity against E. coli but low antimicrobial activity against Salmonella. The inhibitory factor of Lactobacilli isolated from chickens’ cecum on E. coli was low pH by organic acid. All of Lactobacillus isolated from chicken’s cecum were susceptible to ampicillin and amoxicillin but weren’t susceptible at the optimum level of feed additive antibiotics(virginiamycin and salinomycin).

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.226-226
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• 2021

Tetractycline은 sulfonamides, penicilines 등과 함께 축산계에서 널리 사용되는 대표적인 항생물질의 한 종류이다. 2011년 사료 내 항생제 투여를 금지한 이후 자가치료 및 예방용으로 사용되고 있으며, 가축의 체내로 투여되는 tetracycline는 주로 분뇨에 포함되어 배출되는데, 강우 등 물 순환에 따라 지표수 및 지하수로 이동하여 미생물에 독성을 일으키거나 내성균이 발생하기도 한다. Tetracycline 등의 항생물질 처리 방식으로 흡착 등 다양한 공정이 제시되고 있다. 본 연구에서는 산화제 중 하나인 ferrate(VI)를 이용하여 tetracycline 분해실험을 수행하였다. ferrate(VI)는 염소산화물 및 H₂O₂에 비해 비교적 강한 산화력을 가지며, 처리 후 발생되는 철염(Fe³⁺)은 독성이 없다는 장점이 있다. Ferrate(VI)는 병원균 제거 등에 효과적인 것으로 알려져 있으며, 다양한 난분해성 물질과 항생물질을 성공적으로 분해하여 그 효과를 입증한 바 있다. 본 연구에서는 자체적으로 제조한 potassium ferrate(VI)를 이용하여 다양한 수중 환경에서 tetracycline를 분해하고, 분해특성 및 중간생성물 연구를 수행하였다. Ferrate(VI)는 염기성 환경에서 tetracycline 분해효율이 가장 높은 것으로 나타났으며, 이는 pH에 따른 tetracycline과 ferrate(VI)의 이온화가 가장 큰 원인인 것으로 판단된다. 특히 ferrate(VI)는 pH가 낮을수록 쉽게 환원되는 특징이 있으며, 염기성으로 갈수록 안정화하여 오래 잔류하므로 이러한 결과가 나타난 것으로 판단된다. 중간생성물 조사 결과, ferrate(VI)와 tetracycline 사이의 분해 메커니즘은 주로 OH 라디칼로 인한 것이 대부분이며, hydroxylation과 amino group에서의 demethylation의 형태로 발생하였다. 이후 추가적인 반응으로 benzene ring이 깨지면서 결과적으로 CO₂ 및 H₂O 등으로 무기물화 되는 것으로 판단된다.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.307-314
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• 2006

To search direct fed microbials, we isolated a Candida sp. from kefir grain. The isolated Candida sp. strain showed 99.8% of identity to the species of Candida kefyr by API 20C kit. Enzyme activity of Candida kefyr was higher in amylase (0.33±1.12μmol/min/mg) than that in phytase (0.052±0.98μmol/ min/mg) cellulase(0.051±μmol/min/mg) and xylanase (0.011±0.98mol/min/mg). The maximum numbers of Candida kefyr in growth curve were reached at 30 h fermentation. Candida kefyr showed high resistances to acidic environment, which was not perfectly extincted even at pH 2.0. And it showed high tolerance to bile salt which had almost 97.2% of survival in the presence of 1.0% bile salt.Especially, Candida kefyr showed high heat stability which remained 10% of initial microorganisms at 60℃. Candida kefyr was not generally inhibited by most of 11 antibiotic agent which contained tetracycline groups. These results suggest that the isolated Candida kefyr has a useful properties as probiotics.

• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.308-314
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• 2010

This study was conducted to investigate the characteristics and concentrations of ammonia produced from the livestock swine nightsoil treated with or without the livestock - environment improving agents. Odor generating device made of acryl was made by volume of 96 L to sample the ammonia odor. When swine night soil was placed in the device, concentration of ammonia averaged out at about 23.4 ppmv and ranged from 16 ppmv to 40 ppmv. Removal efficiencies of them showed 50% to 90% as compared to initial level before spraying, when the spray type agents were used immediately after they purchased. The persistence of the efficiency was retained for first two days. Among the agents, the natural deodorant showed the best efficiency of 87 to 99%. To evaluate the effects of 5 kinds of dietary probiotic powders, the experiments were conducted and based dietary treatments without antibiotics on growing piglets. In experiments, 60 piglets ($6.3{\pm}0.2\;kg$) were subjected to a 35-day feeding trial in which the effects of the dietary probiotic powder on the ammonia emission were compared. The ammonia gas emission was measured for every week. Ammonia emission from the swine nightsoil obtained from piglets supplemented with the probiotics power was lower than that of the nightsoil obtained from pigs in the control treatment (without probiotics). In ammonia removal efficiencies of the experimental groups, some products showed from 71% to 99% removal efficiencies throughout the entire period as compared to the control group. On the other hand, initial reduction of ammonia in some product was effective temporarily. After then, it did not show any reduction efficiency of ammonia.