Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Characteristics of Plantlets Redifferentiated from F1 Hybrid between Panax ginseng and Panax quinquefolius

고려인삼과 미국삼의 종간잡종으로부터 재분화된 식물체의 특성

  • Published : 2006.03.01
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Abstract

The characteristics of plantlets redifferentiated from calli of F1 hybrid between Panax ginseng and Panax quinquefolius were investigated. Growth of plantlets redifferentiated from F1 hybrid was superior to the plants redifferentiated from Korean ginseng. Stem color of plantlets redifferentiated from F1 hybrid was more purple than that from Korean ginseng and leaf color of the former was also greener than that of the latter. Chunpoong, Yunpoong and Seonweon which are belonged to Korean ginseng showed same PCR band(A), while American ginseng showed different PCR band (B) in Internal Transcribed Spacer (ITS) region. F1 hybrid exhibited both A and B PCR band which belonged to Korean ginseng and American ginseng, respectively. F1 hybrid calli and plantlets redifferentiated from F1 hybrid calli showed same PCR band with that of F1 hybrid plant in ITS region. Therefore it was confirmed that piantlets redifferentiated from F1 hybrid exhibited genetic stability in ITS region.

교잡 1세대의 화서조직에서 재분화된 F1 유식물체는 고려인삼 유식물체에 비하여 지상부와 지하부 생육이 모두 양호하였으며, 줄기 색도 고려인삼 재분화 식물체의 줄기에 비하여 자색을 강하게 띄었으며 잎의 색도 진한 녹색을 나타내었다. 천풍, 연풍, 선원 등의 고려인삼의 품종 내에서는 Internal Transcribed Spacer (ITS)영역의 DNA PCR 패턴간에 차이점이 나타나지 않았으나, 고려인삼과 미국인삼은 각기 다른 PCR 패턴을 보였으며, 고려인삼과 미국인삼간의 교잡 1세대는 고려인삼과 미국인삼에 나타나는 고유한 PCR패턴을 모두 나타내었다. 교잡 1세대에서 유기한 캘러스와 재분화식물체는 조직배양 모본인 교잡 1세대와 동일한 PCR 패턴을 보임에 따라 교잡 1세대의 조직배양체는 ribosomal DNA의 ITS영역에서 유전적인 안정성을 나타내는 것으로 확인되었다.

Keywords

References

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