• The Korean Journal of Food And Nutrition
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• v.17 no.2
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• pp.156-162
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• 2004

This study was performed as a part of comparative biological studies of carotenoid pigment for the unutilized biological products. The content of total carotenoid in the integument of wild catfish were 0.27(${\pm}$0.7) mg％ and composed of 25.8％ zeaxanthin, 9.7％ diatoxanthin and 9.1 ％ cynthiaxanthin as major carotenoid. The content of total carotenoid in the integument of cultured catfish were 0.07(${\pm}$0.03) mg％ which is relatively lower compare to wild catfish and composed of 48.5％ lutein, 13.9％ zeaxanthin and 13.3％ isocryptoxanthin as major carotenoid. The total carotenoid contents of the slender catfish were 0.75(${\pm}$0.25) mg％ which is relatively higher compare to other species of catfishes. The carotenoids were composed of 24.5％ zeaxanthin, 24.1 ％ 7'8'-dihydro-${\beta}$-carotene-4-ol, 17.9％ 7'8'-dihydro-${\beta}$-carotene and 10.8％ 7'8'-dihydro-${\beta}$-carotene-3-01 as major carotenoid and 8.7％ diatoxanthin, 6.7％ cynthiaxanthin and 5.0％ lutein as minor carotenoid. Based on these data, as a comparative studies of carotenoid in integument of siluridae, parasiloxanthin and 7',8'-dihydroparasiloxanthin which are the characteristic carotenoid of catfish from biwa lake in Japan, Slender catfish contained more based on 7',8'-dihydro-${\beta}$-carotene while that of wild and cultured catfishes were not found, indicating that carotenoid pigment of slender catfish depend on their living conditions.

• Proceedings of the KOR-KST Conference
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• 1993.07a
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• pp.235-247
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• 1993

이십일세기상해항공운수발전전략연구, 시이 $\ll$진흥상해, 개발포동, 복무전국, 면향세계$\gg$ 적방침작위지도, 병통과이상해포동개방개\ulcorner\ulcorner\ulcorner장강삼각주지구내지정개장강류역경제발전, 축보사상해발전성위\ulcorner\ulcorner지 구경제, 김융, 무역중심지일작위목표개전전략연구. 상해재아국두\ulcorner\ulcorner의현대화건설진정중점유겁기중요적지위, 저장성위태평양서안최인적 제무역중심지일, 목전, 상해항공운수적현상여기재국민경제중적전략지 위급여세계경제발달국가급지구례여뉴약, 륜돈, 파려, 지가가, 동경, 향항, 신가파등국제대성시상비차거태대, 안통계수거상해1990년항공운수탄토량약시미국뉴약1986년적1.5%, 시향항적10%좌우, 가견상해민\ulcorner\ulcorner수적 발전속도이경한제료상해경제적등비, 한제료포동개방개발급여국내 외정치경제화문화적교류, 위료사상해항공운수여상해적국민경제급사사회 발전상괄응, 대력발전항공운수기박재미첩. 수착상해경제발전, 능원, 원재료적증가, 외향형경제발전급금후궤십년상해인민수입증가화경제결 구조정.고정첨기출적발전급국제교왕적증가. 예측이십일세기상해여객 운수량급화운량장성배증가, 소이탐색일개여상해경제발전전략목표상괄 응적현대화항공교통운수체계, 발혼항공운수특유적우핵시십분필요적. 본연구보고종이하오개방면대상해이십일세기항공운수발전전략진행천술. 일, 개술발전상해항공운수적필요성급긴박성. 이, 상해민용항공운수현상대상해지구주요승운인동방항공공사항선 망, 운력, 기대, 기장현상작료분석,설명상해민항유우운력불족, 기출 장비체후, 자김결, 불능만족상해경제등비발전적수요. 공중교통관제, 통신도항, 기무유수, 항유공응등방면야도불동정도지한 제료항공운수적발전, 필수인기겁대적중시. 삼, 상해항공운수적예측화전망, 통과자료연구급수거분석, 리용수 학모형대이십일세기상해항공운량진행예측, 제출발전상해항공운수적전 략목표급발전중점. 예측2020년 상해항공항총객운탄토량4300만인/년, 화운량달120만돈; 2050년객운량장달18150만인차/년, 화운량518만돈. 사, 발전상해민항기출경제정책, 제출위료$\ll$진흥상해, 개발포동, 복무전국, 면향세계$\gg$ 화도이십일세기중기국민경제달도중등발달국가수 평굉관전략목표적실현, 제료필수재지도사상상파교통운수진정방도전략 산업지위, 환응재관리체제상채취과단유효적개혁조시, 재기출경제정책 상급여대력부지. 오, 전략목표, 위파상해건설성위태평양서안최대적경제, 김융, 무역적중심, 요구상해항공항성위화동지구통향세계각지항선망출발참, 구성대외개방선면축심, 실현국제항선적함접화국내항반적전항, 형성다축심복사식항선망; 가강기장건설, 개피포동제이국제기장건설, 괄응포동개발경제발전적수요.

• Korean Journal of Microbiology
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• v.41 no.3
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• pp.208-215
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• 2005

This study was accomplished in order to collect fundamental data on microbial roles in recycling process of reed rhizosphere. Sunchon bay, which is considered as one of the marsh and mud environments severely affected by human activities such agriculture and fisheries, was selected as a model place. In our initial efforts, two bacterial consortia were obtained by enrichment culture using PAH mixtures containing anthracene, naphthalene, phenanthrene and pyrene as the sources of carbon and energy, and four pure bacteria capable of rapid degradation of PAH were isolated from them. Four strains designated as SCB1, SCB2, SCB6, and SCB7 revealed by morphological, physiological and molecular analyses were identified as Burkholderia anthina, Alcaligenes sp., Achromobacter xylosoxidans., and Pseudomonas putida, respectively with over $99{\%}$ confidence. Notably, Burkholderia anthina SCB1 and Alcaligenes sp. SCB2 were found to utilize anthracene and pyrene more quickly than naphthalene and phenanthrene, whereas Achromobacter xylosoxidans SCB6 and Pseudomonas putida SCB7 exhibited similar growth and degradation patterns except for pyrene. These facts suggest that the rhizosphere microorganisms capable of PAH degradation might be used to clean up the contamination sites with polycyclic aromatic hydrocarbons.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.90-94
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• 2013

A new Bacillus strain designated as MP56 producing antimicrobial substance has been isolated from the mud flat of Korea. The strain MP56 was found to exhibit broad spectrum of antimicrobial activity against Gram-positive pathogenic microorganisms and MDR (multi drug resistant) strains. The 16S rRNA sequence revealed that the MP56 was closely related to Bacillus subtilis with 99.93% homology. The optimal medium composition for production of antimicrobial substance in the B. subtilis MP56 were 1% mannitol, 1% oat meal, 0.01% $CaCl_2$. Antimicrobial activity of the culture broth against different pathogenic strains was assessed using the antimicrobial spectrum. The result suggests that Bacillus strain MP56 produces high quality antimicrobial substance that might be very useful to control varieties of pathogenic microbial growth.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.149-155
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• 2011

This study was carried out to develop a method of removing unnecessary sugars and high-molecular weight soluble components from water extract by using various highly porous polymer resins: these resins are widely used as adsorbents for polyphenolic compounds. Each anthocyanin-rich fraction (ARF) that was eluted from column packed with 4 different resins (SEPABEADS SP207, 700, 850 and Diaion HP 20) was obtained from an aqueous extract of the ripe fruits of Rubus coreanus, which is well-known in Korea as "Bok-bun-ja". Among the above mentioned resins, Diaion HP20 had the highest efficacy and provided maximum yield of the ARF. The ARF eluted from the column packed with HP 20 exhibited the strongest antioxidant activity in vitro. Bioactive phytochemicals extracted from Rubus coreanus were investigated using the on-line HPLC-$ABTS^{{\cdot}+}$ system, and the resulting 6 peaks were identified as radical-scavenging components. By using liquid chromatography-mass spectrometry, 5 peaks were obtained, and these were identified as cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside and pelargonidin-3-rutinoside. These results indicate that the use of appropriate porous resin (Diaion HP 20) leads to an increase in the yield of bioactive components and enhancement of their biological properties.

• Ocean and Polar Research
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• v.28 no.2
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• pp.95-105
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• 2006

To clarify the seasonal changes of the phytoplankton community in seagrass beds, the abundance and distribution of phytoplankton, and environmental factors were measured in seagrass beds: in the Dongdae Bay and Aenggang Bay on the southern coast of Korea, and off Seungbong Island on the western coast of Korea, in October 2002, January, March, and June 2003. Water temperature, salinity, SPM, chlorophyll a, aboveground biomass of seagrass, DIN and DIP concentrations significantly changed within the sampling time. The taxa of phytoplankton observed in seagrass beds were classified as 3 divisions, 3 classes,4 orders,16 families, 27 genera, 65 species. 50 species of diatoms were recognized with 14 species of dinoflagellates, and 1 species of silicoflagellate. The species of genera Coscinodiscus and Thalassiosira were dominant all around the study areas but Peridinium granii, Eucampia zodiacus and Pleurosigma elongatum were seasonally dominant. Phytoplankton standing crops varied from minimum of $0.6{\times}10^3\;cells\;l^{-1}$ (June, Dongdae Bay) to maximum$21.1{\times}10^3\;cells\;l^{-1}$(March, Aenggang Bay). The standing crops and species composition of phytoplankton were relatively lower and simpler than those of other southern and western coastal areas. Seasonal variations of diatom standing crops in seagrass beds were attributed to seasonal changes in DIN and in DIP of water column.

• Korean Journal of Microbiology
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• v.39 no.1
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• pp.27-35
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• 2003

No currently available methods to monitor pathogenic protozoa, Cryptosporidium and Giardia in environmental water come close to acceptable sensitivity, specificity and reproducibility, and so it has to be accompanied by thorough quality control and performance evaluation to credibly predict the distribution of them. We collected surface water samples from the Han River and spiked our prepared (oo)cysts, determined Matrix Spike recoveries using USEPA Method 1623 and considered what factors influence MS recovery and validity. As a result, average 46% of spiked oocysts and 60% of spike cysts were recovered, but repetitive sampling and statistical approach seemed to be necessary to determine the environmental pollution level of two protozoa as their variation coefficients was so much as 35oio and 26%. And MS recoveries with two acid dissociations during immunomagnetic separation were improved more 10% than that with one dissociations and the use of spiked suspension enumerated by flow cytometry instead of manual preparation enhanced the validity and reliability in spiking tests. Because fluorescence characteristics of (oo)cysts stained on well slides with FITC-labeled monoclonal antibodies and DAPI was not always same, well Elides from spiked field samples were helpful to evaluate the performance of staining. We found many (oo)cyst-like objects with typical fluorescence, not (co)cysts, from the Han River water samples, and then it was concluded that nuclei staining by DAPI (4',6-diamidino-2-phenylindole) and examination by Differential Interference Contrast Microscope should be critical for valid identification.

• Microbiology and Biotechnology Letters
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• v.36 no.4
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• pp.276-284
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• 2008

A lactic acid bacterium having antifungal activity was isolated from kimchi. It was identified as Lactobacillus plantarum based on its morphological and biochemical properties, and 16S rRNA sequence, and designated as Lb. plantarum AF1. This isolate inhibited the growth of Aspergillus flavus ATCC 22546, A. fumigatus ATCC 96918, A. petrakii PF-1, A. ochraceus PF-2, A. nidulans PF-3, Epicoccum nigrum KF-1, and Cladosporium gossypiicola KF-2 under a dual culture overlay assay. Also, the antimicrobial activity was found to be active against various species of Gram-positive and Gram-negative bacteria. The antifungal activity was found to be stable after heat ($121^{\circ}C$, 15 min) and proteolytic enzyme treatment, but it was unstable over pH 5.0. The antifungal compound(s) was estimated to have a low molecular mass (below 3,000 Da).

• Applied Biological Chemistry
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• v.48 no.4
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• pp.418-420
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• 2005

In order to search for biologically active compounds from edible plant sources, the aerial parts of Sajabalssuk (Artemisia herba) were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as eupatilin (1), jaceosidin (2), apigenin (3) and eupafolin (4). Among them, compounds 3 and 4 were isolated for the first time from Sajabalssuk (Artemisia herba).

• Applied Biological Chemistry
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• v.46 no.4
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• pp.376-379
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• 2003

The aerial parts of tile Garland (Chrysanthemum comnarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-ol-3-one$ (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.