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http://dx.doi.org/10.15230/SCSK.2014.40.3.297

Cell migration and Anti-inflammatory Effect of Red Ginseng Extracts Fermented with Laetiporus Sulphureus  

Oh, Seong-Hwa (R&D Center, CoSeedBioPharm Corporation)
Choi, Soo-Yeon (R&D Center, CoSeedBioPharm Corporation)
Lee, Nu Rim (R&D Center, CoSeedBioPharm Corporation)
Lee, Jung No (R&D Center, CoSeedBioPharm Corporation)
Kim, Dong-Seok (Department of Food Service Industry, Seowon University)
Lee, Sang-Hwa (Department of Food Service Industry and Bio Organic Material & Food Center, Seowon University)
Park, Sung-Min (R&D Center, CoSeedBioPharm Corporation)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.3, 2014 , pp. 297-305 More about this Journal
Abstract
Red ginseng (RG) contains specific ginsenosides (Rg2, Rg3) which show various pharmacological effects and absorption rate in the body better than panax ginseng. Therefore many people have been used it for health for a long time. Furthermore, many researchers have been studying its biological activities for a long times because fermentation generates lots of beneficial small molecules good for health. In this study, we fermented red ginseng with mycelium of Leatiporus sulphures var. miniatus for 7 days. As a result, we found that three ginsenosides Rg1, Re and Rb2 were decreased from 0.24, 0.25, 0.16 mg/g to 0.12, 0.1, 0.03 mg/g respectively HPLC analysis. In addition, we studied biological activities of fermented red ginseng (FRG) about skin ageing such as anti-inflammation, cell migration, anti-oxidation, collagen type 1 synthesis, and MMP-1 inhibition activities. As a result, FRG were shown higher anti-inflammatory and cell migration promoting activities than RG. FRG inhibited production of nitric oxide (NO) and mRNA expression of inducible nitric oxide synthase (iNOS) and decreased interleukin (IL)-6 induced by LPS stimulation in RAW 264.7 cells. In conclusion, this study suggest that FRG could be a potential source as a new natural anti-inflammatory agent.
Keywords
Laetiporus sulphureus var. miniatus; anti-inflammatory; saponin; ${\beta}$-glucan; Fermented red ginseng;
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Times Cited By KSCI : 13  (Citation Analysis)
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1 H. D. Hong, S. Y. Choi, Y. C. Kim, Y. C. Lee, and C. W. Cho, Rapid determination of ginsenosides Rb1, Rf, and Rg1 in korean ginseng using HPLC, J. Ginseng Res., 33(1), 8 (2009).   DOI
2 K. Y. Nam, The comparative understanding between red ginseng and white ginsengs, processed ginsengs (Panax ginseng C. A. Meyer), J. Ginseng Res., 29(1), 1 (2005).   과학기술학회마을   DOI
3 K. S. Choi, H. Song, E. H. Kim, J. H. Choi, H. Hong, Y. M. Han, and K. B. Hahm, Inhibition of hydrogen sulfide-induced angiogenesis and inflammation in vascular endothelial cells: potential mechanisms of gastric cancer prevention by korean red ginseng, J. Ginseng Res., 36(2), 135 (2012).   과학기술학회마을   DOI   ScienceOn
4 J. Zhu, C. Luo, P. Wang, Q. He, J. Zhou, and H. Peng, Saikosaponin A mediates the inflammatory response by inhibiting the MAPK and NF-$\kappa$B pathways in LPS-stimulated RAW 264.7 cells, Eeperimental and Therapeutic Medicine, 5(5), 1345 (2013).   DOI
5 E. H. Sohn, S. A. Jang, C. H. Lee, K. H. Jang, S. C. Kang, H. J. Park, and S. Pyo, Effects of korean red ginseng extract for the treatment of atopic dermatitis-like skin lesions in mice, J. Ginseng Res., 35(4), 479 (2011).   과학기술학회마을   DOI   ScienceOn
6 Y. S. Kim, I. H. Cho, M. J. Jeong, S. J. Jeong, S. Y. Nah, Y. S. Cho, S. H. Kim, A. Go, S. E. Kim, S. S. Kang, C. J. Moon, J. C. Kim, S. H. Kim, and C. S. Bae, Therapeutic effect of total ginseng saponin on skin wound healing, J. Ginseng Res., 35(3), 360 (2011).   과학기술학회마을   DOI   ScienceOn
7 B. H. Han, M. K. Park, and E. S. Lee, Studies on the absorption and exretion of ginsenosieds, Kor. J. Ginseng Sci., 15(2), 112 (1991).
8 S. J. Park, D. H. Kim, N. S. Paek, and S. S. Kim, Preparation and quality characteristics of the fermentation product of ginseng by lactic acid bacteria (FGL), J. Ginseng res., 30(2), 88 (2006).   과학기술학회마을   DOI
9 S. H. Bae, H. S. Lee, M. R. Kim, S. Y. Kim, J. M. Kim, and H. J. Suh, Changes of ginsenoside content by mushroom mycelial fermentation in red ginseng extract, J. Ginseng Res., 35(2), 235 (2011).   과학기술학회마을   DOI   ScienceOn
10 X. Xu, M. Yasuda, S. N.Tsuruta, M. Mizuno, and H. Ashida, Glucan from lentinus edodes inhibits nitric oxide and tumor necrosis factor. Production and phosphorylation of mitogen-activated protein kinases in lipopolysaccharidestimulated murine RAW 264.7 macrophages, J. Biol. Chem., 287(2), 871 (2012).   DOI
11 D. Akramiene, A. Kondrotas, J. Didziapetriene, and E. Kevelaitis, Effects of b-glucans on the immune system, Medicina Kaunas, 43(8), 597 (2007).
12 M. Y. Lung and W. Z. Huang, Antioxidant properties of polysaccharides from Laetiporus sulphureus in submerged cultures, Afr. J. Biotechnol., 11(23), 6350 (2012).
13 M. R. Hong, Y. S. Kim, A. R. Joo, J. K. Lee, Y. S. Kim, and D. K. Oh, Purification and characterization of a thermostable beta-1,3-1,4-glucanase from Laetiporus sulphureus var. miniatus, J. Microbiol. Biotechnol., 19(8), 818 (2009).   과학기술학회마을   DOI   ScienceOn
14 J. Chen and R. Seviour, Medicinal importance of fungal b-(1/3), (1/6)-glucans, Mycological Research, 111(6), 635 (2007).   DOI   ScienceOn
15 T. Yayeh, K. H. Jung, H. Y. Jeong, J. H. Park, Y. B. Song, Y. S. Kwak, H. S. Kang, J. Y. Cho, J. W. Oh, S. K. Kim, and M. H. Rhee, Korean red ginseng saponin fraction downregulates proinfl ammatory mediators in LPS stimulated RAW264.7 cells and protects mice against endotoxic shock, J. Ginseng Res., 36(3), 263 (2012).   DOI   ScienceOn
16 D. H. Kim, J. H. Chung, J. S. Yoon, Y. M. Ha, S. J. Bae, E. K. Lee, K. J. Jung, M. S. Kim, Y. J. Kim, M. K. Kim, and H. Y. Chung, Ginsenoside Rd inhibits the expressions of iNOS and COX-2 by suppressing NF-kB in LPS-stimulated RAW264.7 cells and mouse liver, J. Ginseng Res., 37(1), 54 (2013).   DOI
17 S. I. Hwang, H. J. Ju, H. D. Kim, T. W. Kwak, B. H. Yoo, S. G. Kim, C. W. Lee, T. H. Hwang, and W. G. An, Anti-inflammatory effect of fermented red ginseng via regulation of NF-$\kappa$B signal transduction, Kor. J. Ori. Med. Physiol. Pathol., 25(3), 521 (2011).   과학기술학회마을
18 K. C. Song, T. S. Chang, H. J. Lee, J. H. Kim, J. H. Park, and G. S. Hwang, Processed panax ginseng, sun ginseng increases type I collagen by regulating MMP-1 and TIMP-1 expression in human dermal fibroblasts, J. Ginseng Res., 36(1), 61 (2012).   과학기술학회마을   DOI   ScienceOn
19 H. S. Lee, M. R. Kim, Y. H. Park, H. J. Park, U. J. Chang, S. Y. Kim, and H. J. Suh, Fermenting red ginseng enhances its safety and efficacy as a novel skin care anti-aging ingredient : In vitro and animal study, J. Med. Food, 15(11), 1015 (2012).   과학기술학회마을   DOI   ScienceOn
20 Y. C. Chen, Y. Sugiyama, N. Abe, R. Kuruto-Niwa, R. Nozawa, and A. Hirota, DPPH radical-scavenging compounds from dou-chi, a soybean fermented food, Biosci. Biotechnol. Biochem., 69(5), 999 (2005).   DOI   ScienceOn
21 M. Xue and C. J. Jackson, Autocrine actions of matrix metalloproteinase (MMP)-2 counter the effects of MMP-9 to promote survival and prevent terminal differentiation of cultured human keratinocytes, J. Invest. Dermatol., 128(11), 2676 (2008).   DOI   ScienceOn