• Korean Chemical Engineering Research
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• v.49 no.6
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• pp.840-845
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• 2011

Degradation products of agarose are biologically active and thus used as an ingredient in pharmaceuticals or functional cosmetics. Furthermore, it has been strongly considered as a substrate for bio-ethanol fermentation. Recently, we isolated new agarase-producing microorganism, Pseudoalteromonas sp. from south sea of Korea. In this study, we aimed to separate and purify the agarase from culture broth of this strain. Separation of agarase was performed by ion- exchange chromatography on DEAE-Sepharose resin. Equilibrium pH and volume ratio of resin to the amount of protein were optimized for the efficient adsorption of protein. 410 ${\mu}g$ of protein was completely adsorbed to 3 mL of resin at pH 7.5. The total amount of eluted protein increased as NaCl concentration increased to 400 mM at isocratic elution. Agarase was separated by linear gradient elution of NaCl (0~1,000 mM). Three major protein peaks were observed and the presence or absence of agarase in these eluted proteins was measured by Lugol's staining technique. Only six eluted protein fractions showed strong agarase activity.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.11
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• pp.855-859
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• 2011

The main pollutants from zirconium alloy tubing manufacturing process in nuclear industry are nitrate ($NO_3-N$) and fluoride (F-)Nitric acid, and hydrofluoric acid is used for acid pickling. The process for the removal of nitrate and fluoride is composed of 1st chemical coagulation, SOD (Sulfur Oxidation Denitrification) process using sulfur-oxidizing denitrification, and 2nd chemical coagulation. The characteristic of the wastewater treatment is an application of SOD process. The SOD Process is highly received attention because it is significantly different from existing processes for sulfur denitrification. A JSC (JeonTech-Sulfur- Calcium) Pellet is unification of sulfur and alkalinity material. According to result of SOD process in wastewater treatment plant, the removal efficiency of T-N was over 91% and the average concentration of T-N from influent was 147.55 mg T-N/L and that from effluent was 12.72 mg T-N/L. Therefore, SOD process is a useful to remove nitrogen from inorganic industrial wastewater and a new development of microbial activator was shown to be stable for activation of autotrophic bacteria.

• KSBB Journal
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• v.31 no.4
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• pp.263-269
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• 2016

For decades, the microorganisms in arctic soils have been newly discovered according to the climate change and global warming. In this study, the chemical structure of a lipid A molecule from Pseudomonas sp. strain PAMC 28615 which was newly discovered from arctic soils was characterized by mass spectrometric approaches such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI multi-stage tandem mass spectrometry (MS). First, lipopolysaccharide (LPS) from Pseudomonas sp. strain PAMC 28615 was extracted and subsequently hydrolyzed to obtain the lipid A. The parent ion peak at m/z 1632 was determined by MALDI-TOF MS, which also can validate our lipid A purification method. For detailed structural determination, we performed the multiple-stage tandem mass analysis ($MS^4$) of the parent ion, and subsequently the abundant fragment ions in each MS stage are tested. The fragment ions in each MS stage were produced from the loss of phosphate groups and fatty acyl groups, which could be used to confirm the composition or the position of the lipid A components. Consequently, the mass spectrometry-based lipid A profiling method could provide the detail chemical structure of lipid A from the Pseudomonas sp. strain PAMC 28615 as an arctic bacterium from the frozen arctic soil.

• Research in Plant Disease
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• v.26 no.1
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• pp.48-52
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• 2020

In July 2019, typical rot symptom was observed on peach fruits harvested from the fields at Andong, Korea. As the disease progressed, white and purple colored mycelial mat developed on the surface of the infected fruits. A causal pathogen was isolated from the infected fruit and cultured on potato dextrose agar media for identification. Fungal colonies on potato dextrose agar produced 3 pigments, including purple, yellow, and white colors. The isolate incited fruit rot symptoms on artificially inoculated peach fruits, from which the same fungus was isolated, fulfilling Koch's postulates. Based on the morphological characteristics and sequence analysis of rDNA internal transcribed spacer, translation elongation factor 1-alpha, and β-tubulin, the causal agent of the disease was identified as Fusarium avenaceum. This study is the first report of fruit rot of peach fruits caused by Fusarium avenaceum in Korea.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2013.11a
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• pp.143-143
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• 2013

대학, 기업 및 국 공립 출연 연구소의 연구 활동 종사자는 학술적 연구 및 신기술, 신물질의 개발을 통해 우리나라 과학기술 발전에 크게 기여하고 있다. 그러나 이와 더불어 각종 안전사고에 노출되어 있는 것도 사실이다. 즉, 실험실은 대부분의 작업장 보다 더욱 많은 화재, 폭발 등의 위험에 항상 노출되어 있다. 이는 일반적인 작업장과는 다르게 실험이 종료되면 기존 실험 장치를 철거하고 신규로 다른 실험장치가 설치되기 때문이다. 연구실험실에서 발생한 사고 원인을 분석해 보면, 실험실 기능의 불비, 설비 및 유지관리의 불충분, 실험공간의 협소, 실험 작업 안전에 대한 배려부족, 안전전문가의 부족과 교육훈련체제의 미흡 등을 지적할 수 있으며, 이와 같은 빈번한 사고발생은 우수한 연구 활동 종사자의 연구 의욕을 떨어뜨리고, 건강과 생명에 위협을 느끼게 하고 있다. 본 연구에서는 기존의 실험실 안전관리 체크리스트들을 검토하고, 안전관련 분야에서 사용되고 있는 심의 체크리스트를 조사하여, 대학 및 연구기관 등의 바이오실험실에 사용할 수 있는 표준 체크리스트를 개발하고자 한다. 현재 개발된 바이오 분야 체크리스트는 "연구실험실 공통 체크리스트"에 기반하여 바이오 분야에서 중점 평가할 항목을 심화시켜 구성하였다. 바이오 분야에서 주로 이루어지는 생물 실험 및 동물해부 실험시 안전을 위해 반드시 점검해야 할 총 8개의 세부 점검 항목을 제시하였으며, 실험동물 관리 및 사육실 출입시 안전을 위해 점검해야 할 총 11개의 세부 점검 항목을 제시하였고, 세포, 조직 배양 실험 및 유전자 조작 실험시 안전을 위해 점검해야 할 총 5개의 세부 점검 항목을 제시하였다. 또한 미생물 취급시 안전을 위해 점검해야 할 총 19개의 세부 점검 항목을 제시하였으며, 세균 취급시 안전을 위해 점검해야할 총 8개의 세부 점검 항목을 제시하였고 마지막으로, 바이러스 취급시 안전을 위해 점검해야할 총 5개의 세부 점검 항목을 제시하였다. 실험실 안전은 연구종사자의 올바른 안전의식, 연구관련 부서의 체계적 지원 및 실험실 안전 관련 법령의 완비가 뒷받침 되었을 때 비로소 가능한 일이다. 따라서, 본 연구에서 얻어진 체크 리스트가 이에 기여할 것이라 사료되며, 추후 분야별 실험실 안전관리 실태조사의 지침서로 활용되는데 목적이 있다.

• Journal of Food Hygiene and Safety
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• v.36 no.5
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• pp.440-446
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• 2021

GABA (γ-aminobutyric acid) has various beneficial effects on human health such as anti-hypertension, diuretic, tranquilizer, sleep induction and anti-stress functions. In this study, the properties and the antioxidizing effects of a fermented solution was investigated by applying GABA producing lactic acid bacteria (LAB) from kimchi to corn silk extract. Lactobacillus plantarum LAB459 was identified by physiological properties, carbohydrate fermentation pattern and 16s rRNA sequence analysis. Also, the GABA production ability of the separated L. plantarum LAB459 was confirmed through TLC and HPLC analysis. Moreover, from the fermentation of corn silk extract with skim milk, it was revealed that approximately 1 ㎍/mg of GABA produced by lyophilized ferments was yielded. Lastly, the flavonoid content and DPPH radical scavenging activity were found to be high in the lyophilized ferments than in the aqueous extracts. Therefore, L. plantarum LAB459 is considered to be used as a starter culture for various fermented foods or in food and medicinal materials.

• Journal of Life Science
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• v.20 no.2
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• pp.269-274
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• 2010

An antifungal antibiotic-producing strain, BCNU 2003, was isolated from forest soil in Korea. The morphological and physiological characters, and 16S rRNA sequences analysis of strain BCNU 2003 identified this strain as Bacillus genus. The Bacillus sp. BCNU 2003 showed strong antifungal activities against Aspergillus niger, Trichophyton mentagrophytes and Trichophyton rubrum with inhibition ranging from 62.05 to 63.49% by using dual culture technique. Bacillus sp. BCNU 2003 produced a maximum level of antifungal substances under aerobic incubation at 28oC and pH 6.5-7.2 for 6 days in LB broth. Ethyl acetate extract of the cultured broth showed strong antifungal activity and a broad antifungal spectrum against various pathogenic fungi. The minimum inhibitory concentration (MIC) values for its active extracts ranged between 0.0625 mg/ml and 1 mg/ml. In addition, Bacillus sp. BCNU 2003 was determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.76-81
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• 2006

창녕군 증산리 지역은 신규 취수원 확보 일환으로 강변여과 방식 취수 가능성이 한국수자원공사에 의해서 평가되고 있는 지역이다. 강변여과 방식 취수의 타당성 평가를 위해 현장 지질조사, 시추조사, 전기 비저항 탐사 및 고해상도 탄성파 탐사를 수행하였다. 또한 대상 연구지역에서의 개략적 취수 가능량 산정을 위해 모델링 평가가 수행되었으며, 개별 정호에 대한 양수량 산정을 위해 시험 정호를 설치하였다. 현장 시추 조사와 시료에 대한 입도 분석 결과, 연구 지역의 충적층 두께는 35m 전후이며, 주 대수층 구간은 지표하 $25{\sim}35m$인 것으로 추정되었다. 또한 주 대수층 구간의 수리전도도는 $10^{-2}cm/sec$ 이상으로 주로 모래섞인 자갈층으로 구성되어있는 것으로 평가되었다. 또한 전기비저항 조사 결과는 부분적으로 매우 낮은 비저항 분포 지층을 보여주고 있으며, 이는 주로 실트 및 점토로 된 지층이 부분적으로 퇴적되어 있음을 지시하며, 고해상도 탄성파 탐사 결과는 전반적으로 지하수위는 지표하부 5m 전후에 분포하고, 충적층의 하부 경계는 35 내지 45m인 것으로 해석되었다. 지하수 모델링을 통해, 취수 목표량인 180,000톤/일은 주대수층까지의 지하수위 강하 없이 확보 가능할 것으로 평가되었다. 또한 개별 정호의 산출 특성을 평가하기 위해 시험 정호를 설치하여, 실제 2,700톤/일 예비 양수 시험을 수행하였다. 예비 양수 시험 결과, 양수정에서의 수위 강하는 개략 10m, 양수정관측정에서는 약 0.3m의 수위강하만이 관찰되었으며, 양수 영향권이 수 십 m를 넘지 않을 것으로 판단되었다.서의 S97과 JBR의 세포감염 억제율은 3.85%와 3.63%로 나타났다. $textsc{k}$-casein, CU는 로타바이러스 S97과 JBR에 대해 농도 2000UM에서 97%이상의 억제효과를 나타냈으며, sialic acid는 억제효과가 거의 없었다. K-casein, GMP는 송아지뿐만 아니라 유아의 로타바이러스에 의한 설사를 억제할 수 있을 것으로 기대된다.을 향상하기 위해서는 이러한 부위에 대한 미생물 오염을 낮출 수 있는 세심한 현장 품질관리가 필요하다.en and adolescents, analysed by country, age group and gender. The paper discusses the places young consumers can turn to in trying to fulfil their growing consumer needs. It also examines how much money is at their disposal. It then concludes by considering the influence of "financial socialization" on how young people deal with money.nsumption visions based on the various perspectives, consumers are influenced in the apparel buying decision-making. Many subjects reported experiencing positive affect when imagining positive outcomes of produc

• Journal of Life Science
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• v.21 no.4
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• pp.589-595
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• 2011

This work focused on screening and characterizing antibiotic-producing actinomycetes to develop new antibiotics that can overcome the growing resistance of disease-causing microbes. One-hundred actinomycetes strains were isolated from soil samples from Chungcheongbuk-do, Korea using various kinds of actinomycetes isolation media, including a starch casein agar medium and potato dextrose agar (PDA). Among them, strain BCNU 1030 was determined to show strong antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). Biochemical, physiological, and 16S rRNA sequence analyses indicated that strain BCNU 1030 belonged to the genus Streptomyces. Strain BCNU 1030 exhibited antibiotic activity against a wide range of bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of BCNU 1030 dichloromethane extract was determined to be $0.78\;{\mu}g/ml$ for MRSA CCARM 3090. Therefore, Streptomyces sp. BCNU 1030 has potential for anti-MRSA drug development.

• Journal of Life Science
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• v.27 no.4
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• pp.435-441
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• 2017

Perchlorate ($ClO_4^-$) is an emerging contaminant detected in soil, groundwater, and surface water. Previous study revealed bacterial community in the enrichment culture tdegraded perchlorate using elemental sulfur as an electron donor. Quantitative and qualitative molecular methods were employed in this study to investigate archaeal community in the enrichment culture. Real-time qPCR showed that archaeal 16S rRNA gene copy number in the culture was about 1.5% of bacterial 16S rRNA gene copy number. This suggested that less archaea were adapted to the environment of the enrichment culture and bacteria were dominant. DGGE banding pattern revealed that archaeal community profile of the enrichment culture was different from that of the activated sludge used as an inoculum for the enrichment culture. The most dominant DGGE band of the enrichment culture was affiliated with Methanococci. Further research is necessary to investigate metabolic role of the dominant archaeal population to better understand microbial community in the perchlorate-reducing enrichment culture.