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http://dx.doi.org/10.7732/kjpr.2011.24.2.139

Variation Analysis of Long-term in vitro Cultured Cymbidium goeringii Lindley and Cymbidium kanran Makino  

Ryu, Jai-Hyunk (Department of Bioresources Science, Sunchon National University)
Lee, Hyo-Yeon (Faculty of Biotechnology, Jeju National University)
Bae, Chang-Hyu (Department of Bioresources Science, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.24, no.2, 2011 , pp. 139-149 More about this Journal
Abstract
RAPD (random amplified polymorphic DNA) analysis was examined to detect variation of in vitro cultured 30 rhizomes of Cymbidium goeringii Lindley and Cymbidium kanran Makino, with long-term (8 years) subculture, respectively. Out of 151 DNA bands detected, the 40 were polymorphic with a polymorphic rate 26.4% in the C. goeringii. Out of 155 DNA bands detected, the 56 were polymorphic with a polymorphic rate 36.1% in the C. kanran. Genetic similarity matrix (GSM) shows from 0.825 to 1.00 with an average of 0.944 in the rhizomes of C. goeringii and 0.812 to 1.00 with an average of 0.913 in the C. kanran. According to the clustering analysis, C. goeringii was divided into 1 group and 2 independent individuals and its structure of clustering was simple than that of C. kanran. The higher polymorphism and the decreased GSM were showed in the long-term in vitro cultured C. goeringii and C. kanran supplemented with growth regulators. The results provide as fundamental data to develop a new materials for plant breeding and resources plant.
Keywords
C. goeringii Lindley; C. kanran Makino; RAPD; Genetic similarity matrix; Somaclonal variation;
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1 Kishor, R. and H.S. Devi. 2009. Induction of multiple shoots in a monopodial orchid hybrid (Aerides vandarum Reichb.f x Vanda stangeana Reichb.f) using thidiazuron and analysis of their genetic stability. Plant Cell Tiss. Org. Cult. 97(2):121-129.   DOI   ScienceOn
2 Karp, A. 1991. On the current understanding of somaclonal variation. In Oxford Surveys of Plant Molecular and Cell Biology Vol 7. Oxford University Press, New York, USA. pp. 1-58.
3 Kim, B.M. 2004. Analysis of phenotypic and genetic polymorphism of self-pollinated Korean native Cymbidium goeringii seedlings. Department of Horticulture, M.A. Thesis, Seoul Women's Univ. pp. 20-29 (in Korean).
4 Jung, M.S., Y.H. Joung, J.H. Lee, J.K. Choi, K.S. Kim and T.H. Han. 2008. Assessment of genetic diversity of Hedera spp. using RAPD marker technique. J. Kor. Flower Res. 16:28-35 (in Korean).
5 DeVerno, L.L., Y.S. Park, J.M. Bonga and J.M. Barrett. 1999. Somaclonal variation in cryopreserved embryogenic clone of white spruce (Picea glauca Voss). Plant Cell Rep. 18:948-953.   DOI   ScienceOn
6 Garay, L.A. and H.R. Sweet. 1974. Natural and artificial hybrid genetic names of orchid. In C.L. Withner (eds.). The Orchids. Jhon Wiley and Son Press, New York, USA. pp. 485-561.
7 Goto, S.R., C. Thakur and K. Ishii. 1998. Determination of genetic stability in long term microprogated shoots of Pinus thungergii part using RAPD markers. Plant Cell Rep. 18:193-197.   DOI   ScienceOn
8 Zheng, K.L., S. Castiglione, M.G. Biasini, C. Morandi and F. Sala. 1987. Nuclear DNA amplification and genetic mapping. Nucl. Acids Res. 19:303-306.
9 Zhou, T.S. 1995. In vitro culture of Doritaenopsis: comparison between formation of the hyperhydric protocorm-like body (PLB) and the normal PLB. Plant Cell Rep. 15:181-185.   DOI
10 Harn, C. 1973. Orchid. Kor. J. Plant Tissue Cult. 1:40-47 (in Korean).
11 Chung, S.Y. 2003. Molecular phylogenetical relationship of Paphiopedilum and Phragmipedium. Department of Horticulture, Ph.D. Thesis, Seoul Women's Univ. pp. 25-38 (in Korean).
12 Williams, J.G.K., A.R. Kubelik and L.J. Livuk. 1990. DNA Polymorphisms amplified by arbitray primers are useful as genetic markers. Nucl. Acids Res. 18:6531-6535.   DOI   ScienceOn
13 Evans, DA. 1989. Somaclonal variation : Genetic basis and breeding application. Trends in Genet. 5:46-50.   DOI
14 Fnag, G., S. Hammar and R. Grumet, 1992. A quick inexpensive method of removing ploysaccharides from plant genomic DNA. Biotechniques 13:52-55.
15 Yuan, X.F., Z.H. Dai, X.D. Wang and B. Zhao. 2009. Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea in volucrata by RAPD and ISSR markers. Biotech. Lett. 31(8):1279-1287.   DOI   ScienceOn
16 De Melo Ferreira, W., G.B. Kerbauy and A.P.P. Costa. 2006. Micropropagation and Genetic Stability of a Dendrobium Hybrid (Orchidaceae). in vitro Cell. Dev. Bio. Plant 42(6):568-571.   DOI   ScienceOn
17 So, I.S., J.Y. Choi, T.S. Ko and B.J. Oh. 1998. Growth characteristics of a dwarf mutant in Cymbidium kanran and its genetic relationship evaluated by random amplified polymorphic DNA analysis. J. Kor. Flower Res. Soc. 7:11-18 (in Korean).
18 Scowcroft, W.R., R.I.S. Bretell, S.A. Ryan, P.A. Davis and M.A. Pallota. 1987. Somaclonal variation and genomic flux. In Plant Tissue and Cell Culture (eds.). Alan R. Liss, England. pp. 275-286.
19 Chung, J.D., C.K. Chun, S.S. Kim and J.S. Lee 1985. Factors affecting growth of rhizome and organogenesis of Korea native Cymbidium kanran. J. Kor. Soc. Hort. Sci. 26:281-288 (in Korean).
20 Silva, J.A., N. Singh and M. Tanaka. 2006. Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plantlets. Plant Cell Tiss. Org. Cult. 84(2):100119-100128.   DOI   ScienceOn
21 Park, Y.C., M.S. Seong, S.R. Kim and S.W. Song. 2005. RAPD-mediated genetic relationship analysis of Cymbidium. J. Kor. Flower Res. 13:25-30 (in Korean).
22 Raimondi, J.P., R.W. Masuelli and E.L. Camadro. 2001. Assesment of somaclonal variation in aspragus by RAPD fingerprinting and cytogenic analyses. Sci. Hort. 90:19-29.   DOI   ScienceOn
23 Parani, M., A. Anand and A. Parida. 1997. Application of RAPD fingerprinting in selection of micropropagated plants of Piper longum for conservation. Current Sci. 73:81-83.
24 Choi, J.Y., I.S. So, C.H. Pak and B.H. Kwack. 1998. Randomly amplified polymorphic DNA (RAPD) analysis on compatibility of Korean native Cymbidium goeringii with other Cymbidium species. J. Kor. Soc. Hort. Sci. 16:361-363 (in Korean).
25 Peschke, V.M., R.L. Phillips and B.G. Genenbach. 1987. Discovery of transposable element activity among progeny of tissue culture derived maize plants. Science 238:804-807.   DOI
26 Obara-Okeyo, P. and S. Kako. 1998. Genetic diversity and identification of Cymbidium cultivars as measured by random amplified polymorphic DNA (RAPD) markers. Euphytica 99:95-101.   DOI   ScienceOn
27 Bang, J.W., J.H. Park and E.Y. Choi. 1994. Chromosome variation in callus cells derived from different cytogenetic type plants of Scilla scillodes Complex. Kor. J. Plant Tissue Cult. 21:59-63 (in Korean).
28 Broertjes, C. and A.M. Van Harten. 1988. Applied mutation for vegetatively propagated crops. Elsevier Science Publisher, Amsterdam, Netherlands. pp. 225-285.
29 Brown, P.T.H. 1989. DNA methylation in plants and its role in tissue culture. Genome 31:717-729.   DOI
30 Choi, S.H. 2001. Phylogenetic analysis of oriental Cymbidium. Department of Horticulture, Ph.D. Thesis, Seoul Women's Univ. pp. 31-43.
31 Kim, J.Y. and J.S. Lee. 1992. Effect of cultural conditions on rhizome growth and organogenesis of Cymbidium lancifolium native to Korea in vitro. J. Kor. Soc. Hort. Sci. 33:471-476 (in Korean).
32 Bae, C.H., T. Abe, J.I. Lyu, S. Gensaram, Y.H. Shin, H.Y. Lee and S. Yoshida, 2003. Characteristics of plant organism irradiated with heavy-ion beam. Proceed. J. Kor. Plant Biotech. p. 126 (in Korean).
33 Murashige T and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiol. Plant. 15:473-479.   DOI
34 Na, A.S., C.G. Been, K.B. Lim and R.J. Byoung. 2007. Observation of morphological variations and selection of specific RAPD marker for somaclonal variation in Phalaenopsis clones. J. Kor. Flower Res. 15:174-178.
35 Martins, M., D. Sarmento and M.M. Oliveira 2004. Genetic stability of micropropagated almond plantlets, as assesed by RAPD and ISSR markers. Plant Cell Rep. 23:492-496.   DOI   ScienceOn
36 Modgil, M., K. Mahajan, S.K. Chakrabarti, D.R Sharma and R.C. Sobti. 2005. Molecular analysis of genetic stability in micropropagated apple rootstock MM106. Sci. Hort. 104:151-160.   DOI   ScienceOn
37 Lee, H.Y., J.S. Jung and J.S. Lee. 1998. Induction of chlorophyll deficient mutant plant of Cymbidium kanran by EMS treatment. Kor. J. Plant Tissue Cult. 25:183-187.
38 Arditti, J. 1992. Classification and naming of orchids. Fundamentals of Orchid Biology. John Wiley and Sons Press, New York, USA. pp. 55-101.
39 Larkin, P.J. and W.R. Scowcroft. 1981. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197-214.   DOI   ScienceOn
40 Lee, J.S. and B.H. Kwack. 1985. Studies on ecology of Korean native wild orchids. III. Geographical distribution of genus Cymbidium. J. Kor. Soc. Hort. Sci. 26:140-144 (in Korean).