Abstract
In order to establish a micropropagation system of Naricissus, the ability of bulblet regeneration among propagation materials was compared, and the adequate growth regulators and concentrations for each cultivar were investigated. The inorganic components were also assayed in the parts of propagation materials. In propagation materials, scape with based plates showed hightest rate of bulblet formation and rapid growth of formed bulblets in vitro, comparing to other parts of it. In comparing of varieties, 'Dutch Master' and 'Golden Harvest' showed a high ability for bulblet regeneration. The ability of bulblet regeneration was most favorable in the medium, supplemented with 5.0 mg/L BA and 2.5 mg/L NAA in 'Dutch Master', and 5.0 mg/L BA and 1.0mg/L NAA in 'Golden Harvest', respectively. In inorganic component analysis of propagation materials, the White part of scape contained 1.18 mg/L$P_2O_5$, 2.57 me Ca, 0.94 me Mg and 3.20 mg/L total N. It showed higher levels in concentration of inorganic components as compared to those of the other part of scape. In addition, leaves and yellow part of scape contained significantly high levels of Ca and Mg while scales bulb showed considerably low levels in all inorganic compounds.
수선의 기내대량 번식방법을 확립시키기 위해 배양재료별 증식능력과 품종별 적정 생장조절제 농도 및 분화능력을 구명하고 아울러 배양재료간의 무기성분을 분석하였다. 배양재료별로는 화경에 기저부를 부착한 것이 자구형성율이 75%로 가장 높았고 기내에서의 자구생육도 양호하였다. 품종간에는 'Dutch Master'와 'Golden Harvest'가 증식력이 가장 높았고 'Dutch Master'는 BA 5 mg/L + NAA 2.5 mg/L 'Golden Harvest'는 BA 5mg/L + NAA 1 mg/L을 첨가한 배지에서 자구증식능력이 가장 좋았다. 배양재료별 무기성분은 화경의 백색부위가 P$_2$O$_{5}$ 1.18 mg/L, K 8.71 me, Ca 2.57 me, Mg 0.94 me, T-N 3.20 mg/L으로 다른 부위에 비해 고루 많은 양을 함유하고 있었으며 Ca, Mg은 엽과 화경의 황색부위에 많이 함유되어 있었고 인편은 저농도의 무기성 분을 함유하고 있었다.
