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http://dx.doi.org/10.5352/JLS.2011.21.12.1659

Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)  

Chae, Chi-Won (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Dutt, Manjul (Citrus Research and Education Center, University of Florida/IFAS)
Yun, Su-Hyun (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Park, Jae-Ho (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Dong-Hoon (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
Journal of Life Science / v.21, no.12, 2011 , pp. 1659-1665 More about this Journal
Abstract
In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.
Keywords
Bulked segregant analysis; Citrus unshiu; male sterility; 'Satsuma' mandarin;
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1 Christopher, A. M. and J. D. Palmer. 1988. Mitochondrial DNA rearrangements and transcriptional alterations in the male-sterile cytoplasm of ogura radish. Mol. Cell Biol. 8, 1474-1480.
2 Dewey, R. E., C. S. Levings III, and D. H. Timothy. 1986. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44, 439-449.   DOI   ScienceOn
3 Fatta Del Bosco, S., M. Siragusa, L. Abbate, S. Lucretti, and N. Tusa. 2007. Production and characterization of new triploid seedless progenies for mandarin improvement. Scientia Horticulturae 114, 258-262.   DOI   ScienceOn
4 Frost, H. B. 1948. Seed reproduction; development of gametes and embryos, pp. 767-816, In Webber H. J. and L. D. Batchelor (eds.), The Citrus Industry, Vol. 1. University of California Press, Berkley.
5 Guérin, G., E. Lerceteau-Kohler, F. Laigret, P. Roudeillac, and B. Denoyes-Rothan. 2003. Development of SCAR marker linked to dominant gene conferring resistance to Colletotrichum acutatum in strawberry. Acta Hort. 626, 85-91.
6 Gulsen, O., A. Uzun, I. Canan, U. Seday, and E. Canihos. 2010. A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica 173, 265-277.   DOI   ScienceOn
7 Asins, M. J. 2002. Present and future of quantitative trait loci analysis in plant breeding. Plant Breeding 121, 281-291.   DOI   ScienceOn
8 Bannerot, H., L. Boulidard, Y. Cauderon, and J. Tempe. 1974. Transfer of cytoplasmic male sterility from Rhaphanus sativus to Brassica oleracea. Proceedings Eucarpia Meeting Crop Section Cruciferae 25, 52-54.
9 Yamamoto, M., N. Okudai, and R. Matsumoto. 1992. Segregation for aborted anthers in hybrid seedlings using Citrus nobilis ${\times}$ C. deliciosa cv. Encore as the seed parent. J. Japan Soc. Hort. Sci. 60, 785-789.   DOI
10 Yamamoto, M., N. Okudai, and R. Matsumoto. 1992. Study on the inheritance of aborted anthers in citrus using seed parents having aborted anthers. J. Japan Soc. Hort. Sci. 60, 791-797.   DOI
11 Yamamoto, M., R. Matsumoto, N. Okudai, and Y. Yamada. 1997. Aborted anthers of Citrus result from gene-cytoplasmic male sterility. Scientia Horticulturae 70, 9-14.   DOI   ScienceOn
12 Yamamoto, M., Y. Yamada, R. Matsumoto, H. Ikemiya and N. Okudai. 1992. Inheritance of seed number in citrus. Bulletin of the Fruit Tree Research Station 23, 47-56.
13 Yamasaki, A., A. Kitajima, N. Ohara, M. Tanaka, and K. Hasegawa. 2009. Characteristics of arrested seeds in Mukaku Kishu-type seedless Citrus. J. Japan Soc. Hort. Sci. 78, 61-67.   DOI   ScienceOn
14 Shen, D. X., Y. Y. Wang, and L. G. Chen. 1998. Genetic breeding of citrus. pp. 161-166, Beijing Science Press.
15 Paran, I. and R. W. Michelmore. 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85, 985-993.
16 Raza, H., M. M. Khan, and A. A. Khan. 2003. Seedlessness in citrus. Int. J. Agr. Biol. 5, 388-391.
17 Rugienius, R., T. Siksnianas, V. Stanys, D. Gelvonauskiene, and V. Bendokas. 2006. Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1. Agronomy Res. 4, 335-339.
18 Sun, S. J., W. Gao, S. Q. Lin, J. Zhu, B. G. Xie, and Z. B. Lin. 2006. Analysis of genetic diversity in Ganoderma population with a novel molecular marker SRAP. Appl. Microbiol. Biotechnol. 72, 537-543.   DOI   ScienceOn
19 Uzun, A., T. Yesiloglu, Y. Aka-Kacar, O. Tuzcu, and O. Gulsen. 2009. Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Scientia Horticulturae 121, 306-312.   DOI   ScienceOn
20 Vardi, A. and P. Spiegel-Roy. 1978. Citrus breeding, taxonomy and species problems. Proc. Int. Soc. Citriculture 184, 51-57.
21 Matsumoto, R., N. Okudai, I. Oiyama, T. Takahara, M. Yamamoto, K. Asada, D. Ishiuchi, and H. Murata. 1991. New citrus cultivar 'Tsunokaori'. Bulletin of the Fruit Tree Research Station 21, 59-65.
22 Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van de lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, and M. Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res. 23, 4407-4414.   DOI   ScienceOn
23 Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535.   DOI   ScienceOn
24 Xiao, J. P., L. G. Chen, M. Xie, H. L. Liu, and W. Q. Ye. 2009. Identification of AFLP fragments linked to seedlessnes s in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers. Scientia Horticulturae 121, 505-510.   DOI   ScienceOn
25 Michelmore, R. W., I. Paran, and R. V. Kesseli. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. 88, 9828-9832.   DOI   ScienceOn
26 Nakamura, M. 1943. Cytological and ecological studies on the genus Citrus, with special reference to the occurrence of sterile pollen grains. Mem. Fac. Sci. Agr. Taihoku Imp. Univ. 27, 53-159.
27 Nakano, M., H. Nesumi, T. Yoshioka, and T. Yoshida. 2001. Segregation of plants with undeveloped anthers among hybrids derived from the seed parent, 'Kiyomi' (Citrus unshiu ${\times}$ C. sinensis). J. Japan Soc. Hort. Sci. 70, 539-545.   DOI   ScienceOn
28 Nesumi, H., M. Nakano, T. Yoshida, T. Yoshioka, and Y. Ito. 1997. Effect of Plasmon on segregation of male sterile traits in citrus. J. Japan. Soc. Hort. Sci. 66, 164-165
29 Nivison, H. T. and M. R. Hanson. 1989. Identification of a mitochondrial protein associated with cytoplasmic male sterility in Petunia. Plant Cell 1, 1121-1130.   DOI
30 Nishiura, M., T. Shichijo, I, Ueno, M. Iwamasa, T. Kihara, Y. Yamada, T. Yoshida, and T. Iwasaki. 1983. New citrus cultivar 'Kiyomi' tangor. Bulletin of the Fruit Tree Research Station 10, 1-9.
31 Ogura, H. 1968. Studies on the new male-sterility in Japanese radish with special reference to the utilisation of this sterility towars the practical raising of hybrid seeds. Mem. Fac. Agric. Kagoshima Univ. 6, 39-78.
32 Ollitrault, P., F. Luro, and M. Yamamoto. 2007. Seedlessness and ploidy manipulations pp. 197-218, In Khan, I. A. (eds), Citrus Genetics, Breeding and Biotechnology. CAB International UK.
33 Gutierres, S., M. Sabar, C. Lelandais, P. Chétrit, P. Diolez, H. Degand, M. Boutry, F. Vedel, Y. de Kouchkovsky, and R. de Paepe. 1997. Lack of mitochondrial and nuclear- encoded subunits of complex I and alteration of the respiratory chain in Nicotiana sylvestris mitochondrial deletion mutants. Proc. Natl. Acad. Sci. 94, 3436-3441.   DOI   ScienceOn
34 Hernandez, P., A. Martin, and G. Dorado. 1999. Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker- assisted selection of wheat. Mol. Breed 5, 245-253.   DOI   ScienceOn
35 Iwamasa, M. 1966. Studies on the sterility in genus Citrus with special reference to the seedlessness. Bulletin of the Horticultural Research Station 6, 1-81.
36 Jung, G., P. W. Skroch, J. Nienhuis, D. P. Coyne, E. Arnaud-Santana, H. M. Ariyarathne, and J. M. Marita. 1999. Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean. Crop Sci. 39, 1448-1455.   DOI
37 Li, G. and C. Quiros. 2001. Sequence-related amplified polymorphism (SRAP) a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103, 455-461.   DOI   ScienceOn
38 Kolosha, V. O. and I. Fodor. 1990. Nucleotide sequence of Citrus limon 26S rRNA gene and secondary structure model of its RNA. Plant Mol. Biol. 14, 147-161.   DOI   ScienceOn
39 Laver, H. K., S. J. Reynolds, F. Moneger, and C. J. Leaver. 1991. Mitochondrial genome organization and expression associated with cytoplasmic male sterility in sunflower (Helianthus annuus). Plant J. 1, 185-193.   DOI   ScienceOn
40 Levings, C. S. III. 1993. Thoughts on cytoplasmic male sterility in cms-T maize. Plant Cell 5, 1285-1290.   DOI
41 Liu, Y. Z., X. T. Ma, H. Y. Zhang, S. Peng, and X. X. Deng. 2004. Status quo of Ponkan (Citrus reticulata Blanco) fruit quality and quality comparison between several production areas in China. Acta Hortic. Sin. 31, 584-588.
42 Banno, K., H. Ishikawa, Y. Hamauzu, and H. Tabira, 1999. Identification of a RAPD marker linked to the susceptible gene of black spot disease in Japanese Pear. J. Japan Soc. Hort. Sci. 68, 476-481.   DOI
43 Budar, F. and G. Pelletier. 2001. Male sterility in plants: occurrence, determinism, significance and use. C.R. Acad.Sci. Paris, Sciences de la vie Life Sciences 324, 543-550.
44 Cao, Q. Q., H. J. Meng, X. P. Wen, H. L. Yi, and X. X. Deng. 2007. Genetic diversity of male sterile and low fertility germplasm of Citrus revealed using SSR markers. Chin. J. Agr. Biotechnol. 4, 99-104.   DOI   ScienceOn
45 Chase, C. D. 2006. Cytoplasmic male sterility: a window to the world of plant mitochondrial-nuclear interactions. Trends Genet. 23, 81-90.   DOI