• Title/Summary/Keyword: 박상동

Search Result 153, Processing Time 0.029 seconds

Analysis on the Characteristics of the Landslide in Maeri (I) - With a Special Reference on Geo-Topographical Characteristics - (매리 땅밀림형 산사태(山沙汰)의 발생특성(發生特性)에 관한 분석(分析) (I) - 지형(地形) 및 지질특성(地質特性)을 중심(中心)으로 -)

  • Park, Jae-Hyeon;Choi, Kyung;Bae, Jong Soon;Ma, Ho-Seop;Lee, Jong-Hak
    • Journal of Korean Society of Forest Science
    • /
    • v.94 no.3 s.160
    • /
    • pp.129-134
    • /
    • 2005
  • Ths study was carried out to understand the geo-topographical characteristics of the landslide area (1.5 ha) in Maeri, Sangdong-myeon, Gimhaesi, Gyeongsangnam-do. The bedrock of the landslide area was diollites and the area in and around the upper slope with the scattered talus was composed of Masanam originated from Igneous rocks. The landslide occurred in 19th of April, 2004 and the amount of rainfall for 3 days before the landslide was 74mm. The landslide by land creeping in this area was mainly attributed to geo-topographical characteristics such as well developed colluvial and/or weathered soils, and land cutting in lower slope, although the landslide could be affected by the rainfall. The type of the landslide can be classified into the land creeping by concave colluvial soils.

Isolation of an Acid-Labile Gene from the Seaweed Porphyra yezoensis Tissue (해조류 김 Porphyra yezoensis 엽체로부터 산에 민감한 유전자의 분리)

  • Jin, Hyung-Joo;Park, Sun-Mee;Kim, Long-Guo;Jin, Deuk-Hee;Kong, In-Soo;Hong, Yong-Ki
    • KSBB Journal
    • /
    • v.14 no.6
    • /
    • pp.702-706
    • /
    • 1999
  • The genetic responses of aquaculturable seaweed Prophyra yezoensis tissue by acid shock have been compared using differential display technique. The tissue has been challenged in seawater containing 0.05% hydrogen chloride(pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs, respectively. Total RNA was extracted by LiCl-guanidinium method. The cDNA was synthesized by reverse transcription with random hexamers and amplified by PCR with arbitrary primers. The genetic fragment disappeared by acid shock was selectively isolated from agarose gel and sequenced with a DNA auto sequencer. One of the acid-labile gene(605 bp) was identified as a dethiobiotin synthetase gene according to sequence alignment analysis by the NCBI BLAST search program.

  • PDF

Characterization of Cucumber mosaic virus Isolated from Trifolium repens in Korea (국내 토끼풀에서 분리한 Cucumber mosaic virus의 특성)

  • Park, Tae Seon;Choi, Gug Seoun;Hong, Jin Sung
    • Research in Plant Disease
    • /
    • v.22 no.1
    • /
    • pp.55-58
    • /
    • 2016
  • A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showing mosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified through confirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysis of coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco species including Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. In Chenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMV-Tr1 compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5% homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP gene indicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is the first report of CMV in T. repens in Korea.

A Study on Detecting Moving Objects using Multiple Fisheye Cameras (다중 어안 카메라를 이용한 움직이는 물체 검출 연구)

  • Bae, Kwang-Hyuk;Suhr, Jae-Kyu;Park, Kang-Ryoung;Kim, Jai-Hie
    • Journal of the Institute of Electronics Engineers of Korea SP
    • /
    • v.45 no.4
    • /
    • pp.32-40
    • /
    • 2008
  • Since vision-based surveillance system uses a conventional camera which has a narrow field of view, it is difficult to apply it into the environment whose the ceiling is low and the monitoring area is wide. To overcome this problem, the method of increasing the number of camera causes the increase of the cost and the difficulties of camera set-up For these problems, we propose a new surveillance system based on multiple fisheye cameras which have 180 degree field of view. The proposed method handles occlusions using the homography relation between the multiple fisheye cameras. In the experiment, four fisheye cameras were set up within the area of $17{\times}14m$ at height of 2.5 m and five people wandered and crossed with one another within this area. The detection rates of the proposed system was 83.0% while that of a single camera was 46.1%.

Real-Time Eye Detection and Tracking Under Various Light Conditions (다양한 조명하에서 실시간 눈 검출 및 추적)

  • 박호식;박동희;남기환;한준희;나상동;배철수
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2003.10a
    • /
    • pp.227-232
    • /
    • 2003
  • Non-intrusive methods based on active remote IR illumination for eye tracking is important for many applications of vision-based man-machine interaction. One problem that has plagued those methods is their sensitivity to lighting condition change. This tends to significantly limit their scope of application. In this paper, we present a new real-time eye detection and tracking methodology that works under variable and realistic lighting conditions. eased on combining the bright-pupil effect resulted from IR light and the conventional appearance-based object recognition technique, our method can robustly track eyes when the pupils are not very bright due to significant external illumination interferences. The appearance model is incorporated in both eyes detection and tracking via the use of support vector machine and the mean shift tracking. Additional improvement is achieved from modifying the image acquisition apparatus including the illuminator and the camera.

  • PDF

Molecular Cloning and Nucleotide Sequence of the G protein of a Korean Isolate of Infectious Hematopoietic Necrosis Virus (한국에서 분리된 IHNV-PRT의 G protein의 유전자 클로닝과 염기서열 분석)

  • 김영조;허강준;박정우;박정문
    • Korean Journal of Microbiology
    • /
    • v.35 no.3
    • /
    • pp.226-230
    • /
    • 1999
  • To characterize the Korean isolate of infeciious hematopoietic necrosis virus (IHNV-PRT), a partial DNA fragment G gene of the MNV-PRT was amplified by RT-PCR. cloned inlo pGEM-T easy vector and analyzed for nucleotlde sequences. The size of the PCR pmduct was about 442 bp. The nucleotlde sequence homologies ofthe G gene of IHNV-PRT were 95%, 94%, 94% 94%, 93%, 53%. respectively. with those of foreign isolates of IHNV, IHNV-RB-76. IHNV-LR-73, MNV-K, IHNV-WRAC, Im-SRCV, IHNV-Col-85. However, it showed 81% homology with that of other fish rhabdovirus, hisame rhabdovirus (HRV). Frou~ the rcsults of deduced amino acid sequence homology analysis. G protein of IHNV-PRT showed 96% hornologies with those of foreign isolates of IHNV but 89% homology with that of HRV These results indicaled that, even though G gene of IHIW-PRT showed low homology with that of HRY it was highly conserved among different strains of THNV.

  • PDF

Analysis on the Characteristics of the Landslide in Maeri (IV) - With a Special Reference on Stability Analysis of Structures - (매리 땅밀림형 산사태(山沙汰)의 발생특성(發生特性)에 관한 분석(分析) (IV) - 구조물(構造物)의 안정해석(安定解析)을 중심(中心)으로 -)

  • Park, Jae-Hyeon;Bae, Jong Soon;Choi, Kyung;Ma, Ho-Seop;Lee, Jong-Hak;Youn, Ho-Jung
    • Journal of Korean Society of Forest Science
    • /
    • v.95 no.1
    • /
    • pp.5-11
    • /
    • 2006
  • This study was carried out to analyse the landslide characteristics by structure stability analysis at the landsliding area occurred in Maeri, Sangdong-myeon, Gimhaesi, Gyeongsangnam-do. External stability of the retaining wall and the retaining stonewall was accessed as unstable condition except for the II-D profile. It is needed to consider a counter plan to stabilize the structure. Also, it is necessary to stabilize the unstable retaining wall reinforced with adding draining measure. It is useful to reduce the load from upper retaining stonewall by alleviating the slope degree from stonewall structures.

Cloning and Nucleotide Sequence Analysis of the rpoH Gene from Methylovorus sp. Strain SS1 DSM11726 (Methylovorus sp. Strain SS1 DSM11726으로부터 rpoH 유전자의 클로닝과 염기서열 분석)

  • Eom, Chi-Yong;Song, Seung-Eun;Park, Mi-Hwa;Kim, Young-Min
    • Microbiology and Biotechnology Letters
    • /
    • v.35 no.3
    • /
    • pp.177-183
    • /
    • 2007
  • Using complementation of RpoH deficient E. coli strain A7448, the rpoH gene encoding heat shock sigma factor 32 (${\sigma}^{32}$) from Methylovorus sp. strain SS1 DSM11726 was cloned and sequenced. Sequence analysis of a stretch of 1,796-bp revealed existence of an open reading frame encoding a polypeptide of 284 amino acid (32,006 dalton). Deduced amino acid sequence of the Methylovorus sp. strain SS1 RpoH showed that 59.6%, 39.1% and 51.4% identities with those of Nitrosomonas europaea (${\beta}$-proteobacteria), Agrobacterium tumefaciens ($\alpha$-proteobacteria) and E. coli (${\gamma}$-proteobacteria). The expression level of the functional ortholog of RpoH of Methylovorus sp. strain SS1 was increased transiently after heat induction, further indicating that it functions as a heat shock sigma factor.

Characteristics of Pichia anomala K15 Producing Killer Toxin Isolated from Traditional Nuruk (전통 누룩으로부터 분리된 Killer Toxin 생산 균주 Pichia anomala K15의 특성)

  • Jung, Hee-Kyoung;Park, Chi-Duck;Lee, Gee-Dong;Park, Seung-Chun;Park, Hwan-Hee;Hong, Joo-Heon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.36 no.8
    • /
    • pp.1077-1082
    • /
    • 2007
  • In this study, killer yeasts were isolated from traditional Nuruk to improve storage and suppress contaminant in food industry. Among killer yeasts, yeast K15 showed strong killer toxin activity and inhibited growth of Salmonella Typhimurium and Vibrio parahaemolyticus. Killer yeast K15 was identified with Pichia anomala by the Microlog TM 4.0 identification system and homology of the ITS sequence. Killer toxin generated from P. anomala K15 was inactivated by pronase E and suggested to be a protein. Therefore killer toxin of P. anomala K15 was thought to be safe in human such as bacteriocin. P. anomala K15 was sufficient for growth in 50% glucose and could be used to prevent contaminant in initial stages of alcohol beverage fermentation.

Screening of Lactic Acid Bacteria as a Starter Culture in Fermented Sausage (발효소시지 제조에 적합한 스타터 선발)

  • Yoo, Seon-A;Seo, Seung-Ho;Park, Seong-Eun;Son, Hong-Seok
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.43 no.8
    • /
    • pp.1289-1295
    • /
    • 2014
  • The aim of this study was to select the most suitable starter cultures for production of fermented sausages. A total of 27 strains isolated from Korean fermented foods and natural substances were characterized with respect to their physicochemical properties in a fluid (submerged) model system modified according to the special conditions of fermented sausages. Three of these strains were pre-selected for testing as potential cultures based on their ability to grow fast and initiate rapid acidification. The selected strains were identified by API and partial sequence analysis of 16S rRNA. The results exhibited sequence similarity to known sequences of Staphylococcus warneri, Staphylococcus epidermidis and Lactobacillus plantarum. Among them, relatively good growth properties and nitrite reduction activities were detected for S. epidermidis and L. plantarum and low pH values and high total acidities were observed in the model system fermented with these isolates compared with reference strains.