• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.2 s.57
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• pp.117-121
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• 2006

Although many studies have been performed to elucidate the molecular consequence of factors that regulate skin aging, little is known about the effect of green tea catechins except EGCG. The matrix metalloproteinase (MMP), can degrade matrix proteins and results in a collagen deficiency in photodamaged skin, are known to play an important role in photoaging. This study, investigated the effects of green tea catechins on the UVA-induced MMP-1 expression, activity of MMP-2 and synthesis of type I procollagen in human dermal fibroblasts. We examined eight catechins that naturally exist in green tea leaves and compared their efficacies among them. Most of catechins inhibited the expression of MMP-1 in dose dependent manner, and the levels were reduced, especially, 57.4 and 68.2% by treatment with $1{\mu}M$ of epigallocatechin-3-gallate (EGCG) and gallocatechin-3-gallate (GCG), respectively. Also, catechins significantly suppressed the activities of MMP-2. Catechins also induced the expression of type I procollagen, however, they acted only at the concentration below $1{\mu}M$ interestingly. Furthermore, when EGCG:GCG:ECG had the ratio of 0.5:1.5:.1.3, they presented the most effective on procollagen synthesis. Therefore, we concluded that catechins significantly inhibited MMPs and induced collagen synthesis. Taken together, all these results suggested that green tea catechins might be good natural materials act as an anti-photoaging and a skin-aging improving agent.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.3
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• pp.297-305
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• 2014

Red ginseng (RG) contains specific ginsenosides (Rg2, Rg3) which show various pharmacological effects and absorption rate in the body better than panax ginseng. Therefore many people have been used it for health for a long time. Furthermore, many researchers have been studying its biological activities for a long times because fermentation generates lots of beneficial small molecules good for health. In this study, we fermented red ginseng with mycelium of Leatiporus sulphures var. miniatus for 7 days. As a result, we found that three ginsenosides Rg1, Re and Rb2 were decreased from 0.24, 0.25, 0.16 mg/g to 0.12, 0.1, 0.03 mg/g respectively HPLC analysis. In addition, we studied biological activities of fermented red ginseng (FRG) about skin ageing such as anti-inflammation, cell migration, anti-oxidation, collagen type 1 synthesis, and MMP-1 inhibition activities. As a result, FRG were shown higher anti-inflammatory and cell migration promoting activities than RG. FRG inhibited production of nitric oxide (NO) and mRNA expression of inducible nitric oxide synthase (iNOS) and decreased interleukin (IL)-6 induced by LPS stimulation in RAW 264.7 cells. In conclusion, this study suggest that FRG could be a potential source as a new natural anti-inflammatory agent.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.2
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• pp.133-145
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• 2020

This study utilized nano-liposomes for the purpose of stabilizing and increasing the solubility of biotin, a water-soluble active material with low solubility. The particle size, zeta potential, and polydispersity index were confirmed with a nano zetasizer. It was possible to manufacture nano liposomes at 100 to 250 nm of particle size and -80 to -30 mV of zeta potential. Dialysis membrane method (DMM) was used to measure the capsulation efficiency of biotin in biotin nano-liposomes, and results showed that pH increased biotin nano-liposomes had higher capsulation efficiency than normal biotin nano-liposome. Through this experiment, it was confirmed that the pH has a great influence on the stability of biotin nano-liposomes. In vitro franz diffusion cell method was used to measure in vitro skin absorption rate of biotin nano-liposomes. The shape of the formulation and biotin solubility in nano-liposome was observed by cryogenic transmission electron microscopy (cryo-TEM). Through this study, we confirmed that biotin, which is introduced as closely related to hair health, can be incorporated into a nano-liposome drug delivery system, to make biotin nano-liposome with improved solubility and precipitation problems.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.389-392
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• 2004

We screened several materials to stimulate IGF-1 promoter activity using luciferase reporter assay and found that Amomi Semen extract (ASE) among them is the most powerful stimulator We also studied about the anti-wrinkle effect of ethanolic extract of Amoni Semen in vitro and in vivo. Semi-quantitative RT-PCR showed that the extract elevated the presence level of IGF-1 mRNA. And $[^3H]$ proline incorporation and semi-quantitative RT-PCR showed that the extract increased the expression of type-I collagen compared with vehicle in vitro and in vivo, respectively. Significant inhibition of MMP-1 expression was determined by ELISA and Western blot. Finally, topical treatment of the extract on hairless mouse's dorsal skin expanded the volume of collagen and dermal thickness. These results suggest that Amomi Semen may be a good candidate for improving extracellular matrix through the increase of collagen expression and inhibition of MMP-1 expression. Moreover, this study enables us to guess that IGF-1 stimulated by the extract may be involved in the mechanism of anti-wrinkle effect of it.

• Journal of Mushroom
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• v.16 no.4
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• pp.318-323
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• 2018

This study investigated whether Ganoderma lucidum (Y2)-mediated fermentation of Artemisia capillaris extract (ACE) could synergistically enhance its antioxidant, anti-inflammatory, and tyrosinase-inhibiting activities. Both G. lucidum extract and fermented ACE exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, but with poorer efficacy than ACE (even at a low ACE concentration). Viability of RAW264.7 macrophages was significantly reduced in the presence of ACE (150 mg/mL and above). However, this effect was greatly mitigated upon G. lucidum-mediated ACE fermentation. Additionally, relative to the same concentration ($25{\mu}g/mL$) of G. lucidum mycelial extract, ACE exhibited an improved ability to significantly inhibit RAW264.7 macrophage nitric oxide (NO) production. Finally, relative to the same concentration ($200{\mu}g/mL$) of a positive control (arbutin), fermented ACE exhibited an approximately 3.66 times higher capacity for tyrosinase inhibition. These results suggest that G. lucidum-fermented ACE possesses enhanced tyrosinase-inhibiting activity and may be of utility as a skin-lightening agent.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.163-170
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• 2021

This study was conducted to create a technology to remove acne bacteria with human-friendly materials. First, the Cutibacterium acnes (C. acnes) were adsorbed to the mica disc to grow, and then the biofilm was checked through an atomic microscope to see if the biofilm had grown. Based on the topographic image, the shape changed round, the size was 17% longer on average, and the phase value of the resonance frequency separating materials was observed as a single value, the biofilm grown by covering the extracellular polymeric substrate (EPS). As a result of processing 50 mM of amino acids in the matured biofilm, the concentration of C. acnes decreased when valine, serine, arginine and leucine were treated. Scanning with nanoindentation and AFM contact modes confirmed that the hardness of biofilms treated with Valine (Val) increased. This indicates that an AFM tip measured cell which may have more solidity than that of EPS. The experiment of fluorescent tagged to EPS displays an existence of EPS at the condition of 10 mM Val, but an inhibition of growth of EPS at the 50 mM Val. Number of C. acnes was also reduced above 10 mM of Val. Weak adhesion of biofilm generated from an inhibition of EPS formation seems to induce decrease of C. acnes. Accordingly, we elucidated that Val has an efficiency which eliminates C. acnes by approach of an inhibition of EPS.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.1
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• pp.77-85
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• 2022

Retinaldehyde (RA), vitamin A derivative, is an intermediate between retinol and retinoic acid and has an excellent wrinkle improving effect. In this study, Drug-in-cyclodextrin-in-liposome (DCL) was used to enhance the stability and skin penetration of RA. The complex of RA and hydroxypropyl-beta-cyclodextrin (HP-β-CD) was prepared by the freeze-drying method, and the presence or absence of inclusion of retinal was confirmed by UV-Vis spectrometer, FT-IR and SEM images. RA was captured in HP-β-CD about 95.6% on 1 : 15 (w/w). The retinal-HP-β-CD complex was encapsulated in liposomes using a homomixer and microfluidizer, with an average particle size of 215 ± 4.2 nm and a zeta potential of -31.2 ± 0.5 mv. In the evaluation of the degradation stability of RA, degradation rate of RA-HP-β-CD-liposomes in water was 1.8% higher than RA-liposome (5.8%), RA-HP-β-CD complex (9.7%) and RA alone (37.6%). RA cream (0.05% RA) including RA-HP-β-CD-liposomes was prepared for clinical test with wrinkle-improving efficacy and skin dermis denseness evaluated for 2 or 4 weeks. RA cream showed a significant wrinkle improving effect without skin irritation. In conclusion, it was confirmed that the double stabilization technology using the DCL system contribu tes to the effect of improving skin wrinkles by increasing the stabilization of retinal.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.2
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• pp.103-110
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• 2024

Lipid nanoparticles (LNPs) are a stable and an effective system that protects cell-impermeable biologically active compounds such as nucleic acids, proteins, and peptides against degradation caused by subtle environmental changes. This study focuses on developing LNPs encapsulating gallic acid (GA), an antioxidant, to effectively prolong the half-life of tetrahexyldecyl ascorbate (THDC), a oil-soluble vitamin C derivative. These LNPs were synthesized in small, uniform sizes at room temperature and pressure conditions using a microfluidics chip. Compared to liposomes manufactured under high pressure and high temperature conditions through conventional microfluidizers, LNPs manufactured through microfluidics chips had excellent dispersion and temperature stability, and improved skin absorption as well as improved oxidative stability of fat-soluble vitamin C derivatives. Future studies will focus on ex vivo and in vivo evaluations to study skin improvement to further validate these results.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.49-54
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• 2009

Rhapontin is the glycosylated stilbene compound, and comprising major component of rhubarb root extract. Rhapontin has been used as a raw material of skin-whitening cosmetics in Korea. Rhapontigenin, the aglycone of rhapontin, has been suggested to be more active than its glycosylated form. Therefore, the rhubarb root extract was treated with commercial enzyme, Pectinex to remove glycosylated moiety of rhapontin and rhapontigenin was prepared. The resulting material was analysed and identified as rhapontigenin by proton NMR and MALDI-Mass. Rhapontigenin exhibited tyrosinase inhibitory activity with an $IC_{50}$ of $126.72{\mu}g/mL$. The tyrosinase inhibitory activity of rhapontigenin was six times higher than that of rhapontin. In melanin biosynthesis inhibition assay using Streptomyces bikiniensis, rhapontigenin showed wider inhibition zone than that of rhapontin. From these results, we expect that rhapontigenin has stronger skin whitening effect than rhapontin and has advantages in cosmetic industry.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2001.11a
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• pp.14-14
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• 2001

현재 제지용 펄프 및 펼프 원료의 국내 자급상태는 매우 낮은 수준이다. 따라서 원료 가격변동과 환율에 따라 수익성은 물론, 원료 수급에도 큰 영향을 받게 된다. 최근 버 진펄프의 가격상승은 재생펄프 사용량과 고지수입량을 더욱 늘리고 있으며, 이들의 사 용에 따른 품질저하 개선노력도 꾸준히 행해지고 있다. 그러나 원가절감과 품질유지라 는 두 마리 토끼를 한꺼번에 잡는 것은 그리 쉬운 일이 아니며 그 효과 또한 점점 미 미해 지고 있는 실정이다. 외국의 경우 이러한 문제점들을 펄프소재면에서 접근하여 해 결하고자 하는 시도가 있었다. 중국은 벗짚펄프를 제지용으로 적용하여 오랜 시행착오 끝에 성공을 거두고 있으며, 남미에서도 수수 등을 이용한 제지용 펼프 개발에 노력과 투자를 아끼지 않고 있다. 이는 제한된 원료의 사용만으로 직면하고 있는 여러 문제점 들을 해결하기가 힘들다는 언식에서 출발한 것이며, 새로운 원료개발 자체가 그 해결방 안으로서 충분한 가치가 있음을 보여준다고 하겠다. 이와 같은 맥락에 따라 국내에서 적용이 가능한 소재를 조사하였으며 우선적으로 신서란을 선택하게 되었다. 신서란은 뉴질랜드가 원산지로 알려져 있으며 오래전부터 제주도에 자생하고 있는 다년생 초본류이다. 수많은 경질섬유(hard fiber)중에서도 경제적으로 재배되는 유일한 작물로 선박용 로프, 그물, 범포 등의 원료로 사용되었으나 현재는 화학섬유로 대체되 어 그 사용이 전무한 실정이다. 선서란은 고강도와 우수한 유연성, 탄력성 및 양호한 광학적 성질을 가지고 있으며 무엇보다 척박한 땅에서도 생장이 왕성하여 재배가 쉽다 는 것이 가장 큰 장점이다. 본 연구는 펄프용 섬유로서 양호한 기본특성을 가진 신서란 을 이용한 펄프를 기존 제지공정에 일정량 투입함으로써 얻고자 하는 종이의 일반물성 개선 및 특수기능 향상에 도움이 되는 제지용 펄프로서의 가능성을 알아보고자 하였다.이어서 몇십%의 현을 억제하지는 못한다는 결론을 도출할 수 있었다. 연구의 결과를 요약하면 다음과 같다. 첫째, 한국과 미국 패션제품의 지난 십년간의 가격 과 품질의 상관관계는 매우 약한 정(+)의 상관을 가지고 있었다. 한국패션제품의 전체 서열상관계수의 평균은 0.091이었고, 미국의 상관계수 평균은 0.192였 다. 상관계수의 수치만으로 볼 때 한국보다는 미국 패션제품의 가격과 품질간의 상관관계가 조금 높게 나타났으나, 양국간 통계적인 차이는 없었다. 또한, 한국 패션제품의 경우에는 제품군의 가격대별 상관관계를 살펴보았는데, 그 결과 1만원 미만의 저가 제품군과 330만원이상의 고가의 제품군의 경우 부(-)의 상관계수를 나타냈다. 둘째. 1990년대 한국과 미국 패션시장의 가격과 품질간의 상관계수의 범위는 제품군별. 산업범주별 로 상이한 분포를 보이고 있었다. 패션제품군별로 보 면, 한국의 경우는 가장 높은 '여행용가방(r = 0.707)' 에서 가장 낮은 '자외선 차단화장품(r = -0.58)'까지, 그리고 미국 패션제품군의 상관계수의 범위는 '팬티 스타킹'의 0.820에서 '남성용 런닝슈즈'의 -0.472까지의 분포를 나타냈다. 마지막으로, 제품의 가격과 품질에 대한 정보를 소비자가 알 경우 얻을 수 있는 소비자의 구매이득 을 추산한 결과 패션제품시장에서 완전한 품질정보를 가지고 있다고 가정한 '현명한 사람'은, 최고가격의 제품만을 구입한다고 가정한 '부유한 사람들'의 구입방식에 비해 최고 좋은 품질의 상품을 총 지불액의 비율로 보아, 한국의 소비자는 약 50% 정도를, 미국의 소비자는 약 91%나 되는 액수를 절약하여 구매할 수 있는 것으로 나타났다. 이상의 본 연구의 결과를 종합하여 볼 때 한국과 미국 모두 제품군별, 그리고 산업범주별로 상관관계가 차이를 보이고 있어, 양국의 소비자들이 패션제품 의 품질을 평가함에 있어 오로지 가격에 대한