• Title/Summary/Keyword: 기간분리

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Changes in quality characteristics of salted Kimchi cabbage and kimchi paste during storage (절임배추 및 김치 양념의 저장기간 중 품질 특성 변화)

  • Song, Hye-Yeon;Cheon, Seon-Hwa;Yoo, SeungRan;Chung, Young Bae;Seo, Hye-Young
    • Food Science and Preservation
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    • v.23 no.4
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    • pp.459-470
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    • 2016
  • This study was conducted to determine the potential use for salted Kimchi cabbage and kimchi paste by analyzing the quality characteristic of kimchi prepared with stored them. Salted Kimchi cabbages were packed with the 2% brine and kimchi paste were stored at $4^{\circ}C$ for 8 weeks, respectively. Kimchi prepared at 0 day and kimchi prepared with stored salted Kimchi cabbage and kimchi paste every week were stored at $4^{\circ}C$ for 8 weeks. The salinity and titratable acidity of salted Kimchi cabbage packed with the brine was lower than control. The results of salinity, pH and moisture contents of kimchi paste did not show any significant differences during storage, however, the titratable acidity was increased. In sensory evaluation, salted Kimchi cabbage packed with the brine showed higher score in overall preference than control. Kimchi paste showed 4.9~6.1 score in overall preference during storage. The titratable acidity of kimchi prepared with stored salted Kimchi cabbage and kimchi paste was lower than control. But, the results of sensory evaluation did not show significant difference among treatments. The study suggested that the use of 2% brine is an effective way for extending shelf-life of salted Kimchi cabbage and Kimchi prepared with stored salted Kimchi cabbage and kimchi paste every week is effective to delay the fermentation but no difference in sensory evaluation.

Quality characteristics of Doenjang manufactured with soybean Koji (콩 코오지를 이용한 된장의 품질 특성)

  • Kim, Do-Yoon;Kwon, Dong-Jin
    • Food Science and Preservation
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    • v.21 no.3
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    • pp.434-441
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    • 2014
  • This study investigated the quality characteristics and sensory evaluation of Doenjang samples made of soybean koji and prepared with Bacillus subtillus 3-B-1 and/or Aspergillus oryzae 6-M-1 isolated from traditional Meju, to replace the koji with wheat flour. Among the quality characteristics of doenjang during its fermentation at $30^{\circ}C$ for 40 days, its moisture decreased by degrees, but its amino-type nitrogen and reducing sugar increased by degrees. The color of the doenjang made of koji and prepared with Aspergillus oryzae 6-M-1 changed more significantly than that of the doenjang with Bacillus subtilis 3-B-1. The viable cell and mold counts increased by degrees during the fermentation. The results of the sensory evaluation of the doenjang made with koji and prepared with Aspergillus oryzae 6-M-1 did not significantly differ from those of the doenjang with Bacillus subtilis 3-B-1 and of the commercial doenjang. These results showed the possibility of preparing doenjang with soybean koji instead of wheat flour koji.

The pathogenicity of scuticocilate Philasterides dicentrarchi isolated from cultured olive flounder, Paralichthys olivaceus (양식넙치, Paralichthys olivaceus에서 분리된 스쿠티카 섬모충 Philasterides dicentrarchi의 병원성)

  • Heo, Moon-Soo;Lee, Young-Don;Lee, Je-Hee;Jin, Chang-Nam;Kang, Hyun-Sil;Lee, Chang-Hoon;Kang, Sun-Kyoung
    • Journal of fish pathology
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    • v.19 no.2
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    • pp.87-97
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    • 2006
  • In previous study, scuticociliate P. dicentrachi was isolated from farmed flounder during the december 2004 to april 2005. Pathogenicity, mortality and infection symptom were studied using 3 cm and 5 cm groups of juvenile flounder. The ciliates were exponentially increased from bottom layer of the experimental tanks, which propagated within 2000 cells/ml (1.4 × 103 cell ㎖-1 ~ 2.5 × 103 cell ㎖-1) after 6 days of inoculation. The middle layer was maintained within 300 cells/ml. Both 3 cm and 5 cm groups were infected with ciliates, mainly 3 cm group showed high mortality during the experimental period. The death of 3 cm group was started from 5 days and showed 95.6% of mortality after 28 days of first inoculation. The control group showed 4.4% of mortality however, we could not observed any ciliates. The death of 5 cm group was started later than 3 cm group after 18 days of first inoculation. The total mortality was 71% during 28 days. No mortality and infection symptoms were observed in the control. We also studied SSU rRNA gene of ciliates which, re-isolated from infected flounder of experimental groups. When SSU rRNA in this study compared with previous data showed that the identified strain of both previous and present study was same.

Characterization of the Quinoline-Degrading Bacterium Pseudomonas sp. NFQ-1 Isolated from Dead Coal Pit Areas (폐광지역에서 분리한 quinoline 분해 세균인 Pseudomonas sp. NFQ-1의 특성연구)

  • 윤경하;황선영;권오성;오계헌
    • KSBB Journal
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    • v.18 no.3
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    • pp.174-179
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    • 2003
  • The bacterium NFQ-1 capable of utilizing quinoline (2,3-benzopyridine) as the sole source of carbon, nitrogen and energy was enriched and isolated from soil samples of dead coal pit areas. Strain NFQ-1 was identified as Pseudomonas nitroreducens NFQ-1 by BIOLOG system, and assigned to Pseudomonas sp. NFO-1. Pseudomonas sp. NFQ-1 was used with the concentration range of 1 to 10 mM quinoline. Strain NFQ-1 could degrade 2.5 mM quinoline within 9 hours of incubation. Initial pH 8.0 in the culture was reduced to 6.8, and eventually 7.0 as the incubation was proceeding. 2-Hydroxyquinoline, the first intermediate of the degradative pathway, accumulated transiently in the growth medium. The highest concentration of quinoline (15 mM) in this work inhibited cell growth and quinoline degradation. Pseudomonas sp. NFQ-1 was able to utilize various quinoline derivatives and aromatic compounds including 2-hydroxyquinoline, p-comaric acid, benzoic acid, p-cresol, p-hydroxybenzoate, protocatechuic acid, and catechol. The specific activity of catechol oxygenases was determined to approximately 184.7 unit/㎎ for catechol 1.2-dioxygenase and 33.19 unit/㎎ for catechol 2,3-dioxygenase, respectively. As the result, it showed that strain NFQ-1 degraded quinoline via mainly orthp-cleavage pathway, and in partial meta-cleavage pathway.

Physiological Characterization of Lactobacillus sp. JK-8 Isolated from Shrimp Aquaculture Pond (새우양식장에서 분리한 Lactobacillus sp. JK-8의 생리적 특성)

  • Chun Jae-Woo;Ma Chae-Woo;Oh Kye-Heon
    • Korean Journal of Microbiology
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    • v.41 no.1
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    • pp.18-23
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    • 2005
  • The purpose of this work was to investigate the physiological characteristics of Lactobacillus sp. JK-8 isolated from a shrimp aquaculture pond. The strain JK-8 was grown on MRS media, and morphological and physiological characteristics of the strain were examined. The bacterium was identified as a strain of the genus Lactobacillus on the basis of BIOLOG test. Strain JK-8 produced both lactic acid and acetic acid, which were responsible for the pH decrease during growth. Concentrations of lactic acid and acetic acid increased to 192.8 mM and 43.6 mM, respectively, and the initial pH 7.0 of the cultures decreased to 3.8 at the end of incubaction. The bacteriocidal effect against eight target bacteria was examined with 5-fold concentrated culture supernatants. All bacteria tested in this work were completely killed within 3 hrs after treatment with the culture supernatant. The bacteriocidal effects were clearly observed, only when the pH of the culture supernatants were not adjusted. HPLC was used to reslove lactic acid and acetic acid in the culture solution, and GC-MS was used to verify the metabolites.

The incremental information content of accruals components of earnings for stock return: Discretionary accruals and non-discretionary accruals (회계이익 구성요소의 추가적 정보가치가 주식수익률에 미치는 영향)

  • Shin, Hyun-Dai
    • The Journal of Information Technology
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    • v.7 no.3
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    • pp.19-36
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    • 2004
  • This study examines the relation between accruals components of earnings and stock return. Earnings are decomposed into four components: discretionary accruals, nondiscretionary accruals, nondiscretionary income and cash flow from operations. Because reported earnings in financial statement consist of cash flow from operations plus total accruals. We decompose total accruals into a discretionary accruals and a nondiscretionary accruals separately. This paper examines the incremental informational content of discretionary accruals and nondiscretionary accruals components of net income by regressing return on earnings' components in multivariate models. The empirical analysis is conducted on a sample of 1,580 firm-years comprising 158 firms during 1991-2003. discretionary accruals are obtained by decomposing total accruals into discretionary and nondiscretionary accruals conponents, using a pooled variation of the Jones model(1991). These findings suggest that the discretionary accruals(measured using a variation the Jones model) is priced by the stock market. Specifically, the discretionary accruals and cash flow from operations are positively associated with the stock return, and also nondiscretionary income, discretionary accruals are positively associated with the stock return. While this result is consistent with the market prices the discretionary accruals because it captures value-relevant information. Additional test report evidence consistent with nondiscretionary accruals conveying information about the stock return.

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Growth Suppression of Microcystis aeruginosa by Pseudomonas aeruginosa AJ1 (Pseudomonas aeruginosa AJ1에 의한 Microcystis aeruginosa의 성장제어)

  • Kim, Sun-Jung;Lee, Sang-Seob
    • Korean Journal of Microbiology
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    • v.45 no.4
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    • pp.362-367
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    • 2009
  • Among total 176 strains with antialgal effects isolated from So-ok stream in Korea, Pseudomonas aeruginosa AJ1 showed the highest removal efficiency for an algal species Microcystis aeruginosa (clear zone of diameter 50.0 mm on algal lawn after 20 days). The algal growth was suppressed even when the supernatant of AJ1 culture was applied, suggesting that extracellular substances are responsible for its antialgal activity. The removal activity of AJ1 was optimal under the following condition: pH 8, $30^{\circ}C$, and mannitol as a carbon source. The antialgal activity of AJ1 appeared to be dependent of the growth phase of M. aeruginosa, i.e., the highest at the early phase, but not its own phase. As expected, the algicidal effect was improved as the amount of the treated supernatant was increased; the highest removal efficiency (80.3%) was achieved when 40 ml/L of the supernatant was used. Interestingly, however, the removal rate was opposite. The highest removal rate ($8.2{\mu}g$ chl-a/ml supernatant/day) was achieved when low concentration (10 ml/L) was applied. These results suggest that P. aeruginosa AJ1 is a promising biological agent to control the problematic algal bloom.

Variation of Species and Nutrient Composition of Benthic Diatoms on Plate during Culture Periods I. Wando area (배양기간에 따른 파판 부착규조류 종조성과 영양성분 변화 I. 완도지역)

  • 김해영;허성범
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.141-142
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    • 2003
  • 본 연구는 전복의 유생사육을 목적으로 먹이생물인 부착미세조류의 파판 배양시 배양기간에 따른 종조성과 영양성분의 변화를 파악하기 위하여 수행되었다. 실험은 완도지역에서 봄철인 2~4월에 8주 동안 실시되었다. 배양을 위한 기초 조사로 배양지역 연안해수의 수온, 염분, pH, 총질소, 총인, Chlorophyll-a를 측정 하였다. 실험에 사용된 파판은 전복 종묘생산에 이용되는 규조배양용 파판과 틀을 이용하여 2주마다 파판을 수거한 뒤 부착된 미세조류를 부드러운 솔로 분리하여 원심분리한 후 지방산과 아미노산을 측정하였다. 각각의 시료는 광학현미경(Nikon)으로 동정한 후 Sedwick-rafter counting chamber를 이용하여 종별로 계수하고 이를 단위면적당 개체수 (cells/$\textrm{cm}^2$)로 환산하여 정량분석을 하였다. 실험 결과 봄철 완도지역의 평균 수온과 염분은 각각 15.4$^{\circ}C$와 33.88$\textperthousand$이었고 pH는 8.21이었다. 총질소와 총인은 각각 0.078mg/$\ell$, 0.018mg/$\ell$였고 chlorophyll-$\alpha$는 1.47$\mu$g/$\ell$ 였다. 부착미세조류의 아미노산 분석 결과는 배양 2주째의 총아미노산 양이 5.63%로 가장 낮았고, 4주는 6.50%, 6주 7.82% 8주 7.87%로, 6주까지 증가하다가 정체하는 경향을 보였다. 필수아미노산의 경우 총아미노산의 경향과 비슷하였고 2, 4, 6, 8주째의 양은 각각 2.75%, 3.16%, 4.01%, 3.96%였다. 필수 아미노산과 비필수아미노산의 비는 2, 4, 6, 8주 각각 0.95, .095, 1.05, 1.01의 결과를 보였다. 지방산의 경우 palmitic acid( $C_{16:0}$)와 palmitoleic acid( $C_{16:1}$)가 43.10~67.66%의 함량을 보였으나 4주째에는 검출되지 않았으며 EPA는 6주에서 10.97%를 보였고 나머지 구간에서는 검출되지 않았다. 부착미세조류의 biomass는 기간별로 2.4$\times$$10^3$ cells/$\textrm{cm}^2$, 7.8$\times$$10^3$ cells/$\textrm{cm}^2$ 25.1$\times$$10^3$ cells/$\textrm{cm}^2$, 44.3$\times$$10^3$ cells/$\textrm{cm}^2$ 이었다. 출현량은 총 64종으로 대부분이 부착규조류였으며 2주, 4주, 6주, 8주 각각 43종, 25종, 19종, 24종의 부착 규조류가 출현하였다. 그리고 우점종은 2주째 Nitzschia petitiana, Fragilia psedonana, 4주 Navicula mollis, Flagilia cylindrus, 6주 Flagilia capucaina, Achnanthes haukiana, 8주 Flagilia islandica, Flagilia cylindrus였다.s였다.

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Electrophoretic Variations in Protein and Some Enzymes during Stratification of Panax ginseng Seeds (인삼종자의 개갑처리과정에 있어서 단백질 및 몇가지 효소의 전기영동특성변이)

  • 최선영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.4
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    • pp.334-341
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    • 1990
  • The electrophoretic variations in protein and some enzymes during stratification of Panax ginsseng seeds were checked in relation to the differentiation and development of immature embryo, Seed-protein on PAGE showed eight major bands during the period of investigation, Four bands of Rf : 0.12, 0.24, 0.66 and 0,72 remained in dark stained condition, whereas the remainders (Rf : 0.40, 0.48, 0.56 and 0,76) became lighter in the staining condition as the treatment period was prolonged, In embryo, which was separately sampled from 75 days after the treatement, the only band of Rf 0.76 was not observed, In peroxidase, the number of bands was gradually increased and came to four bands at 45 days after treatment, and their staining color became gradually darker until the time of final samling. Also, the embryo had the same patterns as those in the intact seed. Esterase showed four bands during the period of investigation. As the treatment period was prolonged, the degree of staining condition came to darker in the band of Rf 0.36, but lighter in the band of Rf 0.56. The band of Rf 0.20 was not detected in the intact seed at 90 days after the treatment, but clearly observed in embryo. Glutamate dehydrogenase had only two bands: one (Rf : 0.16) was detected only at 60 days after the treatment and the other (Rf: 0.14) was always observed throughout the period, and showing simple pattern in the electrophoretic variation. Glutamate-oxaloacetate transaminase showed the only band of Rf 0.36 during the period of investigation and even in embryo, and showing the simplest pattern, indeed.

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Changes in the quality characteristics of kimchi broths and growth of the isolated strains due to blue light emitting diode irradiation (Blue LED (Light emitting diode) 조사에 의한 배추김치와 백김치 발효액의 품질 특성 및 분리 균주의 생육 변화)

  • Oh, Yeong Ji;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.538-545
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    • 2020
  • Kimchi is a widely consumed traditional Korean food, and its probiotic properties have received great attention. In this study, changes in the quality characteristics of fermentation broths obtained from two types of Chinese cabbage kimchi (red with red pepper and white without red pepper) were assessed after the administration of blue light emitting diode (BLED) irradiation at 4℃; characteristics assessed included acidity, chromaticity, antioxidant activity, and growth modulation of isolated microorganisms. The pH of the white kimchi (WK) broth decreased with time; the decrease was delayed significantly under BLED irradiation (p<0.05). BLED irradiation decreased the L (lightness) and b (yellowness) values and increased a (redness) in WK, whereas the a and b values of the red kimchi (RK) broth increased with BLED irradiation. Growth stimulation of lactic acid bacteria by BLED irradiation was observed in both WK and RK. The numbers of yeast and mold were also increased in RK (p<0.05), but not in WK. There was no change observed in the scavenging activities against ABTS (2,2'-azido-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals in both kimchi broths after BLED treatment. The results of this study indicated that BLED irradiation could modulate the fermentation process and the quality characteristics of kimchi during storage.