• Title/Summary/Keyword: 근경

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Effect of explant parts and medium components on in vitro regeneration in Osmunda cinnamomea var. forkiensis (꿩고비(Osmunda cinnamomea var. forkiensis) 기내 포자체 재생에 영향을 미치는 배양부위와 배지구성물질)

  • Kwon, Hyuk Joon;Shin, So Lim;Lee, Cheol Hee;Kim, Soo-Young
    • Journal of Plant Biotechnology
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    • v.44 no.4
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    • pp.448-453
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    • 2017
  • This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and $NaH_2PO_4$ concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to $50mg{\cdot}L^{-1}\;NaH_2PO_4$ in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, $50mg{\cdot}L^{-1}\;NaH_2PO_4$, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.

Selection of Angelica gigas Nakai Lines Using Seedling Characteristics (유묘특성(幼苗特性)을 이용한 참당귀(當歸) 계통(系統)의 조기선발(早期選拔))

  • Yu, Hong-Seob;Bang, Jin-Ki;Kim, Young-Guk;Lee, Seung-Tack
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.3
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    • pp.191-195
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    • 1997
  • This study was conducted to obtain the basic information on setting up selection procedure using seedling characteristics in Angelica gigas Nakai lines. Morphological characteristics of the seedlings including height, leaf and petiole length among lines tested showed difference significantly, meaning that earlier selection was possible. Bolting rate at maturity ranged from 0.6 to 74.6% and root yield did from 350 to 3,893kg/ha. Root yield was increased in the lines with below 5% bolting rate. Heritabilities of petiole length, seedling height and leaf length in seedling stage bolting rate and root yield were high, while those of leaf length, root diameter and root length were relatively low. Bolting rate at maturity was positively correlated with seedling height, leaf length, leaf width, petiole length and number of leaves. A highly significant negative correlation was showed between root yield and seedling height, leaf length and width, petiole length, number of leaves, root length or bolting rate. From the above results the selection markers were found to be height, leaf length and petiole length in seedling stage.

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Effect of Soil Characteristics and Fertilizer Application on Fresh Root Yield of Aralia continentalis K. -II. Yield Response to N, P, K Application rates and Nutrient Uptake (독활(獨活)(Aralia continentalis K.) 주산지(主産地) 토양특성(土壤特性)과 시비양분(施肥養分)이 근경수량(根莖收量)에 미치는 영향 -II. 3요소(要素) 시비수준별(施肥水準別) 수량반응(收量反應)과 무기성분(無機成分) 흡수(吸收))

  • Oh, Dong-Hoon;Hwang, Nam-Yul;Na, Jong-Seong;Park, Keon-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.3
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    • pp.209-214
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    • 1994
  • A study was conducted to study yield response and nutrient uptakes with N, P, K application. Its growth and root yield of Aralia continentalis was increased along with the added amount of fertilizer, and optimum fertilization rates of N, P, K were 38, 21, 18kg/10a, respectively. On the path coefficient analysis between the growth characters and root yield, its values showed in the order of plant height, root width, number of budstocks, number of branch, stem width and number of root. Relative yield of non-fertilization against fertilization of N, P, K were low in the order of N, P and K, that is, responses of fertilization on root yield of Aralia continentalis were affected greatly in the order of N, K and P, but the fertilization efficiency was high in the order of K, P and N. Calcium content was the highest and phosphorus content was the lowest among the mineral nutrients contained in the leaf, but relationships between nitrogen content and root yield was showed positive correlation the difference distinctly between the chemicals and the organics.

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Changes in Flavor Compounds of Polygonatum odoratum Root during Roasting (볶음처리에 따른 둥굴레 근경의 향기성분 변화)

  • Park, Nan-Young;Jeong, Yong-Jin;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.39 no.1
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    • pp.99-103
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    • 2007
  • In order to evaluate innate flavor during the roasting process, the components responsible for the aroma of unroasted Polygonatum odoratum root and the volatile odor components released during the roasting process were evaluated using the solid phase-microextraction (SPME) headspace method. The raw P. odoratum root contained aldehyde, alcohol, hydrocarbon and acid components, which gave it a grass-like smell. 2-Methylpyrazine was not revealed from the roasted P. odoratum root at $110^{\circ}C$ and $130^{\circ}C$, but was extracted at $150^{\circ}C$. The 2,5-dimethylpyrazine component was the greatest at $130^{\circ}C$. The hexanal component was greater when the root was roasted at $110^{\circ}C$, compared with those at $130^{\circ}C$ and $150^{\circ}C$. The production of 2-acetyl-1-pyrroline component was the greatest when it was roasted at $130^{\circ}C$. Quantitative descriptive analysis of P. odoratum after roasting revealed a high concentration of 2,5-dimethylpyrazine at $130^{\circ}C$ and $150^{\circ}C$, but a low concentration at $110^{\circ}C$. Most of the 2-acetyl-1-pyrroline, which significantly affected its smell, was revealed at $130^{\circ}C$.

Characteristics of Collected Lines and Effect of Environmental Conditions on Growth of Rehmannia glutinosa Lib. (지황 수집종 특성과 재배환경이 생육에 미치는 영향)

  • Park, Chung-Heon;Park, Chun-Geon;Yu, Hong-Seob;Seong, Nak-Sul;Lee, Bong-Ho;Chung, Rye-Pyo
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.2
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    • pp.138-142
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    • 1999
  • Chinese foxglove (Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the demand for crude drug expands rapidly. This study was conducted to obtain the basic agronomic characteristics and cultivation information of Chinese foxglove. Morphological traits of several Chinese foxglove and their plant growth and yield were investigated under different environmental conditions. The tested lines exhibited clear morphological differences in leaves and roots representing their origins. Rapid root growth and weight increasement occurred in the middle of July. Optimum daylength and temperature conditions were investigated for the adequate plant growth of Chinese foxglove. Root growth was enhanced at $23/18^{\circ}C$ (day/night) with 13 hours daylength condition. Appropriate soil moisture and soil texture were $60{\sim}70%$ and loam soil, respectively.

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Antioxidative and Nitrite Scavenging Activities of Polygonatum odoratum Root Extracts with Different Steaming and Roasting Conditions (둥굴레 근경의 증자 및 볶음조건에 따른 추출물의 항산화성 및 아질산염 소거능 변화)

  • Kim Kyung-Tae;Kim Jung-Ok;Lee Gee-Dong;Kwon Joong-Ho
    • Food Science and Preservation
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    • v.12 no.2
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    • pp.166-172
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    • 2005
  • Response surface methodology (RSM) was applied to monitor the effects of steaming and roasting conditions of Polygonatum odoratum roots an total phenolics content, electron donating ability (EDA) and nitrite-scavenging ability (NSA) of the extract. In steaming and roasting processes based on the central composite design. with variations in steaming time $(60\~180\;min)$, roasting temperature $(110\~150\;min)$ and roasting time $(10\~50\;min)$, coefficients of determinations $(R^2)$ were 0.9356 (p<0.01) in total phenolics, 0.9578 (p<0.01) in EDA and 0.9436 (p<0.01) in NSA (pH 3.0). The maximum value of total phenolics was $2847.67\;mg\%$ at 135.59 min of steaming time, $143.84^{\circ}C$ of roasting temperature and 43.47 min of roasting time. The maximum value of EDA was $75.00\%$ in 108.98 min, $135.56^{\circ}C$ and 48.86 min. The maximum value of NSA (pH 3.0) was $87.38\%$ in 162.80 min, $143.88^{\circ}C$ and 31.97 min, respectively. Total phenolics content of the extract was influenced by heating conditions in the order of roasting temperature, steaming time and roasting time. While EDA and NSA were appreciably influenced by roasting time, followed by roasting temperature and steaming time.

Effects of Tuber Position and Number of Nodes on Growth of Saururus chinensis Baill (삼백초(三白草)의 종근(種根)사용 부위(部位)와 마디수가 근경(根莖) 및 지상부(地上部) 생육(生育)에 미치는 영향(影響))

  • Park, Jae-Ho;Park, Boo-Gyu;Kim, Min-Ja;Park, Seong-Gyu;Kim, Jin-Han
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.4
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    • pp.286-293
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    • 1998
  • This study was carried out to find the effects of seed tuber position and number of nodes on growth of Saururus chinensis Baill. at experimental field of Chungbuk Provincial Rural Development Administration from 1996 to 1997. Seed tubers were divided into upper, middle and lower parts, and they were prepared to 1-, 2-, 3-node cuttings in each. Percentage of sprouting in planting of middle part was highest of 92%, and the percent increased by increasing the number of nodes. Growth of shoot and root tuber was the most favorable with planting of 3-node cuttings of middle part, and leaf area index (LAI) was highest of 3.10 in the same treatment. Dry matter weight and percentage of root tuber were highest of $821g/m^2$ and 28 % with planting of 3-node cuttings of middle part. Yield of dry leaf and root tuber were highest of 272kg/10a and 821kg/10a with planting of 3-node cuttings of middle part.

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Scarification and Gibberellic Acid Affecting to Dormancy Breaking of Variegated Solomon's Seal (Polygonatum odoratum var. pluriflorum 'Variegatum') (파상처리와 지베렐린을 이용한 무늬둥굴레(Polygonatum odoratum var. pluriflorum 'Variegatum')의 휴면타파)

  • Rhie, Yong Ha;Lee, Seung Youn;Park, Ju Hyun;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.296-302
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    • 2014
  • The foliage of variegated Solomon's seal is excellent in cut flower arrangements. However, it has a restricted marketing period because the harvesting is limited in spring and summer. The increased interest requires the year-round production, thus techniques for dormancy breaking and forcing without low temperature treatment is needed. Therefore, experiments were conducted to d etermine whether gibberellic acid (GA) could break dormancy in variegated Solomon's seal. Thes prouting of dormant bud did not occur throughout the experiment when $GA_3$ $400mg{\cdot}L^{-1}$ was applied to dormant rhizomes as a soil drench. However, when plants were treated with a GA drench after scratch with razor blade or were given direct injection of GA, percent sprouting was increased up to 100 or 83.3%, respectively. However, because treatments with razor or syringe may damage internal organs, we tested another method, scarifying the rhizomes with sodium hypochlorite (NaOCl). Rhizome scarification with 4% NaOCl for 6 or 24 hours followed by drench of $GA_3$ $400mg{\cdot}L^{-1}$ increased the dormancy breaking percentage to 70 or 86.7%, respectively. Moreover, scarified and GA-treated rhizomes produced more leaves than untreated or GA-soil drenched plants in the glasshouse. These results showed the possibility of year-round production of variegated Solomon's seal foliage with rhizome scarification and GA treatments.

The Distribution and Life-form of Ferns in Gwangneung Forest (광릉숲 양치식물의 분포와 생활형)

  • Lee, Kang-Hyup;Yeo, Kyung-Taek;Jung, Seung-Sun
    • Korean Journal of Plant Resources
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    • v.19 no.5
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    • pp.592-597
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    • 2006
  • To understand the status of ferns' distribution of Gwangneung forest, flora survey was done during the period of April 2003 to November 2005. The results obtained from this study were as follows: Ferns identified and surveyed were 46 taxa including 9 families 17 genera 43 species 3 varieties were recorded in Gwangneung Forest. Hemicryrtophyte (H) was the most common dormancy from recorded in surveyed area. Epiphyte (E) and Hydatophyte (HH) were calculated as 4.3%, 2.2%, respectively and they were the lower value compared to those of other dormancy form. Consequently, $H-R_{3}-D_{1}-t$ was recorded as the most common life-form of ferns in Gwangneung Forest. Pteridophyta-Quotient (Ptph.-Q.) was calculated as 1.28 and it was the higher value compared to those of other neighboring mountains.

Variation Analysis of Long-term in vitro Cultured Cymbidium goeringii Lindley and Cymbidium kanran Makino (장기간 기내 배양한 춘란(Cymbidium goeringii Lindley) 및 한란(Cymbidium kanran Makino)의 변이 비교)

  • Ryu, Jai-Hyunk;Lee, Hyo-Yeon;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.24 no.2
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    • pp.139-149
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    • 2011
  • RAPD (random amplified polymorphic DNA) analysis was examined to detect variation of in vitro cultured 30 rhizomes of Cymbidium goeringii Lindley and Cymbidium kanran Makino, with long-term (8 years) subculture, respectively. Out of 151 DNA bands detected, the 40 were polymorphic with a polymorphic rate 26.4% in the C. goeringii. Out of 155 DNA bands detected, the 56 were polymorphic with a polymorphic rate 36.1% in the C. kanran. Genetic similarity matrix (GSM) shows from 0.825 to 1.00 with an average of 0.944 in the rhizomes of C. goeringii and 0.812 to 1.00 with an average of 0.913 in the C. kanran. According to the clustering analysis, C. goeringii was divided into 1 group and 2 independent individuals and its structure of clustering was simple than that of C. kanran. The higher polymorphism and the decreased GSM were showed in the long-term in vitro cultured C. goeringii and C. kanran supplemented with growth regulators. The results provide as fundamental data to develop a new materials for plant breeding and resources plant.