• Title/Summary/Keyword: 라포형성

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Analysis of Avidin-biotinylated Liposome Layers on Au Electrode by Quartz Crystal Analyzer (수정진동자를 이용한 Au 표면에서 avidin-biotin 결합 리포좀 막의 형성구조 분석)

  • Song, Seong-Hun;Cho, Hong-Sig;Park, Jong-Won;Kim, Kwang;Nakamura, Chikashi;Yang, Qing;Miyake, Jun;Chang, Sang-Mok
    • KSBB Journal
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    • v.15 no.5
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    • pp.497-500
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    • 2000
  • Liposomes and proteoliposomes, artificial membranes, can interact with many solutes, such as drugs, peptides and proteins. The immobilization of (prot대)liposomes as supramolecular aggregates on gold surfaces have potential applications in nano and biosensor technology. We demonstrated a quartz crystal analyzer (QCA) based method to monitor the construction of multi-layers of unilamellar liposomes based on avidin-biotin binding on gold surfaces using a quartz crystal microbalance (QCM). Thus, the QCA provides an on line and efficient method of detecting the construction of protein membranes, which has applications in biosensing systems.

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Narrative Inquiry on the Actual Experience of a Supervisory Relationship in Korea (수퍼비젼 관계형성 실행경험에 대한 내러티브 탐구)

  • Joo, Eunsun
    • The Journal of the Korea Contents Association
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    • v.17 no.9
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    • pp.619-636
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    • 2017
  • This research presents the relationship building between the supervisor and supervisee through the supervision process in a narrative inquiry. The supervisory relationships of the supervisor A and supervisee B, and the effects they have on the relationship between the two are described in as factual terms as possible. The results imply that genuineness and openness are the core components of relationship. Especially, in the case of a highly hierarchical relationship, where it is often easy for the supervisor to employ hidden tactics, efforts on the part of the supervisor's endeavor to develop unconditional acceptance can be helpful in establishing a more open-minded attitude. As a result, the common view in the literature that attributes relationship hierarchy to cultural factors may need to be reexamined. This research provides evidence that in the case of supervision and training in counseling, establishing an environment of acceptance, trust and rapport can help advance the supervisory relationship to a less hierarchical form.

비대면 창업 멘토링 방식이 멘티의 만족도에 미치는 영향 분석

  • Hwang, Bo-Yun
    • 한국벤처창업학회:학술대회논문집
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    • 2021.04a
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    • pp.79-83
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    • 2021
  • 2020년 2월 중순 이루 코로나 바이러스 2019(Covid-19)로 인하여 정부의 기술창업기업 지원 방식도 전반적으로 기존의 대면 방식에서 비대면 방식으로 전환하여 현재까지 대부분 진행되어 오고 있다. 창업기업에 대한 멘토링은 특히 멘티인 창업자와 멘토가 기존에 알고 있는 관계가 아닌 대부분 처음 만나는 관계의 확률이 높음에 따라 사전에 상호간에 라포(Rapport)가 형성되지 않은 상태에서 멘토링이 이루어진다. 이로 인해 Rapport가 형성이 안된 상태에서는 서로 호감을 느끼거나, 나아가 공감대 형성, 그리고 터놓고 이야기 하여, 서로 간의 대화가 충분히 감정적으로나 이성적으로 이해하기가 어려운 경우가 대부분이다. 이러한 경우에는 충분한 멘토링이 어려울 것으로 예상할 수 있다. 따라서 본 연구는 창업 멘토링의 특수성을 감안해 볼 때 정부 기술창업지원 방식 중 기업의 성과를 높이기 위한 멘토링 과정에서 대면 또는 비대면 방식의 방법 차이가 멘토링 만족도에 영향을 주는 것을 그 목적으로 한다. 본 연구는 창업 기업의 성과를 높이기 위한 중소벤처기업부의 도약패키지 사업 중 2019년과 2020년의 제품 아카데미 사업에 참여한 283명을 대면 방식이었던 2019년 211명과 비대면 방식이었던 2020년 72명의 창업 기업 멘티들의 학습 과정에서 발생하는 배운 내용을 실천하고자 하는 학습 전이 효과 경로에 차이가 발생하는 지에 대하여 분석하였다. 자기 기입 설문 방식으로 인해 발생할 수 있는 동일방법편의를 해결하고자 구조화된 설문지 구성할 때부터 응답자의 일관성 동기를 줄이려고 하였고, 통계분석 단계에서도 다수이 방법으로 측정하는 일반 CFA 모형을 활용하였다. 실증 분석결과 비대면 방식의 창업 멘토링 방식을 학습 전이 효과 경로 결과에 있어서 조절효과가 있음을 유의한 통계적 결과고 확인하고, 사후 검증을 통해 볼 때 등분산 가정이 되지 않은 상황에서 차이가 있음을 통계적으로 유의하게 나타났다.

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A Study on the Effects of Children's Forest Experience Activities on Parent-Child Relationship and Child Attitude Change (유아의 숲체험 활동이 부모와 자녀의 상호 관계 및 태도변화에 미치는 영향)

  • Kang, Young-Sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.413-421
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    • 2018
  • This study is intended to look into the effects of children's forest experience activities on parent-child relationship and child attitude change. To achieve this, a survey was empirically carried out to 185 children. The results were as follows. First, the normality of parent-child relationship before and after forest experience activities showed that the parent-child relationship increased from 3.229 points before participation in forest experience activities to 3.841 points after participation in forest experience activities on average. And the child attitude increased from 3.226 points before participation in forest experience activities to 3.765 points after participation in forest experience activities, which there was a change of mean value before and after forest experience activities in both concepts. Second, the difference in parent-child relationship and child attitude change before and after forest experience activities showed that both parent-child relationship(Z=-10.98, p<.001) and child attitude(Z=-11.05, p<.001) did have significant differences before and after forest experience activities in the significant level of .05. As for the above-mentioned findings, children's forest experience activities improves the parent-child relationship and changes their behaviors outside the kindergarten. Consequently, nature-friendly forest experience education can provide emotional stability for children and help them build a positive rapport between teacher, parent and peer.

Effect of Horticultural Therapy on the Stress and Serum Cortisol of Demented Elders (원예치료가 치매노인의 스트레스 및 혈중 코티졸 호르몬에 미치는 영향)

  • Yun, Suk-Young;Choi, Byung-Jin
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.891-894
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    • 2010
  • This study was conducted in order to examine the effects of horticultural therapy on stress in demented elders, and for this purpose, we analyzed stress-related physiological changes in demented elders under the care of an elderly facility who had not taken medication and consented to blood tests. The horticultural therapy program included only flower decoration, which was preferred by the participants, aiming ultimately to reduce stress. First, a one-to-one session was given for forming rapport and the horticultural therapist had many conversations about plants with the elders. Furthermore, the participants were induced to be absorbed into the horticultural activities, though a short while, so that they might forget troubles. Evaluation was made by measuring subjective stress and the blood cortisol hormone concentration before and after the horticultural therapy and examining differences between them. Subjective stress increased from 12.88 to 17.88 in the control group, but decreased significantly from 13.88 before the therapy to 6.38 after in the experimental group (p = 0.007). When the blood cortisol hormone level was measured as a physiological indicator of stress, it decreased significantly from $11.33{\mu}g{\cdot}L^{-1}$ before the horticultural therapy to $9.85{\mu}g{\cdot}L^{-1}$ after (p = 0.037). These results suggest that horticultural therapy reduces the blood cortisol hormone level and is effective in easing stress.

Preparation and Characterization of PE Liposomes Containing Antibody (항체를 포함하는 Phosphatidylethanolamine 리포좀의 제조와 그 특성)

  • 박성호;신현재양지원최태부
    • KSBB Journal
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    • v.10 no.2
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    • pp.204-211
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    • 1995
  • A target-sensitive liposome was prepared by using a dioleoyl-phosphatidylethanolamine(DOPE) and a palmitic acid coupled antibody(p-IgG). For the preparation of stable PE-liposomes, the key factors such as antibody modification method with palmitic acrid, molar ratio of p-IgG to lipid and the amount of various additives, were examined. The optimum molar ratio of p-IgG to lipid was found to be $2.5{\times}10^{-4}$ and the final concentration of deoxycholate for the stable liposome formation was about 0.09%. Two kinds of target-sensitive liposomes, containing polyclonal anti-SRBC(Sheep Red Blood Cell)-antibody and monoclonal anti-${\beta}$-HCG(Human Chorionic Gonadotropin)-antibody, were successfully prepared. The destabilization of liposomes was examined by measuring the release of calcein entrapped in the liposome vesicles. Calcein was released only when the liposomes were contacted with the specific target cells. The calcein release with non-specific target cells was negligible. From this result, it is clear that p-IgG is indispensible for the maintenance of stable PE-liposome and the calcein release is mainly due to the specific interactions between the liposomes containing antibody and the target cells containing antigen.

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Development of Pedagogical Content Knowledge of Novice Secondary Science Teachers through Collaborative Reflection (초임 중등 과학교사들의 협력적 성찰을 통한 수업 전문성 발달)

  • Shin, Minkyoung;Kim, Heui-Baik
    • Journal of The Korean Association For Science Education
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    • v.42 no.1
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    • pp.77-96
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    • 2022
  • This study investigated how collaborative reflection between novice secondary science teachers promoted the development of teaching professionalism. We intentionally selected research participants who shared sufficient rapport. Data were collected by videotaping the classes taught by participants, pre-talk, post-interviews and nine collaborative reflection processes. All data were transcribed and analyzed. Results indicated that all three teachers showed changes in teaching practice. Minyoung's practice involved a teacher-led lecture, but through collaborative reflection, she could create a learning environment to enhance students' power and ownership in her class. Emphasizing academic rigor, Soyoung used to teach content outside the scope of the curriculum, but through collaborative reflection, she became more considerate of students' understanding. Finally, in Jiyeon's classes inquiry activities and theoretical explanations were separated from each other. However, she repeated her efforts to improve her class after collaborative reflection, allowing students to construct explanations through activities. In this study, three factors that promoted the development of teachers' pedagogical content knowledge through collaborative reflection were identified. First, the different teaching orientations of the three teachers who participated in this study, promoted sharing of opinions through collaborative reflection. Second, reflection based on teaching practice enabled practical feedback on the class, which enhanced the development of teachers' pedagogical content knowledge. Third, the equal status and formation of rapport between the three teachers created an environment for productive reflection. These results suggest that future teacher education programs should target communities that can promote collaborative reflection based on teachers' teaching practice.

An Exploration of the Experience of Compassion Fatigue in Nurses Working with Oncology Patients (암환자를 돌보는 병동 간호사의 공감피로 경험 탐색)

  • Kim, Soo Hyun;Kim, Min Ah
    • Journal of Digital Convergence
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    • v.18 no.11
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    • pp.581-593
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    • 2020
  • This study aimed to understand experiences of compassion fatigue reported by nurses providing services to oncology patients and families. We conducted in-depth interviews with 8 nurses currently working in oncology. Three main themes and 13 subthemes were extracted from the thematic analysis suggested by Braun and Clarke. Participants experienced compassion fatigue while feeling the sufferings transferred from patients and caregivers. The stress resulted from unique nature of oncology work added to their compassion fatigue. Participants became running low on empathy towards oncology patients and caregivers because of external work environments and negative perceptions on compassion. The results of the study inform health care professionals about increasing awareness of compassion fatigue of nurses working with oncology patients and families and the importance of providing interventions to promote their individual and professional quality of life.

The Convergence Effect of Major Satisfaction, Resilience on Clinical Practice Satisfaction of Dental Hygiene Students (치위생(학)과 학생의 전공만족도, 회복탄력성이 임상실습만족도에 미치는 융합적 영향)

  • Lee, Ji-Won;Choi, Ha-Na;Jung, Ui-Jung;Kim, Mi
    • Journal of the Korea Convergence Society
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    • v.12 no.10
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    • pp.113-120
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    • 2021
  • This study was conducted to investigate the degree of major satisfaction, resilience and clinical practice satisfaction of dental hygiene students, and to find out factors affecting clinical practice satisfaction. A survey was conducted for students from the dental hygiene department with clinical practice experience, and a total of 256 questionnaires were finally analyzed. For data analysis, descriptive statistics, t-test, ANOVA, and multiple regression analysis were performed according to the purpose of the study. As a result of the study, it was found that relational of major satisfaction had a significant positive effect on the variables affecting clinical practice satisfaction. Based on these results, in order to improve clinical practice satisfaction, it is important to increase relationship satisfaction as a sub-factor of major satisfaction.

FIXED PROSTHESES AND OVERDENTURE TREATMENT OF A PATIENTS WITH DOWN SYNDROME: CASE REPORT (다운증후군 성인환자의 완전구강회복을 위한 피개 의치와 고정성 보철물 적용치료 : 증례보고)

  • Lim, Kyung-Cheol;Lee, Jae-Young;Kim, Young-Jae;Jin, Bo-Hyoung
    • The Journal of Korea Assosiation for Disability and Oral Health
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    • v.14 no.2
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    • pp.106-110
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    • 2018
  • A 20-year-old male patient with Down syndrome presented with rampant caries and chronic periodontitis on full-mouth. Patient have many oral diseases despite of no abnormalities of medical history, no involuntary tremor, no excessive masticatory pressure, and no limitation of exercise. Also he had no experiences of oral health education of prostheses and oral health management. Therefore, A number of fixed prostheses and denture treatments were performed to caregiver and patient with education for oral hygiene and management. After delivery final prostheses, they had repeated follow-up for checking management.