FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
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Effect of Irradiation on Growth, Bulblet Formation, and Germination of Pollen and Seed of Several Lily Cultivars

나리 생장, 자구 발달 및 화분과 종자발아에 미치는 방사선의 영향

  • Park, In Sook (School of Applied Bioscience, Kyungpook National University) ;
  • Suh, Dong Hee (School of Applied Bioscience, Kyungpook National University) ;
  • Hwang, Yoon Jung (School of Applied Bioscience, Kyungpook National University) ;
  • Chung, Jae-Dong (School of Applied Bioscience, Kyungpook National University) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, KAERI) ;
  • Lim, Ki-Byung (School of Applied Bioscience, Kyungpook National University)
  • 박인숙 (경북대학교 응용생명과학부) ;
  • 서동희 (경북대학교 응용생명과학부) ;
  • 황윤정 (경북대학교 응용생명과학부) ;
  • 정재동 (경북대학교 응용생명과학부) ;
  • 강시용 (한국원자력연구원 방사선과학연구소) ;
  • 임기병 (경북대학교 응용생명과학부)
  • Received : 2008.04.02
  • Accepted : 2008.09.22
  • Published : 2008.09.30

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Abstract

To build up data for mutation breeding of lily, sensitivity to irradiation of bulb, seed and pollen was investigated. The bulbs of seven cultivars including 'New Wave' were very sensitive to gamma rays. Non-irradiated bulbs produced plants with bloom as normal. However, irradiated bulbs showed retarded growth and did not flower. Bulbs of 'New Wave' and 'Tiny Dino' irradiated with 125Gy gamma rays survived, but their growth rate dropped considerably. Part or whole leaves of the other cultivars except 'New Wave' and 'Tiny Dino' withered. Moisture content of individual scale was 72~78% depending on cultivar. Bulblet formation from untreated scales was 100%. Among all irradiated scales only 'Siberia' scales showed 8.5% bulblet formation when irradiated with 50Gy gamma rays. Pollen germination as affected by gamma ray irradiation had no consistent tendency. When dosage of $F_1$ 'Augusta' seeds reached 300Gy, their survival ratio was drastically decreased. Bulblet formation in vitro was observed only in non-irradiated seeds. Lethal dose ($LD_{50}$) of seed was less than 100Gy. Height and width of $F_1$ 'Augusta' bulblets in vitro irradiated with gamma rays decreased as dosage increased regardless of the culture method. The percentage shoot formation was 100% in non-irradiated bulblets and 15~60% in irradiated ones.

나리 돌연변이 육종을 위한 기초자료로서 구근, 종자, 화분의 방사선 감수성을 구명하고자 하였다. 'New Wave'외 6품종의 구근은 감마선에 매우 민감하였다. 무조사구는 개화하였으나 조사구에서는 생육이 크게 지연되었으며 화서는 전혀 형성되지 않았다. 'New Wave'와 'Tiny Dino'는 125Gy 조사구에서 생존은 하였으나 생육은 저조하였다. 'New Wave'와 'Tiny Dino'를 제외한 다른 품종들은 잎의 일부 또는 전부가 고사하였다. 품종별 인편의 수분함량은 72~78%이었다. 인편으로부터 자구 형성은 무조사구에서 100%, 조사된 구에서는 'Siberia' 50Gy 처리구에서만 8.5% 일어났다. 화분의 발아율은 감마선 조사에 의해 일정한 경향을 나타내지 않았다. $F_1$ 'Augusta' 종자는 선량이 높아질수록 생존율은 급격히 떨어졌다. 자구는 무조사구에서만 형성되었으며 $LD_{50}$는 100Gy 미만이었다. 방사선 조사된 기내 $F_1$ 'Augusta'의 자구는 배양방법에 관계없이 구고와 구폭이 선량 증가와 함께 감소하였다. 신초 형성은 무조사구에서 100% 되었으나 다른 처리구에서는 15~16%로 떨어졌다.

Keywords

Acknowledgement

Supported by : 농촌진흥청