• 제목/요약/키워드: 방치시간

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Preanalytical Stability of Adrenocorticotropic Hormone Depends on Time to Centrifugation (전혈에서 시간과 온도에 따른 Adrenocorticotropic Hormone의 안정성 평가)

  • Shin, Sun-Young;Lee, Hyun-Joo;Min, Gyung-Sun;Lee, Ho-Young
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.1
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    • pp.116-119
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    • 2009
  • Background: Preanalytical factors can affect reliability of hormone assay results. Adrenocorticotropic hormone (ACTH) in blood is considered highly unstable because of proteolytic degradation, so storage of blood samples on ice until analysis is recommended. In clinical practice, however, this procedure may present logistical problems because most samples for ACTH measurement must be shipped from the place of sample collection to the laboratory. Therefore, we studied the impact of time and temperature before plasma separation and analysis on the results of ACTH assays. Methods: A total number of 22 patients were enrolled in this study. We obtained 2 blood samples. ACTH concentrations were 35~126 pg/mL. ACTH concentrations were measured by immunoradiometric assay (IRMA) using commercial kits (CIS Biointernational, Gif-sur-Yvette, France). Results: ACTH levels showed a significant difference between the samples of $22^{\circ}C$ EDTA and $4^{\circ}C$ EDTA. Measured ACTH concentrations significantly decreased with time before freezing at $-20^{\circ}C$. ACTH levels showed no significant difference between the groups of after storage for 24 hr without centrifugation at $22^{\circ}C$ and $4^{\circ}C$. Conclusion: We recommend that blood samples be obtained on pre-chilled EDTA collection tubes. The shortest possible time between sample collection and processing is always the best laboratory practice.

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Studies on the Mulberry Graftages IV. Cold Hardiness in December (뽕나무 접목묘에 관한 연구 IV. 12월 중의 내동성)

  • Lee, Won-Ju;Lee, Ju-Won;Park, Gwang-Jun
    • Journal of Sericultural and Entomological Science
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    • v.31 no.2
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    • pp.65-68
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    • 1989
  • Cold hardiness of mulberry graftages was examined with two varieties, Kaeryangppong and Keomseolppong in early December. Exposure temperatures were three levels (-5, -10 and -15$^{\circ}C$) at the 4 exposure times (6, 12, 24 and 48 hrs). No damage showed at -5$^{\circ}C$ for 48 hrs. Survival rate decreased sharply from -10$^{\circ}C$, regardless exposure times. There was no survivals from -10$^{\circ}C$ for 12 hrs in Kaeryangppmong and from -15$^{\circ}C$ for 6 hrs in Keomseolppong. This suggested Keomseolppong was stronger in cold than kaeryangppong. Regrowth decreased drastically from -10$^{\circ}C$.

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Changes in Antimicrobial Activity of Hydrolyzate from Mustard Seed (Brassica juncea) (겨자 가수분해물의 항균성 변화)

  • 서권일;박석규;박정로;김홍출;최진상;심기환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.1
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    • pp.129-134
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    • 1996
  • 겨자의 가수분해 시간에 따른 향균력을 비교하고, 각종 미생물에 대한 겨자 물 추출물의 최소 저해 농도 및 최소 저해 투여량을 측정한 결과 다음과 같았다. 겨자의 자가분해 시간에 따른 물 추출물의 향균성은 처음에 아주 약하게 나타났던 것이 12시간 방치 후 크게 증가하였다. 최대 항균활성은 24시간 방치 후 나타났으며 이후 거의 변하지 않았다. 겨자를 첨가한 물의 산도는 시간이 지남에 따라 증가하였고, 겨자의 농도가 클수록 그 정도가 크게 나타났다. $37^{\circ}C에서$ 24시간 자가분해한 겨자 물 추출물의 MID는 Bacillus subtilils가 $70\mu1/disc로$ 다른 실험 균주에 비하여 낮았으며, 젖산균이 $95\mu1/disc로서$ 높게 나타났다. MIC는 Bacillus subtilils, Staphylococcus aureus, Salmonella typhimurium 및 Vibrio parahaemolyticus 균이 $70\mu1/ml로$ 다른 실험 균주에 비하여 낮게 나타났으며, 젖산균 곰팡이에서 $90\mu1/ml로$ 높게 나타났다. 자가분해 시간에 따른 겨자물 추출물의 중류성분은 자가분해 시간이 지남에 다라 점차 증가하는 경향이었고, isothiocyanate 류의 함량은 24시간 때에 최대치를 나타내었다.

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Bonding Properties and Resin Exudation Characteristics of Pitch Pine (리기다소나무재의 수지 삼출성과 접착 특성)

  • Roh, JeongKwan;Kim, Yun Geun
    • Journal of the Korean Wood Science and Technology
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    • v.46 no.3
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    • pp.213-220
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    • 2018
  • In order to use Pitch pine (Pinus rigida Miller) as the material of the structural glued laminated timber, the effect of the amount of resin exudation due to storage time after the planning and the knot of the lamina were evaluated on the bonding properties of the glued board with resorcinol resin. For Pitch pine that was dried at high temperature ($120{\sim}95^{\circ}C$) and low temperature ($65{\sim}50^{\circ}C$), the flat sawn(tangential section) showed higher amount of resin exudation than the quarter sawn(radial section). And the low temperature drying wood showed higher resin exudation than the high temperature drying wood. The low and high temperature drying wood showed the highest amount of resin exudation on the 3rd day and 7th day, respectively and they were gradually decreased. However, there were no significant differences from 15 to 90 days. Adhesion performances were low until 2~3 days with high exudation of resin, but there were no significant differences after 15 days. Both high temperature and low temperature drying woods satisfied the Korean standard regardless of the storage time. The adhesive strengths of the laminating parts including knots were higher than those of KS criteria, but the wood failures were not satisfied the KS standard. Adhesive performances according to the laminating combinations (quarter sawn + quarter sawn, flat sawn + flat sawn, quarter sawn + flat sawn) were better than those of KS criteria in all laminating combinations in both high temperature and low temperature drying woods.

Measurement of $^{222}Rn$ in ground water with low-level Liquid Scintillation Counter and pulse shape analysis (저준위 액체섬광계수기와 파형분석법을 이용한 지하수중의 $^{222}Rn$ 측정)

  • Jo Su-Yeong;Yun Yun-Yeol;Lee Gil-Yong;Kim Yong-Je
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2006.04a
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    • pp.319-323
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    • 2006
  • 파형분석(PSA) 기능과 백그라운드 낮고 계측효율이 높은 장점을 가지고 있는 저준위 액체섬광계수기를 이용하여 지하수중의 $^{222}Rn$ 측정을 위한 최적 분석조건을 확립하였다. 라돈분석을 위해 섬광용액 HiSafe 3 12 ml를 사용하여 물시료 8 ml 내 $^{222}Rn$ 을 측정하였다. 라돈은 딸핵종과의 방사평형을 위해 3시간동안 방치한 후 계측하였다. 최적 파형분석 (PSA) 준위는 100 이었다. $^{222}Rn$의 계측효율은 $^{226}Ra$ 표준시료를 동일 조건으로 제조한 후 약 20일 이상 방치한 다음 측정하여 결정하였으며 측정효율은 약 $91.6{\pm}3.6%$ 이었다. 동일 시료의 라돈 추출실험 재현성은 2 % 이내이었다. 계측시간 10시간을 기준으로 바탕값은 0.035 cpm 이었고 300분 계측시 검출하한값은 0.11 Bq/L 이었다.

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Studies on the Electrochemical Properties for Rancidity of Linoleic Acid (리놀산의 산패에 대한 전기화학적 특성 연구)

  • 김우성;이송주
    • The Korean Journal of Food And Nutrition
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    • v.13 no.4
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    • pp.360-364
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    • 2000
  • We studied the degree of rancidity of linoleic acid for the electrochemical redox reaction in time course and the kinetic parameters. The current of the linoleic acid was increased and the potential was shifted to the positive potential when scan rates were faster. The redox reaction of the linoleic acid was proceeding to totally irreversible and diffusion controlled reaction. From these results, diffusion coefficient(D$\_$o/) of linoleic acid was observed to 2.61$\times$10$\^$-6/ ㎠/s in the 0.1 M TEAP/DMF electrolyte solution. Also, exchange rate constant(K$\^$o/) was observed to 9.79$\times$10$\^$-11/ cm/s. The leaving time in air condition was found to affect the rancidity. We predicted that the product was carbonyl compounds.

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Gelation Behavior of Acrylonitrile Copolymer/Dimethylformamide Solution and Mechanical Properties of Films Obtained from It′s Solution (아크릴로니트릴공중합체-디메틸포름아마이드 용액의 겔화 거동 및 겔필름의 물성)

  • 오영세;한삼숙;송기원
    • Polymer(Korea)
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    • v.24 no.6
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    • pp.787-793
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    • 2000
  • The acrylonitrile copolymer/dimethylformamide (DMF) solutions were prepared to investigate the gelation behavior and critical gel concentration (c*). Gelation is rapidly progressed with the increase of molecular weight of copolymer, but significantly delayed with supercooling temperature and comonomer contents. The c* behavior showed contrary trend against gelation behavior. In dynamic viscoelastic test, two glass-transition region were observed in film obtained from gelled solution whereas one glass-transition in film obtained from true solution. This result supports the idea that an ordered junction zone is formed by the dipole-dipole interaction of intermolecularly neighboring stereo-regular parts of atactic acrylonitrile copolymer chains due to a nucleation process in the solution.

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Release of Hyaluronidase and Arylsulfatase from Rabbit Sperm Acrosomes into Seminal Plasma (하이얄유로니데이스 및 아릴쎌파테이스의 토끼 정자 아크로좀으로부터 정액으로의 배출)

  • Yang, Chul-Hak
    • Clinical and Experimental Reproductive Medicine
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    • v.8 no.2
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    • pp.25-28
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    • 1981
  • 정자의 아크로좀에 존재한다고 알려진 효소들중 실온에 방치할 경우 또는 완충용액으로 정자를 세척할 경우 쉽게 아크로좀 막으로부터 흘러나옴을 관찰하였다. 신선하게 사용된 토끼의 정액을 $0^{\circ}C$에서 트리스 완충용액에 방치할 경우 하이얄유로니데이스는 39%정도가 유출되어 나오고, 또 $37^{\circ}C$에서 1시간 더 둘 경우 나머지 30%, 그리고 두시간째에 17%가 방출되며, 계면활성제로 아크롬을 처리할 경우 단 4%의 효소가 추출되었다. 다른 두 탄수화물 분해요소인 베타 엔 애시틸글루코사미니데이스 및 베타 그룩유로니데이스도 하이얄유로니데이스처럼 쉽게 분리되어 나오고 계면활성제로 추출될 수 있는 양은 대단히 적었다. 이와같이 효소가 아크로좀에서 방출되는 정도에 따라 아크로좀막에 흡착된 정도를 알 수 있으며 동시에 수정과정에서 역활을 이해하는데 도움이 될 것이다.

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질병 핫이슈 - 붉어도 너~무 붉어~ 방치하면 악화되는 안면홍조증

  • Kim, Jeong-Un
    • 건강소식
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    • v.36 no.12
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    • pp.28-29
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    • 2012
  • 날씨가 추워지면서 혈관 수축이 제대로 기능하지 못해 심각해지는 질환이 바로 안면홍조증 이다. 단기간에 치료하기 어렵고, 많은 사람들이 '이러다 괜찮아지겠지'라고 생각하지만 내버려두면 시간이 흐를수록 악화되기 십상이다. 꾸준한 치료와 관리가 필요하다.

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