Optimization of Anion-exchange Chromatography for the Separation of Agarase from Culture Broth of Pseudoalteromonas sp.
![]() |
Kim, Yu-Na
(Department of Chemical & Biological Engineering and ERI, Gyeongsang National University)
Lee, Jae-Ran (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) Kim, Mu-Chan (Department of Marine Environmental Engineering, Gyeongsang National University) Kim, Sung-Bae (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) Hong, Soon-Kwang (Division of Bioscience and Bioinformatics, Myong Ji University) Kim, Chang-Joon (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) |
1 | Lahaye, M. and Rochas, C., "Chemical Structure and Physicochemical Properties of Agar," Hydrobiol., 221, 137-148(1991). DOI ScienceOn |
2 | Michel, G., Nyval-Collen, P., Barbeyron, T., Czjzek, M. and Helbert, W., "Bioconversion of Red Seaweed Galactans: A Focus on Bacterial Agarases and Carrageenases," Appl. Microbiol. Biotechnol., 71, 23-33(2006). DOI ScienceOn |
3 | Ting, X. and Kim, S. M., "Agarase: Review of Major Sources, Categories, Purification Method, Enzyme Chracteristics and Applications," Mar. Drugs, 8, 200-218(2010). DOI ScienceOn |
4 | Flament, D., Barbeyron, T., Jam, M., Potin, P., Czjzek, M., Kloareg, B. and Michel, G., "Alpha-agarase Define a New Family of Glycoside Hydrolases, Distinct from Beta-agarase Families," Appl. Environ. Microbiol., 73(14), 4691-4694(2007). DOI ScienceOn |
5 | Ohta, Y., Hatada, Y., Miyazaki, M., Nogi, Y., Ito, S. and Horikoshi, K., "Purification and Characterization of a Novel a-agarase from a Thalassomonas sp.," Curr. Microbiol., 50, 212-216 (2005). DOI ScienceOn |
6 |
Potin, P., Richard, C., Rochas, C. and Kloareg, B., "Purification and Characterization of the |
7 |
Lakshmikanth, M., Manohar, S., Souche, Y. and Lalitha, J., "Extracellular |
8 |
Wang, J., Mou, H., Jiang, X. and Guan, H., "Characterization of a Novel |
9 |
Jam, M., Flament, D., Allouch, J., Potin, P., Thion, L., Kloareg, B., Czjzek, M., Helbert, W., Michel, G. and Barbeyron, T., "The Endo- |
10 |
Schroeder, D. C., Jaffer, M. A. and Coyne, V. E., "Investigation of the Role of a |
11 |
Hatada, Y., Ohta, Y. and Horikoshi, K., "Hyperproduction and Application of |
12 | Chen, H.-M., Zheng, L. and Yan, X.-J., "The Preparation and Bioactivity Research of Agaro-Oligosaccharides," Food Technol. Biotechnol. 43(1), 29-36(2005). |
13 | Shin, M. H., Lee, D. Y., Wohlgemuth, G., Choi, I.-G., Fiehn, O. and Kim, K. H., "Global Metabolite Profiling of Agarose Degradation by Saccharophagus degradans 2-40," New Biotechnol. 27(2), 156-168(2010). DOI ScienceOn |
14 | Lee, S.-M., Yu, B. J., Kim, Y. M., Choi, S.-J., Ha, J.-M., Lee, J.- H., "Production of Bio-ethanol from Agar using Saccharomyces cerevisiae," J. Kor. Ind. Eng. Chem. 20(3), 290-295(2009). |
15 | Lim, D.-J., Kim, B.-J., Bae, S.-K., Kim, J.-D. and Kong, J.-Y., "Immobilization of Agarase for the Agarooligosaccharide Production," Kor. J. Appl. Microbiol. Biotechnol. 27(3), 208-214 (1999). |
16 |
Lakshmikanth, M., Manohar, S. and Lalitha, J., "Purification and Characterization of |
17 | Sugano, Y., Terada, I., Arita, M., Noma, M. and Matsumoto, T., "Purification and Characterization of a New Agarase from a Marine Bacterium, Vibrio sp. Strain JT0107," Appl. Environ. Microbiol., 59(5), 1549-1554(1993). |
18 |
Hassairi, I., Amar, R. B., Nonus, M. and Gupta, B. B., "Production and Separation of |
19 | Ghadi, S. C., Muraleedharan, U. D. and Jawaid, S., "Screening for Agarolytic Bacteria and Development of a Novel Method for in Situ Detection of Agarase," J. Mar. Biotechnol. 5, 194-200 (1997). |
20 |
Toussaint, G., Ding, L. H., Jaffrina, M. Y., Hassairib, I. and Nonus, M., "Recovery of |
21 | Fu, W., Han, B., Duan, D., Liu, W. and Wang, C., "Purification and Characterization of Agarase from a Marine Bacterium Vibrio sp. F-6," J. Ind. Microbiol. Biotechnol. 35, 915-922(2008). DOI ScienceOn |
22 |
Fu, X. T., Lin, H. and Kim, S. M., "Purification and Characterization of a Novel |
23 | Levison, P. R., "Large-scale Ion-exchange Column Chromatography of Proteins Comparison of Different Formats, " J. Chromatogr. B, 790, 17-33(2003). DOI ScienceOn |
24 | Yamamoto, S. and Ishihara, T., "Ion-exchange Chromatography of Proteins Near the Isoelectric Points," Chromatogr. A, 852, 31- 36(1999). DOI ScienceOn |
25 | Johansson, H. J., Jagersten, C. and Shiloach, J., "Large Scale Recovery and Purification of Periplasmic Recombinant Protein from E. coli Using Expanded Bed Adsorption Chromatography Followed by New Ion Exchange Media," J. Biotechnol., 48, 9-14 (1996). DOI ScienceOn |
26 | Kupke, T., Stevanovic, S., Sahl, H.-G. and Gotz, F., "Purification and Characterization of EpiD, a Flavoprotein Involved in the Biosynthesis of the Lantibiotic Epidermin," J. Bacteriol., 174, 5354- 5361(1992). DOI |
![]() |