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A Study on the TCE/PCE Removal Using Biofiltration and the Microbial Communities Variation Using DGGE Method  

Kim, Eung-In (Department of Environmental Engineering, Pusan National University)
Park, Ok-Hyun (Department of Environmental Engineering, Pusan National University)
Jung, In-Gyung (Pohang Eco Industrial Park, Research institute of industrial Science & Technology)
Publication Information
Journal of Korean Society of Environmental Engineers / v.30, no.11, 2008 , pp. 1161-1169 More about this Journal
Abstract
The removals of TCE and PCE vapor with or without a supply of toluene as a primary substrate were compared in a biofiltration process, and the variations of microbial communities associated with the removal were also investigated. As a result of investigations on the removals of TCE/PCE in a biofilter B within which TCE/PCE-acclimated sludge was attached on the surface of media without a supply of primary substrate, and those in another biofilter A where toluene-acclimated sludge was attached with a supply of toluene as a primary substrate, followings were found: (i) parts of microbes responsible to the decomposition of toluene vapor participate in the removal of chlorinated VOCs such as TCE and PCE, and (ii) effective biological removals of TCE and PCE vapor do not necessarily need cometabolism. Sequencing of 16S rDNA obtained from the band profile of DGGE (Denaturating Gradient Gel Electrophoresis), it was confirmed that: (i) uncultured alpha proteobacterium, uncultured Desulfitobacterium, uncultured Rhodobacteraceae bacterium, Cupriavidus necator, and Pseudomonas putida were found to be toluene-decomposing microbes, (ii) alpha proteobacterium HTCC396 is a TCE-removing microbe, (iii) Desulfitobacterium sp. is a PCE-decomposing microbe, and (iv) particularly, uncultured Desulfitobacterium sp. is probably a microbe decomposable not only toluene but also various chlorinated VOC vapor including TCE and PCE.
Keywords
PCE; TCE; Biofiltration; PCR; DGGE;
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Times Cited By KSCI : 4  (Citation Analysis)
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