• 월간산업보건
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• s.358
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• pp.42-46
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• 2018

• Journal of the Society of Cosmetic Scientists of Korea
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• v.23 no.1
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• pp.159-184
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• 1997

인체에 안전하고 유효한 화장품을 개발하고 제품의 질을 평가하기 위하여 표준화된 안전성 및 유효성 시험방법이 필요하며, 기능성 화장품 등 제품의 개발 환경에 맞추어 신규 시험방법의 개발이 요구되고 있다. 또한 유럽에서부터 시작되고 있는 화장품 영역에서의 동물실험을 줄이려는 경향에 따라 동물대체 실험 법도 동시에 요구되고 있는 실정이다. 이에 일반 화장품 및 기능성 제품 개발에 필요한 피부에서의 안전성 및 유효성 시험 법에 대한 현황과 표준화의 필요성에 대한 제안과 새로운 시험법의 개발 전망에 대하여 알아보고, 동물 대체 실험 법에 대한 연구 동향과 전망에 관해 기술하였다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.2
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• pp.129-138
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• 1999

피부 전용 제재 개발을 위해 요구되는 동물 대체 시험법 중 가장 적극적으로 연구가 행하여지곤 실제 실용화가 예상되는 것은 안점막 자극 시험으로 지금까지 여러 가지 방법이 개발되었지만 그 중 계란 유정란의 응모요막(CAM)을 이용한 방법이 현재 가장 활발히 진행되고 있다. 이 방법이 일부 국가에서 이미 안점막 자극 시험 동물 대체 시험법으로 공인되었으며 현재까지도 validation 연구를 활발히 진행하고 있다. 본 연구에서도 국내에 적합한 안점막 자극 시험 동물 대체 시험법의 공인 시험법 개발 및 validation study를 목표로 계란 유정란의 응모요막을 이용한 방법 중 HET-CAM 방법을 시행하였으며 안점막 동물 대체 시험법으로 확립하고자 하였다. 틴ET-CAM 방법의 보완을 위해 배양된 세포를 통해 자극도를 측정할 수 있는 방법인 Cytotoxicity test를 도입하여 시행하였으며 두 방법의 data들을 분석하여 validation study를 수행하였다. 국내 유수의 6개 장업사가 본 연구에 참가하여 20가지의 화장품 전용제재를 대상으로 1차, 2차 validation study 를 진행하였다. HET-CAM test, Draize eye irritation test, Cytotoxicity test 측정 결과 HET-CAM 의 “Q” 수치는 대부분 강자극 수치인 2 이상이었고 10% sodium hydroxide가 가장 높은 수치를 보였으며 Tween 20(sorbitanpolyoxyethylene monolaurate) 100%가 가장 낮은 수치를 보였다. In vi패의 경우 10% sodium hydroxide가 가장 높은 수치를 보였으며 30군 propylene glycol 이 가장 낮은 자극수치를 보였다. HET-CAM test 와 Draize eye irritation test, Cytotoxicity test 간의 상관성 분석은 linear correlation coefficient 와 rank correlation coefficient를 구하여 비교하였으며 6개 장업사(A-F)의 실험실에서의 HET-CAM test 결과를 취합하여 각각 두 실험실간의 상관관계(linear correlation)를 분석하였다. Linear correlation coefficient 분석 결과를 보면 전반적으로 상관관계가 0.589 - 0.954의 범위였으며, 특히 A사와 B사 사이의 경우 0.954이었으며, E사와 D사 사이의 경우 0.942로 높은 상관관계를 보였다. 그 외에도 A사와 D사 사이의 경우(0.589)와 B사와 D사 사이의 경우(0.638)를 제외하고는 대체로 높은 상관관계를 나타내었다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.1
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• pp.9-21
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• 2021

In this study, the difference between the preservative efficacy test (PET) used for water dispersion formulations and PCPC (personal care products council) guidelines alternative test method, direct contact membrane method, and surface mold test should be studied to determine what should be considered during preservative efficacy test of water non-dispersible formulations. We conducted improved PETs when using the alternative test method compared with the test methods used for stick and pressed powder. There was no significant improvement between water-in-silicon emulsions and loose powder using alternative test methods. When we checked the results of the presence and absence of the preservative system for each product, we could see that there were differences in testing methods. As a result, improved levels of results could be obtained using both existing and alternative test methods when measuring preservatives for water non-dispersible formulations. In addition, in the case of stick and pressed powder, the results were more effective when the preservative test method applied to the consumer's method of use was applied.

• International Journal of Highway Engineering
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• v.2 no.2
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• pp.149-161
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• 2000

The stiffness of the subbase materials is represented by the resilient modulus, $M_R$, which are very important properties in the mechanistic design of flexible pavement system. However, the cyclic $M_R$ testing method is too complex, expensive, and time consuming to be applicable on a production basis. In this study, the alternative $M_R$ testing technique for subbase materials was developed using a free-free resonant column (FF-RC) test considering deformational characteristics of subbase materials. To estimate the deformational characteristics of subbase materials, effects of strain amplitude and mean effective stress on modulus of subbase materials were investigated. The $M_R$ values determined by alternative testing procedures matched well with those determined by standard $M_R$ test, showing the capability of the proposed methods being used in determining $M_R$ values.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.4
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• pp.239-250
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• 1993

The dynamic resilient modulus tests to determine the $M_R$ of the soils require expensive equipments and well trained personnels to obtain reliable test results. These problems inherent in the dynamic resilient modulus testing have been realized as major negative factors to hinder the $M_R$ test from being practically implemented as a routine test. In this regard. it is highly desirable to develop a simpler alternative testing method incorporating inexpensive equipments and easy-to-perform testing procedures. Developed in this study is an alternative $M_R$ test method based on statically repeated loading scheme utilizing the standard static triaxial test equipments. Applicability and limitations of the developed static $M_R$ testing method are investigated for typical subgrade soils in Korea.

• The Korean Journal of Pesticide Science
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• v.16 no.3
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• pp.261-266
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• 2012

It is common to use many experiment animals to evaluate the toxicity of chemicals including pesticides. For protecting animal, the concepts of 3R (Reduction, Replacement, Refinement) were introduced and in vitro alternatives methods actively have been developed all over the world. Many experimental animals for toxicological tests have been used, so that it is important to establish the alternative methods. In this study, the alternative method using reconstituted human skin model (Keraskin$^{TM}$) was conducted for classification of skin irritation on pesticides. Sixteen formulations selected on the basis of the degree of irritation were treated by Keraskin$^{TM}$ test. The percent of cell viability was measured into the culture medium collected after treatment of the pesticides for 24-72 hrs. The skin irritations of formulations were evaluated by the cell viability. In this study, The 4 formulations with mild irritation in rabbits were evaluated as nonirritant, the 6 formulations with moderate and severe irritation were evaluated as irritant in human skin model test. We suggest that the alternative test using Keraskin$^{TM}$ model could be used as toxicity evaluation for primary irritation index (P.I.I.) score of greater than or equal to 2.1 of pesticides. The further studies should be required to apply for hazardous assessment of pesticides on alternative skin irritation methods because of the interindividual variability of the sensitivity of skin irritation on pesticides.

• Analytical Science and Technology
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• v.27 no.4
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• pp.196-200
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• 2014

Currently nonaqueous titration method for the assay method using the hazardous reagent, mercury acetate for difemerine hydrochloride has been used in Korean Pharmaceutical Codex. We developed an alternative titration assay method by substituting the use of the hazardous reagent, mercury acetate to the use of less toxic ones like ethyl alcohol. The linearity of the calibration curves in the desired concentration range was good (r>0.999). Precision was less than 0.64%. Accuracy was obtained with recoveries in range of 99.10% and 99.71%. The developed assay could be expected to become valuable tools for revising the Korean Pharmaceutical Codex.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.3 s.52
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• pp.245-251
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• 2005

Safety is one of the key issue in the regulation of cosmetics. Cosmetic Act deals with it in Korea. The guidance for the testing cosmetic ingredients and their safety evaluation are prepared by Korea Food and Drug Administration. Ultraviolet radiation could Induce skin damage, edema, erythema, photoaging, immune dysfunction and skin cancer. Ultraviolet radiation is classified as Group 2A(probably carcinogenic to humans) by International Agenry for Reaserch on Cancer(IARC). The in vitro methodologies for evaluating the toxic potential of ingredients reported in the literature have not yet been sufficiently validated for use in areas other than the study for mutagenicity/genotoxicity, for pre-screening for severe irritancy, for screening of phototoxicity and for evaluating the percutaneous absorption. The 3T3 neutral red uptake photoxicity test (3T3 NRU PT) was accepted as OECD toxicity guideline in 2002. The 3T3 NRU PT is an in vitro method based on a comparison of the cytotoxicitv of a chemical when tested in the presence and in the absence of exposure to a non-cytotoxic dose of UVA/visible light.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.727-736
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• 2017

In this study, the method of evaluation of moisturizing ability of cosmetics using specimens was studied as an alternative method of clinical tests. Cosmetic spreadability, surface drying, and moisture loss rate of four specimens (Japanese cake (Maru mochi), Garaetteok, wheat flour, and agar) were evaluated. Also, the water loss rate of the specimens and the transepidermal waterloss were analyzed for 10 kinds of lip make-up products (5 kinds of lipstick, 3 kinds of lip balm, 2 kinds of lip gloss). The moisture loss rate (moisture evaporation sensitivity) of the agar specimen was highest in the order of agar> Garaetteok> Japanese cake> wheat flour. Agar specimens are the most suitable in terms of spreadability, surface crack, and raw material supply. Containers for the preparation of agar specimens are suitable for plastic materials with low heat transfer, which can produce convex, smooth surface specimens. In the evaluation of moisturizing ability of lip makeup products, there was a strong correlation between the water loss rate and the transepidermal waterloss rate measured with agar specimens. These results show that the proposed method can be used as one of the useful alternative test methods.