The Bacterial Community Structure in Cheonho Reservoir Dominated by Cyanobacteria

봄철 Cyanobacteria 가 우점한 천호지에서 세균군집구조의 변화

  • 홍선희 (단국대학교 생명과학부 미생물학과) ;
  • 전선옥 (강원대학교 자연과학대학 환경학과) ;
  • 안태석 (강원대학교 자연과학대학 환경학과) ;
  • 안태영 (단국대학교 생명과학부 미생물학과)
  • Published : 2002.12.01

Abstract

The composition of bacterial communities was detected in surface water of Cheonho Reservoir dominated by cyanobacteria, using fluorescent in situ hybridization (FISH) method. Total bacterial numbers were very high ranging from 0.6~$1.3{\times}10^7 \cells{\cdot}ml^-1$, whereas the ratio of Eubacteria to total bacteria was 29.8~45.8%, which was lower than that in other freshwater ecosystems. On average only 2.1% of DAPI-stained bacteria were detected by FISH with probes for $\alpha$, $\beta$, and $\gamma$-groups, respectively. Unknown eubacteria which was not bound to any probes except EUB 338, was relatively high. On the other hand, the Cytophaga-Flavobacterium group increased following the change of dominant species from Anabaena sp. to Microcystis sp. This result showed that bacterial communities could be affected by phytoplanktons, especially cyanobacteria.

봄철 cyanobarteria가 우점하는 천호지에서 fluorescent in situ hybridization (FISH) 방법을 이용하여 세균군집구조를 조사하였다. 조사기간동안 총세균수는 0.6~$1.3{\times}10^7 \cells{\cdot}ml^-1$ 의 범위로 매우 높게 나타났다. 또, 총세균수에 대한 eubacterial group이 차지하는 비율의 경우 29.8~45.8%로 작은 분포를 차지하였다. 세균군집구조를 분석한 결과, $\alpha$-group은 1.4~l2.5%, $\beta$-group은 0.9~4.9%, $\gamma$-group은 0.6~8.3%, $\circledcirc$Cytophaga-Flavobacteruim group은 1.0~8.3%를 나타내어 본 연구에서 조사된 각 group이 차지하는 비율은 낮고 상대적으로 unknown eubacteria의 비율이 높았다. 이러한 군집구조는 일반적으로 다른 담수호에서 나타나는 세균의 군집구조와는 다른 군집구조 이며 특히, aggregates에 부착한 세균 군집구조와 비슷한 양상이었다. 또, cyanobacteria의 조성 이 Anabaena속에서 Microcystis속으로 천이가 일어난 시기에 Cytophaga-Flavobacteruim group이 크게 증가하는 것으로 나타나 세균군집의 크기는 식물플랑크톤의 영향을 받는 것으로 확인되었다.

Keywords

References

  1. 한국육수학회지 v.28 Cyanobacteria의 증식에 따른 대청호 생태계내의 생물군집 변화 김명운;김민호;조장천;김상종
  2. 한국미생물학회지 v.30 소양호에서 동물플랑크톤의 섭식 작용에 관한 연구 심두섭;안태석
  3. 한국육수학회지 v.31 하천수중에서 식물플랑크톤의 증식에 대한 희석율과 온도의 영향 전만식;Yasunori Watanabe;김범철
  4. 한국담수조류도감 정준
  5. 한국육수학회지 v.31 동복호에서 장해성 조류의 계절별 동태 및 환경요인간의 상관성 조영관;정진;이진종
  6. 한국미생물학회지 v.31 소양호에서 세균수와 세균체적의 계절적 수심별 변화 안태석;최승익;변명섭;박호완
  7. 강원대학교 대학원 이학박사 학위논문 담수생태계에서 세균군집구조의 분석 홍선희
  8. 한국미생물학회지 v.36 팔당호에서 Aggregates에 부착한 세균군집구조의 변화 홍선희;오덕화;전선옥;안태석
  9. Appl. Environ. Microbiol. v.62 Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization Alfreidner, A.;J. Pernthhaler;R. Amann;B. Sattler;F.O. Glockner;A. Wille;R. Psenner
  10. Prog. Oceanogr. v.20 Characteristics, dynamics, and significance of marine snow Alldredge, A.L.;M.W. Silver
  11. Microbiol. Rev. v.59 Phylogenetic and in situ detection of individual microbial cells without cultivation Amann, R.;W. Ludwig;K.H. Schleifer
  12. Appl. Environ. Microbiol. v.66 Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescent in situ hybridization Cottrell, M.T.;D.L. Kirchman
  13. Appl. Environ. Microbiol. v.65 Bacterioplankton compositions of lakes and oceans: A first comparison based on fluorescence in situ hybridization Glockner, F.O.;B.M. Fuchs;R. Amann
  14. Appl. Environ. Microbiol. v.58 Dual staining of natural bacterioplankton with 4,6-diamidino-2-phenylindole and fluorescent oligonucleotide porbes targeting kingdom level 16S rRNA sequences Hicks, R.;R.I. Amann;D.A. Stahl
  15. Appl. Environ. Microbiol. v.65 Seasonal dynamics of bacterioplankton community structure in a eutriphic lake as determined by 5S rRNA anaysis Hofle, M.G.;Heike;Haas;K. Dominik
  16. J. Bacteriol. v.180 Impact of culture-independent studies on the emerging phylogenetic view of bacterial community Hugenholtz, P.;B.M. Gloebel;N.R. Page
  17. Appl. Environ. Microbiol. v.64 Seasonal community and population dynamics of pelagic bacteria and archaea in a high mountain lake Jakob, P.:F.O. Glockner;S. Unterholzner;A. Alfreidner;R. Psenner;R. Amann
  18. Arch. Hydrobiol. v.98 The gram-stain characteristics of the bacterial community as function of the dynamics of organic debris in a mesotrophic irrigation pond Kang, H.;H. Seki
  19. FEMS Microbiol. Ecol. v.39 The ecology of Cytophaga-Flavobacteria in aquatic ecosystem Kirchman, D.
  20. Microb. Ecol. v.42 Investigating influential factors on bacterioplankton community composition: Results from a field study of five mesotrophic lakes Lindstrom, E.S.
  21. Deep-Sea ResearchⅡ v.42 Rapid formation and sedimentation of large aggregates is predictable from coagulation rates(half-lives) of transparent exopolymer particles(TEP) Logan, B.E.;Passow, U.;Alldredge, A.L.;Grossart, H.P.;M. Simon
  22. Appl. Environ. Microbiol. v.64 Seasonal and spatial variability of bacterial and archaeal assemblages in the coastal waters near Anvers Island, Antarctica Murray, A.E.;C.M. Presston;R. Massana;L.T. Taylor;A. Blakis, K.W.U.;E.F. DeLong
  23. The Ecology of Cyano-Bacteria (1st ed.) Freshwater blooms Oliver, L.R.;G.G. Ganf;Whitton B.A.(eds.);Potts M.(eds.)
  24. A Biology of the Algae (2nd ed.) Prokaryotic Algae (Cyanophyta, Prochlorophyta) Philip Sze.;Philip Sze(ed.)
  25. Appl. Environ. Microbiol. v.65 In situ analysis of phototrophic sulfur bacteria in the chemocline meromictic lake Cadagno(Swizerland) Tonolla, M.;A. Demarta;R. Peduzzi;D. Hahn
  26. Appl. Environ. Microbiol. v.65 Changes in bacterial and eukaryotic community structure after mass lysis of filamentous cyanobacteria associated with viruses Van Hannen, E.J.;G. Zwart;M.P.V. Agterveld;H.J. Gons;J. Ebert;G.J. Laanbroek
  27. 日本淡水藻圖鑑 內田老鶴圃 廣瀨弘辛