• Proceedings of the Korean Sanitation Conference
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• 2004.11a
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• pp.45-53
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• 2004

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1284-1289
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• 2007

The Biological Activated Carbon (BAC) process in the water treatments represents a kind of biofiltration process which capabilities of bacteria to remove organic matters are maximized. It enables to eliminate organic matters and effectively reduce microbial regrowth potentials. As attached bacteria employ natural organic matter as a substrate, they are significantly dependent on indigenous microorganisms. In this study, characteristics of bacterial community by culturable and unculturable Methods have been conducted in a pilot plant using SAC in water treatment process at the downstream of the Nakdong River. Based on the results, HPC and bacterial- production for coal-based activated carbon material were $1.20{\sim}56.2{\times}l0^7$ cfu/g and $1.2{\sim}3.7\;mgC/m^{3}h$, respectively, in the SAC process. The highest level of attached bacteria biomass and organic carbon removal efficiency was found in the coal-based activated carbon. The genera Pseudomonas, Flavobacterium, Alcaligenes, Acilzetobacter, and Spingomonas were identified for each activated carbon material. Pseudomonas vesicularis was the dominant species in the coconut- and coal-based materials, where as Pseudomonas cepacia was the dominant species in the wood-based material. The Scanning Electron Microscope (SEM) observation of the activated carbon surface also found the widespread distribution of rod form and coccus. The community of attached bacteria was investigated by performing Fluorescent in situ hybridization (FISH) analysis. a group was dominant in coal, wood and coccunt-based materials, ${\alpha},\;{\beta}\;and\;{\gamma}$ group ranged from 27.0 ${\sim}$ 43.0%, 7.1 ${\sim}$ 22.0%, 11.3 ${\sim}$ 28.6%, respectively. These results suggest that a group bacterial community appears to be regulated removal efficiency of organic material in water treatment process.

• Journal of Life Science
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• v.27 no.9
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• pp.1086-1095
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• 2017

There is growing interest in groundwater resources to overcome the loss of surface water resources due to climate change. An understanding of the microbial community of aquifers is essential for monitoring and evaluating groundwater contamination, as well as groundwater management. Most microorganisms that inhabit aquifer ecosystems are attached to sediment particles rather than planktonic, as is the case in groundwater. Since sampling aquifer sediment is not easy, groundwater, which contains planktonic microorganisms, is generally sampled in microbial community research. Although many studies have investigated microbial communities in contaminated aquifers, there are only a few reports of microbial communities in uncontaminated or pristine aquifers, resulting in limited information on aquifer microbial diversity. Such information is needed for groundwater quality improvement. This paper describes the ecology and community structure of groundwater bacteria in uncontaminated aquifers. The diversity and structures of microbial communities in these aquifers were affected by the concentration or distribution of substrates (e.g., minerals, organic matter, etc), in addition to groundwater characteristics and human activities. Most of the microbial communities in these uncontaminated aquifers were dominated by Proteobacteria. Studies of microbial communities in uncontaminated aquifers are important to better understand the biogeochemical processes associated with groundwater quality improvement. In addition, information on the microbial communities of aquifers can be used as a basis to monitor changes in community structure due to contamination.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.1
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• pp.61-67
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• 2005

The objective of this study was to investigate community structure of attached algae and microbes in sedimentation basin of water and wastewater treatment plants by using respiratory quinone profile. There was an evident difference, in microbial community structure and attached algae species, between inclination plate settler and drainage canal in the sedimentation basin. The algae was composed of species in following order; Chlorophyceae>Bascillariophyceae>Cyanophyceae. The dominant quinone types of attached microorganisms in the wastewater treatment plant were plastoquinone (PQ)-9, vitamin(V)K-1 followed by UQ-8, but those for water treatment plant were VK-1, PQ-9 followed by UQ-8. These results suggests that nutrients, seasons and material of sedimentation basin have notable influence on composition of attached algae and microbial community structure in water and wastewater treatment plants.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.2
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• pp.113-120
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• 2010

Air-cathode microbial fuel cell consisted of 4 unit cells were operated under batch condition and electricity generation and microbial community structure variation were investigated, depending on separator types and cathode characteristics: A) PEM(Proton Exchange Membrane)-30% Wet proofing Carbon Cloth(WC), B) AEM(Anion Exchange Membrane-WC, C) CEM(Cation Exchange Membrane)-WC, D) PEM-No Wet proofing Carbon Cloth(NC). Maximum power densities of PEM-WC, AEM-WC and CEM-WC were 510.9, 522.1 and 504.8 $mW/m^2$, respectively. But PEM-NC showed relatively lower maximum power density of 218.3 $mW/m^2$. And PEM-WC, AEM-WC and CEM-WC showed similar internal resistances(20.0-28.2 ${\Omega}$). PCRDGGE, PCA and diversity indices showed that uncultured bacteria which reported in previous MFC studies were detected in suspended growth bacteria and attached growth bacteria would be affected not by separator type but by cathode characteristic. Thus, cathode characteristic can be one of the critical factors for power generation in air-cathode MFC using PEM, AEM, and CEM as separator.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.287-292
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• 2002

The composition of bacterial communities was detected in surface water of Cheonho Reservoir dominated by cyanobacteria, using fluorescent in situ hybridization (FISH) method. Total bacterial numbers were very high ranging from 0.6～$1.3{\times}10^7 \cells{\cdot}ml^-1$, whereas the ratio of Eubacteria to total bacteria was 29.8~45.8%, which was lower than that in other freshwater ecosystems. On average only 2.1% of DAPI-stained bacteria were detected by FISH with probes for $\alpha$, $\beta$, and $\gamma$-groups, respectively. Unknown eubacteria which was not bound to any probes except EUB 338, was relatively high. On the other hand, the Cytophaga-Flavobacterium group increased following the change of dominant species from Anabaena sp. to Microcystis sp. This result showed that bacterial communities could be affected by phytoplanktons, especially cyanobacteria.

• Korean Journal of Microbiology
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• v.31 no.3
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• pp.255-260
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• 1993

The Enterobacter cloacae biofilms developed on slide glasses and galvanized-iron coupons were applied to test the attached bacterial resistance to chlorination. The chlorine resistances of biofilm bacteria grown on the slide glasses and galvanized-iron coupons were 14 and 480 times that of the suspended bacteria, respectively. The chlorine resistance of particleattached bacterial populations was 48 times that of suspended bacterial populations. The biofilm bacterial densities developed on the slide glasses and galvanized-iron coupons which were immersed in the flowing tap water for 75 days were $4.75 {\times} 10^{4}$ and $1.12 {\times} 10^5 cfu/cm^{2}$ It is concluded that main mechanisms of enteric or HPC bacterial resistance to chlorination in tap waters are bacterial attachment or . adsorption to particles or bacterial aggregations and formation of biofilms on the inner wall of distribution systems by escaped bacteria from chlorination in water treatment processes, which results in bacterial regrowth in water distribution systems.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.11
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• pp.1161-1169
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• 2008

The removals of TCE and PCE vapor with or without a supply of toluene as a primary substrate were compared in a biofiltration process, and the variations of microbial communities associated with the removal were also investigated. As a result of investigations on the removals of TCE/PCE in a biofilter B within which TCE/PCE-acclimated sludge was attached on the surface of media without a supply of primary substrate, and those in another biofilter A where toluene-acclimated sludge was attached with a supply of toluene as a primary substrate, followings were found: (i) parts of microbes responsible to the decomposition of toluene vapor participate in the removal of chlorinated VOCs such as TCE and PCE, and (ii) effective biological removals of TCE and PCE vapor do not necessarily need cometabolism. Sequencing of 16S rDNA obtained from the band profile of DGGE (Denaturating Gradient Gel Electrophoresis), it was confirmed that: (i) uncultured alpha proteobacterium, uncultured Desulfitobacterium, uncultured Rhodobacteraceae bacterium, Cupriavidus necator, and Pseudomonas putida were found to be toluene-decomposing microbes, (ii) alpha proteobacterium HTCC396 is a TCE-removing microbe, (iii) Desulfitobacterium sp. is a PCE-decomposing microbe, and (iv) particularly, uncultured Desulfitobacterium sp. is probably a microbe decomposable not only toluene but also various chlorinated VOC vapor including TCE and PCE.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.203.2-203.2
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• 2016

미생물이 스스로 생성한 고분자 물질에 싸이며 군집체를 형성한 바이오필름은 고체 표면에 부착되며 우리 생활 속에 다양한 형태로 발견할 수 있다. 바이오필름은 미생물에 적합하지 않은 외부 환경으로부터 미생물 스스로 보호하는 기능을 하며, 형성된 바이오필름은 오랜 기간 동안 생존하여 살균제나 항생제로부터 저항성을 가져 살균과정에서 제거되지 않고 2차오염을 야기할 수 있어 식품 가공 기계 및 수도관, 의료기기 등에 형성되었을 경우 식품 오염, 상처 감염 등의 원인이 된다. 이 때문에 위생과 바이오필름의 상관관계를 인지하고 이를 제어하기 위한 연구가 여러 방법으로 진행되고 있다. 대표적인 방법으로는 천연 향균제 개발, 쿼럼 센싱(Quoroum sensing)과 같이 미생물의 신호전달 체계를 차단하는 물질 개발 및 플라즈마 처리 등이 있다. 본 연구에서는 격자형식의 유전장벽방전(DBD) 형식의 플라즈마 소스를 개발하여 바이오필름의 효과적인 제어 가능성을 확인하고, 제어 방식의 관계를 파악하였다. 플라즈마 처리 대상의 화학적 분석을 위하여 유기물질 등을 사용해 플라즈마 처리수 내 화학물질 분석 시스템을 구축하여 이를 기반으로 플라즈마로 생성된 HNO2, NO2-, H2O2 등의 화학종이 가지는 바이오필름 제어 관계를 살펴보았으며, 화학적 방법인 제어효과와 비교하여 플라즈마의 바이오필름 제어 특성에 대해 살펴보았다. 본 발표에서 플라즈마의 바이오필름 제어효과에 대한 분석에 대해 더 자세한 결과가 발표될 예정이다.

• Korean Journal of Microbiology
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• v.52 no.2
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• pp.125-139
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• 2016

Biofilms are considered a complexly structured community of microorganisms derived from their attached growth to abiotic and biotic surfaces. In human life, they mediate serious infections and cause many problems in civil and industrial facilities. While it is of huge interest for scientists to understand biofilms, it has been very hard to directly analyze the various biofilms in nature. A variety of biofilm models have been suggested for laboratory-scale biofilm formation and many methods based on these models are widely used for the biofilm researches. These biofilm models mimic characteristics of environmental biofilms with different advantages and disadvantages. In this review, we will introduce these currently used biofilm model systems and explain their relative merits.