Electrophoretic Variations in Protein and Some Enzymes during Stratification of Panax ginseng Seeds

인삼종자의 개갑처리과정에 있어서 단백질 및 몇가지 효소의 전기영동특성변이

The electrophoretic variations in protein and some enzymes during stratification of Panax ginsseng seeds were checked in relation to the differentiation and development of immature embryo, Seed-protein on PAGE showed eight major bands during the period of investigation, Four bands of Rf : 0.12, 0.24, 0.66 and 0,72 remained in dark stained condition, whereas the remainders (Rf : 0.40, 0.48, 0.56 and 0,76) became lighter in the staining condition as the treatment period was prolonged, In embryo, which was separately sampled from 75 days after the treatement, the only band of Rf 0.76 was not observed, In peroxidase, the number of bands was gradually increased and came to four bands at 45 days after treatment, and their staining color became gradually darker until the time of final samling. Also, the embryo had the same patterns as those in the intact seed. Esterase showed four bands during the period of investigation. As the treatment period was prolonged, the degree of staining condition came to darker in the band of Rf 0.36, but lighter in the band of Rf 0.56. The band of Rf 0.20 was not detected in the intact seed at 90 days after the treatment, but clearly observed in embryo. Glutamate dehydrogenase had only two bands: one (Rf : 0.16) was detected only at 60 days after the treatment and the other (Rf: 0.14) was always observed throughout the period, and showing simple pattern in the electrophoretic variation. Glutamate-oxaloacetate transaminase showed the only band of Rf 0.36 during the period of investigation and even in embryo, and showing the simplest pattern, indeed.

인삼종자의 개갑처리과정중 종자단백질 및 몇가지 효소의 전기영동변이를 처리단계별로 조사하여 배의 분화 및 발육과의 관계를 검토하였다. 1. 종자단백질에서는 처리종료시까지 8개의 band를 보였는데 Rf 치가 0.12, 0.24, 0.66 및 0.72의 band는 농염인채 거의 변화가 없었으나 나머지 4개의 band는 처리기간이 길어짐에 따라 옅어지는 경향이었다. 한편 처리후 75일부터 분리채취한 배에서는 Rf 치가 0.76인 band를 확인할 수 없었다. 2. Peroxidase는 처리기간이 길어짐에 따라 band의 수가 증가하여 처리후 45일에는 4개의 band를 보였고 그들의 색조는 처리종료시까지 짙어지는 경향이었으며 배에서도 비슷한 양상으로 나타났다. 3. Esterase는 모두 4개의 band를 보였는데 처리일수가 증가함에 따라 Rf치 0.36의 band는 점차 짙어지는 경향이었고 Rf치 0.56의 band는 반대로 점차 옅어지는 경향이었으며 처리후 95일에는 종자(배분이전의 것)에서 소멸되었던 Rf치 0.20의 band가 배에서 선명하게 나타났다. 4. Glutamate dehydrogenase는 처리후 60일에만 나타난 Rf치 0.16의 band와 전조사기간에 걸쳐 나타난 Rf치 0.14의 band만이 확인되어 단조로운 양상을 보였으며 배에서는 후자만이 나타났다. 5. Glutamate-oxaloacetate transaminase는 분리채취한 배를 포함하여 채종시부터 처리종료시까지 Rf 치 0.36의 band가 유일한 것으로서 매우 단조로웠다.