Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Deveolopment of Biologically Active Compounds from Edible Plant Sources-VI - Isolation of Sterol Compounds from the Aerial Parts of Garland (Chrysanthemum coronarium L.) -

식용식물자원으로부터 활성물질의 탐색-VI - 쑥갓(Chrysanthemum coronarium L.)으로부터 sterol의 분리 -

  • Song, Myoung-Chong (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Hong, Yoon-Hee (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Kim, Dong-Hyun (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Kim, Dae-Keun (Department of Pharmacy, Woosuk University) ;
  • Chung, In-Sik (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Lee, Youn-Hyung (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Kim, Sung-Hoon (Graduate School of East-Weat Medical Science, KyungHee University) ;
  • Park, Mi-Hyun (Erom Life Co. Ltd.) ;
  • Kim, In-Ho (Korea Food Research Institute) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience & Biotechnology) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University)
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 홍윤희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김동현 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김대근 (우석대학교 약학대학) ;
  • 정인식 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이윤형 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 박미현 ((주)이롬라이프) ;
  • 김인호 (한국식품개발연구원) ;
  • 권병옥 (한국생명공학연구원) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2003.11.30
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Abstract

The aerial parts of tile Garland (Chrysanthemum comnarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-ol-3-one$ (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.

쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. EtOAc 분획으로부터 silica gel column chromatography를 반복하여 3종의 sterol을 분리하였다. 각각의 화학구조는, NMR및 MS등의 스펙트럼을 해석하여, $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-3-one$ (3)으로 동정하였다. 화합물 2와 3은 육상식물에서는 처음으로 분리되었다.

Keywords

References

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