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The pathogenicity of scuticocilate Philasterides dicentrarchi isolated from cultured olive flounder, Paralichthys olivaceus  

Heo, Moon-Soo (제주대학교)
Lee, Young-Don (제주대학교)
Lee, Je-Hee (제주대학교)
Jin, Chang-Nam (제주지방해양수산청)
Kang, Hyun-Sil (제주대학교)
Lee, Chang-Hoon (국립수산과학원)
Kang, Sun-Kyoung (제주대학교)
Publication Information
Journal of fish pathology / v.19, no.2, 2006 , pp. 87-97 More about this Journal
Abstract
In previous study, scuticociliate P. dicentrachi was isolated from farmed flounder during the december 2004 to april 2005. Pathogenicity, mortality and infection symptom were studied using 3 cm and 5 cm groups of juvenile flounder. The ciliates were exponentially increased from bottom layer of the experimental tanks, which propagated within 2000 cells/ml (1.4 × 103 cell ㎖-1 ～ 2.5 × 103 cell ㎖-1) after 6 days of inoculation. The middle layer was maintained within 300 cells/ml. Both 3 cm and 5 cm groups were infected with ciliates, mainly 3 cm group showed high mortality during the experimental period. The death of 3 cm group was started from 5 days and showed 95.6% of mortality after 28 days of first inoculation. The control group showed 4.4% of mortality however, we could not observed any ciliates. The death of 5 cm group was started later than 3 cm group after 18 days of first inoculation. The total mortality was 71% during 28 days. No mortality and infection symptoms were observed in the control. We also studied SSU rRNA gene of ciliates which, re-isolated from infected flounder of experimental groups. When SSU rRNA in this study compared with previous data showed that the identified strain of both previous and present study was same.
Keywords
Philasterides dicentrarchi; scuticociliate; Paralichthys olivaceus;
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