KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Determination of Lipid A Profile of Gram-Negative Bacteria from Arctic Soils Using Mass Spectrometric Approaches

질량분석 시스템을 이용한 극지 토양 유래 신규 미생물의 지질 A 화학적 구조 분석

  • Hwang, Cheol-hwan (Department of Chemical Engineering, Soongsil University) ;
  • Park, Han-Gyu (Department of Chemical Engineering, Soongsil University) ;
  • Kim, Yun-Gon (Department of Chemical Engineering, Soongsil University)
  • 황철환 (숭실대학교 화학공학과) ;
  • 박한규 (숭실대학교 화학공학과) ;
  • 김윤곤 (숭실대학교 화학공학과)
  • Received : 2016.10.18
  • Accepted : 2016.11.09
  • Published : 2016.12.31
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Abstract

For decades, the microorganisms in arctic soils have been newly discovered according to the climate change and global warming. In this study, the chemical structure of a lipid A molecule from Pseudomonas sp. strain PAMC 28615 which was newly discovered from arctic soils was characterized by mass spectrometric approaches such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI multi-stage tandem mass spectrometry (MS). First, lipopolysaccharide (LPS) from Pseudomonas sp. strain PAMC 28615 was extracted and subsequently hydrolyzed to obtain the lipid A. The parent ion peak at m/z 1632 was determined by MALDI-TOF MS, which also can validate our lipid A purification method. For detailed structural determination, we performed the multiple-stage tandem mass analysis ($MS^4$) of the parent ion, and subsequently the abundant fragment ions in each MS stage are tested. The fragment ions in each MS stage were produced from the loss of phosphate groups and fatty acyl groups, which could be used to confirm the composition or the position of the lipid A components. Consequently, the mass spectrometry-based lipid A profiling method could provide the detail chemical structure of lipid A from the Pseudomonas sp. strain PAMC 28615 as an arctic bacterium from the frozen arctic soil.

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References

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