• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.312-325
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• 2015

In this review, we summarized the trends of genomics and transcriptomics research on peach, a model species of Rosaceae. Peach genome maps have been developed from various progeny groups with many next-generation sequencing (NGS) based single nucleotide polymorphism markers. Molecular markers of qualitative traits and quantitative trait loci (QTL) such as fruit characteristics, blooming date, and disease resistance have been analyzed. Among many characteristics, markers related to flesh softening and flesh adhesion are useful for marker assisted selection. Through comparative genomics, peach genome has been compared to the genome of Arabidopsis, Populus, Malus, and Fragaria species. Through transcriptomics and proteomics, fruit growth and development, and flavonoid synthesis, postharvest related transcriptomes and disease resistance related proteins have been reported. Recently, development of NGS based markers, construction of core collection of germplasm, and genotyping of various progenies have been preceded. In the near future, accurate QTL analysis and identification of useful genes are expected to establish a foundation for effective molecular breeding.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.46 no.3
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• pp.1-7
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• 2009

This paper suggests an automatic electrophoretic deposition (EPD) robot system using a color mono camera. The EPD robot system consists of a 2 DOF rectangular robot to practically carry out EPD tasks, and a color mono camera to automatically control the robot. The screws are used to actuate the robot with low speed of 10mm/s for EPD tasks. The color mono camera detects the color marks attached to the robot and beakers, and obtains their positions. The obtained positions are used for automatic robot control. Also, the camera recognizes the combinations of the markers attached to the beakers, and determines the selected EPD task among various predetermined EPD tasks with different working parameters. Finally, experimental results are shown for verifying the effectiveness of the suggested EPD robot system.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.18-25
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• 2015

A universal DNA barcoding for agricultural noxious weeds is a powerful technique for species identification without morphological knowledge, by using short sections of DNA from a specific region of the genome. Two standard barcode markers, chloroplast rbcL and matK, and a supplementary nuclear ribosomal Internal Transcribed Spacer (ITS) region were used to examine the effectiveness of the markers for Pooideae barcoding using 163 individuals of 29 taxa across 16 genera of Korean Pooideae. The rbcL and ITS revealed a good level of amplification and sequencing success while matK did not. Barcode gaps were 78.6% for rbcL, 96.2% for matK, and 91.7% for ITS, respectively. Resolving powers were 89.3% for rbcL, 92.3% for matK, and 79.1% for ITS. The matK obtained the best both barcode gap and resolving power. However, it should be considered not to employ matK for Pooideae barcode because of low rate of PCR amplification and sequencing success. As a single DNA marker, rbcL and ITS were reasonable for Pooideae barcode. Barcode gap and resolving power were increased when ITS was incorporated into the rbcL. The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use.

• Cartoon and Animation Studies
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• s.41
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• pp.337-356
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• 2015

Recently augmented reality contents are variously used in public such as advertisements or exhibits as well as children's books. Therefore, it is certain that the market, development of augmented reality contents, is gradually growing. Those who are the producer of augmented reality may be familiar with the skill where those images are used as a marker which is created by image recognition technique. In case of using image recognition technique, they usually use the augmented reality marker platform from Qualcomm since it is able to recognize self-produced images and 3-dimensional figures at no cost. This study was started when undergraduate students began to use those general techniques in their contents producing process. AR majoring students in Namseoul University applied image recognition technique to 3 AR contents exhibited in Sejong Center. Creating 3 different images, they have registered images at Image Target Manager provided by Vuforia to use as a marker. Moreover, they have modified the image producing method to raise the recognition rate by research. The higher recognition rate brings the more stable use of augmented reality contents. To achieve the satisfied rate, they have compared the elements of color contrast, pattern and etc. in the use of platform. Thus, the effective image creation method has been drawn. This study is aiming to suggest the production of stable contents by recognizing smart devices' limitation and producing educational contents. The purpose of this study is to help practically augmented reality contents developers by illustrating the application of augmented reality contents which are based on image recognition technique and also its effectiveness at the same time.

• Journal of Life Science
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• v.21 no.12
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• pp.1659-1665
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• 2011

In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.

• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.33-48
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• 2012

As a scientific curiosity to understand the structure and the function of crops and experimental efforts to apply it to plant breeding, genetic maps have been constructed in various crops. Especially, in the case of Brassica crop, genetic mapping has been accelerated since genetic information of model plant $Arabidopsis$ was available. As a result, the whole $B.$ $rapa$ genome (A genome) sequencing has recently been done. The genome sequences offer opportunities to develop molecular markers for genetic analysis in $Brassica$ crops. RFLP markers are widely used as the basis for genetic map construction, but detection system is inefficiency. The technical efficiency and analysis speed of the PCR-based markers become more preferable for many form of $Brassica$ genome study. The massive sequence informative markers such as SSR, SNP and InDels are also available to increase the density of markers for high-resolution genetic analysis. The high density maps are invaluable resources for QTLs analysis, marker assisted selection (MAS), map-based cloning and comparative analysis within $Brassica$ as well as related crop species. Additionally, the advents of new technology, next-generation technique, have served as a momentum for molecular breeding. Here we summarize genetic and genomic resources and suggest their applications for the molecular breeding in $Brassica$ crop.

• Magazine of the Korea Institute for Structural Maintenance and Inspection
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• v.18 no.4
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• pp.83-88
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• 2014

구조물에 최적화된 센서 배열(Sensor Array)을 수행하는 것은 센서 네트워크 설계에서 중요한 요소이다. 그러나 센서의 설치와 관리는 구조물이 처해 있는 환경이나, 경제성 그리고 센서의 주파수대역의 제한과 같은 다양한 원인으로 인해 매우 어려울 수 있다. 이 논문에서는 일반적인 문제와 환경에서 현재 사용되고 있는 물리적 센서 대용으로 가상 시각 센서(VVS: Virtual Visual Sensor)를 제안하였다. 가상 시각 센서는 설치가 간편하고 경제적이며 관리가 편하다는 큰 장점을 가지고 있다. 이러한 가상 시각 센서의 기본적인 아이디어는 최첨단 컴퓨터 시각 알고리즘과 마커 추출 기법의 적용으로 이루어진다. 이 연구에서는 가상 시각 센서를 이용하여 모드 형태와 주파수를 추출하는데 용이하다는 점을 보여주며 이를 구조물 건전성 모니터링에 적용할 경우 효율적이라는 점을 입증하였다.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2006.02a
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• pp.116-123
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• 2006

생물체의 cDNA를 유리기판위에 고밀도로 첨착 시킨 유전자 칩과 정량적인 방법으로 개별 유전자 발현을 진단 가능한 Real- time PCR (실시간 고분자중합연쇄반응 기술) 기법은 첨단 의학분야와 신약개발 및 독성유전체 연구분야에 활발히 도입되고 있는 기술이다. 본 발표의 첫 번째 부분에서는 유전자칩 에서 얻어진 유전자 발현패턴분석에 기반한 바이오마커 선정 및 real time PCR에 의한 확증 관련 기술 과 유전자칩에서 얻어지는 수많은 데이터를 재정렬 및 다양한 분석기법과 display기술을 활용하여 광범위한 화학물질에 대한 독성효과 분석을 가능하게 해주며, 특정 독성물질에 대한 관련유전자 그룹 발견 및 독성영향에 따른 분류방법에 관한 결과를 발표할 것이다. 또한 바이오마커 활용의 하나로 박테리아세포 기반 바이오센서 제작및 세포칩 개발등에 대한 결과도 추가될 것이다. 두 번째 부분에서는 non-model organism(유전체정보가 확보되지 않은 생물체)인 송사리를 이용하여 새로운 2K 유전자칩을 개발하고, 여기서 각종 화학물질에 대하여 얻어진 수많은 유전자칩 분석 데이타를 활용하여 각각의 화학물질이 보여주는 독성효과를 매우 효과적이고 쉽게 이해할 수 있는 display기술을 개발, 적용함으로써 유전자칩 발현에 기반한 화학물질 독성 screening 및 specificity discrimination을 가능케 하는 예가 발표될 것이다. 이 연구에서 개발한 송사리 유전자칩은 간조직의 RNA를 직접 cDNA화 하는 방식을 취하고 있어 전체 송사리의 유전정보를 필요로 하지 않아 비용 및 효율에서 전체 송사리의 유전정보를 얻는 비용과 노력을 취하지 않고 간에서 발생하는 독성학적 영향 및 유전자의 발현정도를 정밀하고 효율적인 방법으로 얻어 낸다. 현재 2000여개의 cDNA유전자중 50%이상의 유전자가 17베타에스트라디올, 페놀, 노닐페놀, 비스페놀, 감마레이조사, 잔류약품중 이보프란, 다이클로펜악, 농약중의 파라???R, 돌연변이 유발물질 중의 이티비알, 금속류중의 카드뮴을 통해 발현양상과 특정 캐미칼별 발현 특이성이 조사되었고, 이들 유전자는 염기서열 분석을 통해 염기서열이 분석되었으며, 미국 NCBI의 유전자 은행과의 비교를 통해 일부유전자는 새로운 유전자로 밝혀지고 있다. 또한 이 발표에서는 소염진통제계열 의약품인 dichlofenac 이 송사리의 각종 조직에 미치는 독성영향을 Real-time PCR을 이용하여 대표적 스트레스 유전자의 발현에 미치는 영향에 대한 분석 예가 발표될 것이다.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.846-850
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• 2009

본 논문은 픽토그램(Pictogram)의 시각전달 요소를 재해석하고 증강현실 환경에서의 효율적 전달 방법에 대하여 제안한다. 픽토그램은 국경을 넘어 남녀노소 모두가 인지할 수 있어야 함이 규칙이며 불특정 다수라는 사용자들을 위해 정확한 정보를 담고 있는 시각전달 매체로서 공정성, 객관성을 가지고 디자인 되어야 한다. 사용자에게 정보전달 인식을 높이기 위해 표준 픽토그램 과 문화 속에서 발견할 수 있는 행위들을 접목하여 애니메이션으로 제작하고, 이를 다양한 연구분야에서 응용되고 있는 증강현실 (Augmented Reality 또는 AR) 기술에 적용하여 표현하였다. 증강현실은 사용자-콘텐츠 정보전달 매체로서 현실 환경을 기반으로 하는 3 차원공간의 시각정보에 대한 사용자의 인식을 향상시킬 수 있는 방법으로 인간-컴퓨터 상호작용(Human-Computer Interaction)을 제공한다. 본 논문에서 제시된 픽토메이션은 기존 공공장소 어디서나 만날 수 있는 다양한 픽토그램을 패턴화하여 마커로 활용하고 사용자는 현재 널리 보급되고 있는 UMPC 혹은 PDA를 통하여 증강현실 환경에서 제작된 픽토메이션 콘텐츠를 경험해 볼 수 있다. 픽토메이션은 시각커뮤니케이션의 효과를 증가 시킬 수 있고, 정보습득의 주체인 사용자에게 콘텐츠의 종류를 예측할 수 있는 선택의 자유를 준다. 본 논문에서는 멀티미디어디자인 요소를 포함한 콘텐츠와의 실시간 상호작용을 통하여 유희적 요소의 접목을 시도하고 증강현실환경에서 Pictomation을 통하여 사용자들과의 효과적 커뮤니케이션을 위한 방법을 모색하고자 한다.

• The Research Journal of the Costume Culture
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• v.14 no.2
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• pp.310-319
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• 2006

This study intended to compare and analyze marking efficiencies of tailored jacket based on different cloth and production conditions, 'the width of cloth', 'the number of marking pieces' and 'the direction for marking deployment'. The results were as follows. For the two pieces of markers, the efficiency of the cloth width of 110cm was higher than that of 150cm. As the number of markers increased, the efficiency of cloth width of 150cm was higher than that of 110cm. In the case of one-directional deployment, the results obtained was that the efficiency of two markers was higher than that of three markers, while, in the case of bi-directional deployment, the efficiency of three markers was higher than that of two markers. In the case of one-directional deployment for each size, the marking efficiencies were higher in two markers at the cloth width of 110 cm and higher in three markers at the cloth width of 150 cm. Then, in marking efficiencies according to the direction for marking deployment, bi-direction marker was the most efficient marker, followed by one-direction for each size marker and one-direction marker.