• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.463-467
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• 2009

Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.468-473
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• 2009

Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used for chromosome analysis of hybrids (2n=16) between onion (Allium cepa L., 2n=2X=16) and welsh onion (A. fistulosum L., 2n=2X=16). 5S rDNA, 45S rDNA, and tandemly repeated DNA (TSD) sequence were used as probes for FISH analysis. A. fistulosum specific DNA probe of telomeric repeats and A. fistulosum DNA were used for GISH analysis. In the analysis of meiotic chromosome GISH revealed that hybrids have 7 bivalants and 2 univalents chromosome and 2 univalents were derived from A. fistulosum chromosomes. In somatic chromosomes of hybrid each 8 chromosomes were derived from A. cepa and A. fistulosum, respectively. FISH signal of 45S rDNA probe in A. fistulosum was detected at secondary constriction of chromosomes, while FISH signal in A. cepa was observed in both secondary constriction and telomere of chromosomes. TDS signals in A. fistulosum chromosomes were detected at all subtelomeric of 8 chromosomes and also in 2 pericentromeric of the chromosomes, whereas TDS signals in A. cepa were observed only in subtelomeric in all chromosomes. The pattern of TDS signal in hybrid chromosomes was similar to those of A. fistulosum chromosomes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.353-358
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• 1994

Grain yields of errect-leaved corn hybrids were reported to be increased as planting density(PD) increased compared to those of horizontal leaf type hybrids. This trial was conducted to investigate the difference between errect and horizontal-leaved hybrids in response to different PD. Grain yields of both type hybrids were decreased at the highest PD, however, that of horizontal-leaved hybrid, Ga209 ${\times}$ Ki14A, was decreased more than errect-leaved hybrids which indicated varietal difference in response to PD. Responses to PD for days to tasseling, plant height, ear height, and leaf angle were not significant, also, PD ${\times}$ hybrid interactions were not observed. Yield components were decreased as PD increased and there were no PD ${\times}$ hybrid interactions for these characters. Leaf angle was negatively correlated with grain yield and yield components except for kernel weight.

• The Korean Journal of Zoology
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• v.24 no.4
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• pp.201-215
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• 1981

In vitro intra- and interspecific molecular hybridizations of lactate dehydrogenase isozymes from six species were performed by freezing and thawing the isozymes in sodium chloride, resulting each of the interspecific hybridization of Fluta alba $H_4 \\times$ Ophicephalus argus $M_4$ and that of O. argus $M_4 \\times$ Parasilurus asotus isozymes showed one hybrid isozyme considered to be an $M_2H_2$ isozyme. The results fit with the suggestion that the isozyme of lower vertebrates is a dimer of homodimer as far as te assemblies of subunit M and subunit H are concerned.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.257-262
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• 1997

Intergeneric crosses over 34 cross combinations between genus Brassica and Raphanus were made. Ovules taken out of crossed were cultured on MS media supplemented with 1.0 mg/L GA and BA. Germination of embryo from ovule culture was not influenced by BA and GA in medium but by parental characters in its cross combination. Use of Raphanus sativus cv. Daibyosobudore and Jungkukcheongpi showed low embryo germination when they were used as male part. Cross combination with Brassica juncea as male parent showed slightly increased germination compared to other cross. These results indicated that embryo germination in ovule culture was not much influenced by casein hydrolysate, malt extract, BA, kinetin and glutamine in the medium, but parents in combination were key factor for increasing embryo germination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.1
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• pp.21-25
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• 1978

Primary triticale was newly established by colchicine treatment of intergeneric hybrid F 1 plants crossed between T. aestivum var. Jugoku #81 and S. cereale var. Jaelae. The agronomic and cytological characteristics were studied.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.47-51
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• 1984

Hybrids among some southern pines, Pinus rigida, Pinus taeda and Pinus serotina, were tested in Chunbuk area in order to investigate their adaptability to Korean climate and soils. The results were summarized as follows; 1) Hybrids between P. rigida and P. raeda showed good growth among interspecific hybrids of southern pines tested. In particular, P. taeda${\times}$P. rigida showed remarkable growth in Chunbuk area. 2) The survival rate of P. taeda was extremely low, probably because of cold susceptibility. 3) Volume growth was correlated with width and length of cone, length of needle and needle sheath at the 1% level. 4) Hybrids were generally located between the parents in cluster analysis.

• Korean Journal of Agricultural Science
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• v.33 no.1
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• pp.67-72
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• 2006

This study was carried out to compare a yield performance about CNU maize hybrids developed at the Maize Genetics and Breeding Lab. in Chungnam National University. Among the hybrids incorporated in the test, CNU 3 and 1 showed higher dry matter per 10a and lower kernel yield per 10a than check hybrid, Kwanganok. Hongchon 5 and Suwon169 showed higher kernel yield than Kwanganok. The average yield of CNU 3 hybrid in dry matter and CNU 4 in kernel yield were 2,106kg/10a and 854kg/10a in regional adaptability test, which were increased 33% and 8% compared with a check hybrid, respectively. Also, these hybrids had better stay green characteristics than check hybrid and showed moderately resistance to corn borer and leaf spot.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.45-48
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• 2006

The characteristics of plantlets redifferentiated from calli of F1 hybrid between Panax ginseng and Panax quinquefolius were investigated. Growth of plantlets redifferentiated from F1 hybrid was superior to the plants redifferentiated from Korean ginseng. Stem color of plantlets redifferentiated from F1 hybrid was more purple than that from Korean ginseng and leaf color of the former was also greener than that of the latter. Chunpoong, Yunpoong and Seonweon which are belonged to Korean ginseng showed same PCR band(A), while American ginseng showed different PCR band (B) in Internal Transcribed Spacer (ITS) region. F1 hybrid exhibited both A and B PCR band which belonged to Korean ginseng and American ginseng, respectively. F1 hybrid calli and plantlets redifferentiated from F1 hybrid calli showed same PCR band with that of F1 hybrid plant in ITS region. Therefore it was confirmed that piantlets redifferentiated from F1 hybrid exhibited genetic stability in ITS region.

• Korean Journal of Agricultural Science
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• v.29 no.2
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• pp.1-4
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• 2002

Stem height of the new developed "Daedekchals" waxy hybrids were higher than check hybrid, Chalok #1, while days to tasseling were delayed about four to seven days. Number of tillers per plant of the these new hybrids including check were appeared to be one less as non-effective tiller. Ear length and diameter of the new waxy hybrids were longer and thicker than check, respectively. In addition, pericarp thickness of the Daedekchal #1 and #2 among new developed waxy hybrids were thinner and sugar content were higher than check.