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http://dx.doi.org/10.7845/kjm.2013.017

Isolation and Characterization of Bacillus subtilis MP56 with Antimicrobial Activity against MDR (Multi Drug Resistant) Strains  

Park, Sungyong (Department of Pharmacy, College of Pharmacy, Mokpo National University)
Yoo, Jincheol (Department of Pharmacy, College of Pharmacy, Chosun University)
Seong, Chinam (Department of Biology, College of Natural Sciences, Sunchon National University)
Cho, Seungsik (Department of Pharmacy, College of Pharmacy, Mokpo National University)
Publication Information
Korean Journal of Microbiology / v.49, no.1, 2013 , pp. 90-94 More about this Journal
Abstract
A new Bacillus strain designated as MP56 producing antimicrobial substance has been isolated from the mud flat of Korea. The strain MP56 was found to exhibit broad spectrum of antimicrobial activity against Gram-positive pathogenic microorganisms and MDR (multi drug resistant) strains. The 16S rRNA sequence revealed that the MP56 was closely related to Bacillus subtilis with 99.93% homology. The optimal medium composition for production of antimicrobial substance in the B. subtilis MP56 were 1% mannitol, 1% oat meal, 0.01% $CaCl_2$. Antimicrobial activity of the culture broth against different pathogenic strains was assessed using the antimicrobial spectrum. The result suggests that Bacillus strain MP56 produces high quality antimicrobial substance that might be very useful to control varieties of pathogenic microbial growth.
Keywords
Bacillus subtilis; fermentation; MDR (multi drug resistant);
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1 Toama, M.A., Issa, A.A., and Ashour, M.S. 1978. Effect of agar percentage, agar thickness, and medium constituents on antibiotics assay by disc diffusion method. Pharmazie 33, 100-102.
2 Turos, E., Long, T.E., Konaklieva, M.I., Coates, C., Shim, J.Y., Dickey, S., Lim, D.V., and Cannons, A. 2002. N-thiolated beta-lactams: novel antibacterial agents for methicillin-resistant Staphylococcus aureus. Bioorg. Med. Chem. Lett. 12, 2229-2231.   DOI   ScienceOn
3 Woodford, N., Warner, M., and Aucken, H.M. 2000. Vancomycin resistance among epidemic strains of methicillin-resistant Staphylococcus aureus in England and wales. J. Antimicrob. Chemother. 45, 258-259.   DOI   ScienceOn
4 Yoo, H.S., Sohn, M.E., Cho, S.J., Park, S.K., and Lee, S.W. 2007. Characterization of antibacterial substance-producing Bacillus subtilis isolated from traditional Doenjang. J. Kor. Soc. Appl. Biol. Chem. 50, 87-94.   과학기술학회마을
5 Aunpad, R. and Na-Bangchang, K. 2007. Pumilicin 4, a novel bacteriocin with anti-MRSA and anti-VRE activity produced by newly isolated bacteria Bacillus pumilus strain WAPB4. Curr. Microbiol. 55, 308-313.   DOI
6 Chang, H.C. and Yang, E.J. 2007. Characterization of bacteriocin-like substances produced by Bacillus subtilis MJP1. Kor. J. Microbiol. Biotechnol. 35, 339-346.   과학기술학회마을
7 Choi, Y.H., Cho, S.S., Simkhada, J.R., and Yoo, J.C. 2012. A novel thermotolerant and acidotolerant peptide produced by a Bacillus strain newly isolated from a fermented food (kimchi) shows activity against multidrug-resistant bacteria. Int. J. Antimicrob. 40, 80-83.   DOI   ScienceOn
8 Delcambe, L. 1950. Iturine, new antibiotic produced by Bacillus subtilis. C R Seances. Soc. Biol. Fil. 144, 1431-1434.
9 Drago, L., Nicola, L., and De Vecchi, E. 2008. A comparative in-vitro evaluation of resistance selection after exposure to teicoplanin, vancomycin, linezolid and quinupristin-dalfopristin in Staphylococcus aureus and Enterococcus spp. Clin. Microbiol. Infect. 14, 608-611.   DOI   ScienceOn
10 Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783-791.   DOI   ScienceOn
11 Hashizume, H. 2012. Studies for the development of novel anti-MRSA/VRE drugs. Yakugaku zasshi : J. Pharm. Soc. Jap. 132, 59-67.   DOI
12 Kluge, B., Vater, J., Salnikow, J., and Eckart, K. 1988. Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332. FEBS Lett. 231, 107-110.   DOI   ScienceOn
13 Jukes, T.H.a.C.R.C. 1969. Evolution of protein molecules. Mammalian protein metabolism, pp. 21-132. In Munro, H.N. (ed.). Academic Press, New York, N.Y., USA.
14 Kim, D.H., Kang, K.R., Kim, H.W., Yoon, S.Y., Kim, C.G., Yamaguchi, T., Sohng, J.K., and Kang, J.S. 2010. Structure determination of macrolactin compounds with antibacterial activities isolated from Bacillus polyfermenticus KJS-2. J. Life Sci. 20, 1792-1800.   과학기술학회마을   DOI   ScienceOn
15 Klein, C., Kaletta, C., and Entian, K.D. 1993. Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system. Appl. Environ. Microbiol. 59, 296-303.
16 Long, K.S. and Vester, B. 2012. Resistance to linezolid caused by modifications at its binding site on the ribosome. Antimicrob. Agents Chemother. 56, 603-612.   DOI
17 Moshafi, M.H., Forootanfar, H., Ameri, A., Ameri, A., Shakibaie, M., Dehghan-Noudeh, G., and Razavi, M. 2011. Antimicrobial activity of Bacillus sp. strain FAS1 isolated from soil. Pak. J. Pharm. Sci. 24, 269-275.
18 Nakano, M.M. and Zuber, P. 1990. Molecular biology of antibiotic production in Bacillus. Crit. Rev. Biotechnol. 10, 223-240.   DOI
19 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
20 Seo, D.C., Ko, J.A., Gal, S.W., and Lee, S.W. 2010. Characterization of Bacillus lichemiformis KJ-9 isolated from soil. J. Life Sci. 20, 403-410.   DOI   ScienceOn
21 Alanis, A.J. 2005. Resistance to antibiotics: Are we in the post-antibiotic era? Arch. Med. Res. 36, 697-705.   DOI   ScienceOn