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Effect of MS Medium Strength, Sucrose Concentration, and Light Condition on Bulblet Formation and Growth of Muscari armenicum In Vitro  

Chung, Mi-Young (Department of Horticultural Sciences, Kyungpook National University)
Kim, Chang-Kil (Department of Horticultural Sciences, Kyungpook National University)
Chung, Jae-Dong (Department of Horticultural Sciences, Kyungpook National University)
Publication Information
FLOWER RESEARCH JOURNAL / v.19, no.1, 2011 , pp. 49-54 More about this Journal
Abstract
The influences of MS medium strength, sucrose concentration, and light condition on bulblet formation and growth were studied in leaf tissue culture of Muscari armenicum 'Early Giant'. Bulblet formation from leaf segments were the most effective on MS medium supplemented with $0.01mg{\cdot}L^{-1}$ NAA, $0.2m{\cdot}L^{-1}$ kinetin, $30g{\cdot}L^{-1}$ sucrose, and $8g{\cdot}L^{-1}$ gelrite under darkness for 2 weeks followed by 16 hr photoperiod with a photosynthetic photon flux density of $50mol{\cdot}m^{-2}{\cdot}s^{-1}$. However the compactness of bulblets formed in vitro was promoted in the MS medium with $60gL^{-1}$ sucrose. Acclimatized plants flowered during the second year of the growing period without any phenotypic variations and formed average 1.5 bulblets per mother bulb.
Keywords
NAA; kinetin; sucrose; photoperiod;
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