• Title/Summary/Keyword: 자구 형성

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Effective and Economical Propagation Method of Lycoris squamigera Native to Korea (한국산 자생 상사화(Lycoris squamigera MAX.)의 효과적인 번식방법)

  • Park, Yun Jum;Heo, Buk Gu;Jeong, So Young;Jeong, Jai Ho;An, Min Sil
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.242-243
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    • 1998
  • This research was conducted to find the most effective and economical artificial propagation method of Lycoris squamigera among chipping, half-chipping, twin-scaling, coring, scooping and notching, in order to investigate the number of bulblets obtained from one bulb and the number of bulbs treated by a man in a day for artificial propagation. We got 42.2 bulblets from one bulb by twin-scaling, 23.2 bulblets by half-chipping, 18.2 bulblets by chipping, 14.3 bulblets by notching, 1 bulblet by coring and also 1 bulblet by scooping. Although we got the most bulblets by twin-scaling, it took the most time to treat the bulbs for artificial propagation (200 bulbs per day) and the bulblets formed were very small. But 400 bulbs were treated in a day by chipping and the bulblets formed were comparatively large. In addition, machines could be used for chipping, so chipping was considered to be the most effective and economical artificial propagation method.

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Establishment of in Vitro Culture and Effect of Chilling Treatment of Mother Bulb On Bulblet Formation in Fritillaria thunbergii Miq. (패모의 초기배양과 모구의 저온처리가 자구형성에 미치는 영향)

  • Yu, Kwang-Jin;Paek, Kee-Yeoup;Seong, Nak-Sul;Choi, In-Sick;Cho, Jin-Tae
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.3
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    • pp.211-218
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    • 1994
  • This experiment was conducted to obtain basic information for the establishment of in vitro initial culture system in Fritillaria thuubergii Miq. Methods of surface sterilization of scale segments as explant and effect of antibiotics added into the culture medium on contamination of explant and chilling treatment of mother bulb on bulblet formation were investigated. Portent of contamination of cultured scale segments was significantly higher in the outer scale segments which were unsuitable as initial culture explant than inner scale segments. Contamination of explants taken from inner scale of bulb was reduced by surface sterilizing explants in the solution of $4{\sim}5%$ sodium hypoclorite for $10{\sim}15$ mimutes. Addition of antibiotics such as kanamycin, vancomycia cefotaxim, agrirnycin and agreptomycin and dithane as fungicide and$lncyte^{tm}$ into MS medium was effective to reduce bateriological contamination, but did not work to control fungi. It had effective to delay the degree of contamination caused by fungi and bacteria haboring in cultured explants. Bulblet formation from cultured scale segments was promoted by dry storage for $2{\sim}4$ weeks or moisture storage of mother bulbs for $4{\sim}6$ weeks at $10^{\circ}C$ before excision of explants. Addition of kinetin into medium could not exerted for the bulblet formation from the scale segment of dry storaged bulb compared to control. But explant taken from 6 week moisture storaged bulb formed more than 10 bulblets per explant on the medium containing $3{\sim}5mg/L$ kinetin.

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Cultural Environments on Growth and Tuberlet Yield of Pinellia ternata(Thunb.) Breit (반하의 재배환경에 따른 생육 및 수량)

  • Kim, Young-Jin;Park, Moon-Soo;Park, Ho-Ki;Kim, Sun;Kim, Tai-Soo;Chang, Young-Sun
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.3
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    • pp.240-245
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    • 1995
  • This study was conducted to find out environment conditions in order to increase of growth and yield of tuberlets in pinellia ternata (Thunb.) Breit. The optimal temperature conditions for the growth of tuberlets were good rather temperature fluctuations than constant one and the optimal photoperiod was a 6hr-photoperiod. The growth of tuberlets to the kinds and concentration of growth regulators was good for 0. lppm 2.4- D, 10ppm kinetin and 50ppm gibberellin treatment. In colchicine soaking treatment, the growth of tuberlets was bad, but bulblets formation was good for 0. 05% colchicine treatment for 48hr. -soaking. In colchicine dropping treatment, dry tuber yield was good for 0. 1% colchicine treatment with once dropping for one day.

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The Magnetic Properties of FeBSiNb Alloy Ribbons with High Glass forming Ability (고 비정질 형성능을 가진 FeBSiNb 합금 리본의 자기적 특성)

  • Noh, Tae-Hwan;Kim, Gu-Hyun
    • Journal of the Korean Magnetics Society
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    • v.12 no.4
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    • pp.154-159
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    • 2002
  • Amorphous FeBSiNb alloy ribbons having bulk glass forming ability and high saturation magnetic flux density were produced by single-roller melt spinning apparatus in the thickness range of 22∼102㎛. With the increase of thickness, the coercive force and squareness ratio decreased, while maximum permeability and AC permeability increased. However electrical resistivity was almost constant. Furthermore refined and complex magnetic domain structure was observed in thicker ribbons owing to the change in internal magnetic anisotropy. For the alloy with the thickness of 81㎛, small coercive force of 24 mOe and high effective permeability of 12,000 at 1㎑ were obtained, those are considered to be better comparing to the conventional soft magnetic amorphous alloys (∼20 ㎛). The good soft magnetic properties of the thick FeBSiNb amorphous alloys were attributed to the decrease in surface pinning effect during wall motion, appearance of perpendicular anisotropy and resulted domain refinement.

Effect of MS Medium Strength, Sucrose Concentration, and Light Condition on Bulblet Formation and Growth of Muscari armenicum In Vitro (MS 배지내 무기물 농도, 당 농도 및 광 조건이 무스카리의 기내 자구 형성과 비대에 미치는 영향)

  • Chung, Mi-Young;Kim, Chang-Kil;Chung, Jae-Dong
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.49-54
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    • 2011
  • The influences of MS medium strength, sucrose concentration, and light condition on bulblet formation and growth were studied in leaf tissue culture of Muscari armenicum 'Early Giant'. Bulblet formation from leaf segments were the most effective on MS medium supplemented with $0.01mg{\cdot}L^{-1}$ NAA, $0.2m{\cdot}L^{-1}$ kinetin, $30g{\cdot}L^{-1}$ sucrose, and $8g{\cdot}L^{-1}$ gelrite under darkness for 2 weeks followed by 16 hr photoperiod with a photosynthetic photon flux density of $50mol{\cdot}m^{-2}{\cdot}s^{-1}$. However the compactness of bulblets formed in vitro was promoted in the MS medium with $60gL^{-1}$ sucrose. Acclimatized plants flowered during the second year of the growing period without any phenotypic variations and formed average 1.5 bulblets per mother bulb.

In Vitro Propagation of Narcissus pseudonarcissus by Scale Cultures Using Thidiazuron (인편의 Thidiazuron처리에 의한 나팔수선의 기내증식)

  • 이병기;김영숙;박병모
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.1
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    • pp.53-57
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    • 1995
  • This study was carried out to investigate the effect of TDZ (thidiazuron) treatment on Propagation in vivo through scale cultures of Narcissus pseudonarcissus. Scales with disk (5 to 7 m in size) were cultured on MS medium containing NAA, BA and TDZ. Bulbs and shoots were directly formed when scales were cultured on medium containing 5 mg/L NAA and 1 mg/L BA. In addition, combination of 3 mg/L NAA and 0.02 mg/L TDZ promoted effectively the direct formation of bulbs and shoots. The shoots were rooted when cultured on medium containing 5 mg/L NAA and 0.02 mg/L TDZ. non scales obtained from regenerates were cultured on medium containing 1.0 mg/L NAA and 0.5 mg/L TDZ, they gave rise to numerous bulbs and shoots . The overall results suggest that TDZ is an effective plant growth regulator in vitro propagation of N. pseudonarcissus.

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Plant Regeneration from Immature Embryo and Bulb Scale Tissue of Hippeastrum hybridum (아마릴리스의 미숙배와 인편조직으로부터 식물체 재분화)

  • 최은경;박학봉
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.1
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    • pp.27-31
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    • 1998
  • Immature hybrid embryos of H. hybridum, 'Picottee', 'White Christmas', 'Eldorado', 'Origin', 'Red Lion', 'elstar', 'Crypsy' were cultured on the MS medium supplemented with various concentrations of 2,4-D, NAA, BA and TDZ. Among the treatments, NAA were more effective for the shoot regeneration and bulblet formation than other treatment. Addition of 0.5 ㎎/L NAA was effective for bulblet induction from explant Shoot regeneration was most effective on the medium with 1.0㎎/L NAA and 2.0 ㎎/L TDZ. The addition of 1.0-2.0㎎/L TDZ induced numerous shoots per explant but strongly inhibited root development when compared to 1.0-2.0㎎/L BA. When bulb scale segments of 'Star Van Holland' was incubated, bulblet formation was the most effective on MS medium with 0.5㎎/L NAA.

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Micromagnetic Analysis of Thermal Magnetization Fluctuations in Ferromagnetic Nanowires (미세자기 동역학을 이용한 강자성 나노선의 자기 잡음 연구)

  • Yoon, Jung-Bum;You, Chun-Yeol;Jo, Young-Hun;Park, Seung-Young;Jung, Myung-Hwa
    • Journal of the Korean Magnetics Society
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    • v.20 no.1
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    • pp.1-7
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    • 2010
  • We investigate the spin dynamics of the magnetic domain wall using the magnetic noise in the magnetic nanowire structure by employing micromagnetic simulations. Magnetic noise due to the thermal fluctuations in ferromagnetic materials is related to magnetic susceptibility and resonance frequency, which are important physical quantities in the study of the spin dynamics. In this study, we present the magnetic noise of the single domain without magnetic domain wall, and with the magnetic domain wall between two magnetic domains in ferromagnetic nanowires. It is confirmed that the Kittel equation with simple ellipsoid model with demagnetizing factor well describe the resonance frequency due to magnetic noise of the single domain. Besides, we find that there is a distinguishable additional resonance frequency, when a magnetic domain wall exists. It is verified that the additional resonance frequency is originated from the magnetic domain wall, and it is lower than one of the single domain. It implies that the spins inside the domain wall have a different effective field.

Effect of Irradiation on Growth, Bulblet Formation, and Germination of Pollen and Seed of Several Lily Cultivars (나리 생장, 자구 발달 및 화분과 종자발아에 미치는 방사선의 영향)

  • Park, In Sook;Suh, Dong Hee;Hwang, Yoon Jung;Chung, Jae-Dong;Kang, Si-Yong;Lim, Ki-Byung
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.211-217
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    • 2008
  • To build up data for mutation breeding of lily, sensitivity to irradiation of bulb, seed and pollen was investigated. The bulbs of seven cultivars including 'New Wave' were very sensitive to gamma rays. Non-irradiated bulbs produced plants with bloom as normal. However, irradiated bulbs showed retarded growth and did not flower. Bulbs of 'New Wave' and 'Tiny Dino' irradiated with 125Gy gamma rays survived, but their growth rate dropped considerably. Part or whole leaves of the other cultivars except 'New Wave' and 'Tiny Dino' withered. Moisture content of individual scale was 72~78% depending on cultivar. Bulblet formation from untreated scales was 100%. Among all irradiated scales only 'Siberia' scales showed 8.5% bulblet formation when irradiated with 50Gy gamma rays. Pollen germination as affected by gamma ray irradiation had no consistent tendency. When dosage of $F_1$ 'Augusta' seeds reached 300Gy, their survival ratio was drastically decreased. Bulblet formation in vitro was observed only in non-irradiated seeds. Lethal dose ($LD_{50}$) of seed was less than 100Gy. Height and width of $F_1$ 'Augusta' bulblets in vitro irradiated with gamma rays decreased as dosage increased regardless of the culture method. The percentage shoot formation was 100% in non-irradiated bulblets and 15~60% in irradiated ones.

Effect of Explant and Cultivars on the Adventitious Shoot Differentiation by Invitro Culture of Narcissus (배양재료와 품종이 수선의 기내배양시 부정아 형성에 미치는 영향)

  • 정향영;한봉희
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.2
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    • pp.103-106
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    • 1997
  • In order to establish a micropropagation system of Naricissus, the ability of bulblet regeneration among propagation materials was compared, and the adequate growth regulators and concentrations for each cultivar were investigated. The inorganic components were also assayed in the parts of propagation materials. In propagation materials, scape with based plates showed hightest rate of bulblet formation and rapid growth of formed bulblets in vitro, comparing to other parts of it. In comparing of varieties, 'Dutch Master' and 'Golden Harvest' showed a high ability for bulblet regeneration. The ability of bulblet regeneration was most favorable in the medium, supplemented with 5.0 mg/L BA and 2.5 mg/L NAA in 'Dutch Master', and 5.0 mg/L BA and 1.0mg/L NAA in 'Golden Harvest', respectively. In inorganic component analysis of propagation materials, the White part of scape contained 1.18 mg/L$P_2O_5$, 2.57 me Ca, 0.94 me Mg and 3.20 mg/L total N. It showed higher levels in concentration of inorganic components as compared to those of the other part of scape. In addition, leaves and yellow part of scape contained significantly high levels of Ca and Mg while scales bulb showed considerably low levels in all inorganic compounds.

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