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http://dx.doi.org/10.5187/JAST.2008.50.4.509

Characterization of Lactobacilli Isolated from Chicken Ceca as Probiotics  

Kim, Sang-Ho (National Institute of Animal Science, R.D.A)
Kim, Dong-Wook (National Institute of Animal Science, R.D.A)
Park, Su-Young (Nonghyup Feed Inc.)
Kim, Ji-Hyuk (National Institute of Animal Science, R.D.A)
Kang, Geun-Ho (National Institute of Animal Science, R.D.A)
Kang, Hwan-Ku (National Institute of Animal Science, R.D.A)
Yu, Dong-Jo (National Institute of Animal Science, R.D.A)
Na, Jae-Cheon (National Institute of Animal Science, R.D.A)
Lee, Sang-Jin (National Institute of Animal Science, R.D.A)
Publication Information
Journal of Animal Science and Technology / v.50, no.4, 2008 , pp. 509-518 More about this Journal
Abstract
This experiment was conducted to investigate enzyme activity, antimicrobial activity, and antibiotics susceptibility of Lactobacilli strain(Lactobacillus reuteri BLA5, Lactobacillus crispatus BLA7, Lactobacillus reuteri BLA9, Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9, Lactobacillus vaginalis LLA11) isolated from chicken ceca and were selected by organic acid synthesis, acid tolerance, bile salt tolerance. The enzymes activities were different among strains of Lactobacilli. The amylase activity and lipase activity of Lactobacillus were high but cellulase activity and protease activity of that were low. Lactobacillus culture showed high antimicrobial activity against E. coli but low antimicrobial activity against Salmonella. The inhibitory factor of Lactobacilli isolated from chickens’ cecum on E. coli was low pH by organic acid. All of Lactobacillus isolated from chicken’s cecum were susceptible to ampicillin and amoxicillin but weren’t susceptible at the optimum level of feed additive antibiotics(virginiamycin and salinomycin).
Keywords
Lactobacillus; Enzyme activity; Antimicrobial activity; Antibiotics susceptibility
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1 Fuller, R. 1975. Nature of the determinant responsible for the adhesion of lactobacilli to chicken crop epithelial cells. Fournal of General Microbiology. 87:245-250   DOI   ScienceOn
2 Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378   DOI
3 Bauer, A. W., Kirby, W. M. J. C., Sherris, J. C. and Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45:493-496   DOI
4 Bongaerts, G., Severijne, R. and Timmerman, H. 2005. Effect of antibiotics, prebiotics and Probiotics in treatment for hepatic encephalopathy. Med. Hypotheses. 64:64-68   DOI   ScienceOn
5 Dunham, H. J., William, C., Edens, F. W., Casas, I. A. and Dobrogosz, W. J. 1993. Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys. Poult. Sci. 72(Suppl 1):103(abstract)
6 Afrin, R., Haruyama, T., Yanagida, Y., Kobatake, E. and Aizawa, M. 2000. Catalytic activity of Teflon particle-immobilized protease in aqueous solution. J. Molecular Catalysis 9:259-267   DOI   ScienceOn
7 Ransac, S., Blaauw, M., Lesuisse, E., Schanck, K., Colson, C. and Dijkstra, B. W. 1994. Crystalliza subtilis. J. Molecular Biology. 238:857-859   DOI   ScienceOn
8 Rubio, L. A., Brenes, A., Setien tion and Preliminary X-ray Analysis of a Lipase from Bacillus, I., de la Asuncion, G., Duran, N. and Cutuli, M. T. 1998. Lactobacilli counts in crop ileum and caecum of growing broiler chickens fed on practical diets containing whole or dehulled sweet lupin Lupinus angustifolius seed meal. Br. Poult. Sci. 39:354-359   DOI   ScienceOn
9 Spencer, R. J. and Chesson, A. 1994. The effect of Lactobacillus spp. on the attachment of enterotoxigenic Escherichia coli to isolated porcine enterocytes. Fournal of Applied Bacteriology. 77:215-220   DOI
10 Tagg, J. R., Dajani, A. S. and Wannamaker, L. W. 1976. Bacteriocins of Gram-positive bacteria. Bacteriological Reviews 40:722-756
11 Miller, G. L. 1959. Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-428   DOI
12 Hernandez, F., Madrid, J., Garcia, V., Orengo, J. and Megias. M. D. 2004. Influence of two plant extracts on broilers performance, digestibility, and organ size. Poult. Sci. 83:169-174   DOI
13 Jennson, B. 1993. The possibility of manipulation of microbial activity in the digestive tract of monogastric animals. Proceedings of the 44th annual meeting for animal production. p 313
14 Klaenhammer, T. R. 1988. Bacteriocins of lactic acid bacteria. Biochimie 70:337-349   DOI   ScienceOn
15 Fuller, R. 1973. Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl. J. Appl. Bacteriol. 36:131-139   DOI
16 Gibson, G. R. and Fuller, R. 2000. Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. J. Nutr. 130:391-395
17 Gilliland, S. E. 1979. Beneficial interrelationships between certain micro-organisms and humans: Candidate micro-organisms for use as dietary adjuncts. J. Food Protect. 42:164   DOI
18 Watkins, B. A., Miller, B. F. and Neil, D. H. 1982. In vivo effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks. Poult. Sci. 61:1298-1308   DOI
19 Watkins, B. A. and Kratzer, F. H. 1983. Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks. Poult. Sci. 62:2088-2094   DOI   ScienceOn
20 White, F., Wenham, G., Sharman, G. A., Jones, A. S., Rattray, E. A. and McDonald, I. 1969. Stomach function in relation to a scour syndrome in the piglet. Br. J. Nutr. 23:847-858   DOI   ScienceOn
21 Barrow, P. A., Brooker, B. E., Fuller, R. and Newport, M. J. 1980. The attachment of bacteria to the epithelium of the pig and its importance in the microecology of the intestine. J. Appl. Bact. 48:147   DOI
22 Jin, L. Z., Ho, Y. W., Abdullah, N., Ali, M. A. and Jalaudin, S. 1996. Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken. Letters in Applied Microbiology. 23:67-71   DOI   ScienceOn
23 Mohan, B., Kadirvel, R., Natarajan, A. and Bhaskaran, M. 1996. Effect of probiotic supplementation on growth nitrogen utilization and serum cholesterol in broilers. Br. Poult. Sci. 37:395-401   DOI   ScienceOn
24 Nurmi, E. and Rantala, M. 1973. New aspects of Salmonella infection in broiler production. Nature. 241:210-211   DOI   ScienceOn
25 Morishita, Y., Fuller, R. and Coates, M. E. 1982. Influence of dietary lactose on the gut flora of chicks. Br. Poult. Scie. 23:349-359   DOI   ScienceOn