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http://dx.doi.org/10.5010/JPB.2017.44.4.448

Effect of explant parts and medium components on in vitro regeneration in Osmunda cinnamomea var. forkiensis  

Kwon, Hyuk Joon (National Institute of Biological Resources)
Shin, So Lim (National Institute of Biological Resources)
Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
Kim, Soo-Young (National Institute of Biological Resources)
Publication Information
Journal of Plant Biotechnology / v.44, no.4, 2017 , pp. 448-453 More about this Journal
Abstract
This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and $NaH_2PO_4$ concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to $50mg{\cdot}L^{-1}\;NaH_2PO_4$ in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, $50mg{\cdot}L^{-1}\;NaH_2PO_4$, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.
Keywords
Fern; $NaH_2PO_4$; Masspropagation; Pteridophyta; Sporophyte;
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Times Cited By KSCI : 2  (Citation Analysis)
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