Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Variation of Nuclear DNA Content in Interspecific Allium cepa L.×A. fistulosum L. hybrids and Their Successive Backcross Lines

양파와 파간의 종간잡종 F1과 여교잡계통의 핵 DNA 함량

  • Kim, Cheol-Woo (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hwa-Young (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Eul-Tai (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, In-Hu (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Bang, Jin-Ki (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
  • 김철우 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 김화영 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 이을태 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 최인후 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 방진기 (농촌진흥청 식량작물과학원 바이오에너지작물센타)
  • Received : 2009.11.27
  • Published : 20091200
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Abstract

Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

Allium 속 근연종인 파와 양파간 종간교잡을 이용하여 양파로부터 새로운 형질을 도입하는 과정에서 유래되는 종간교잡 F1과 여교잡 세대들의 게놈크기의 변화를 flow cytometry를 이용하여 측정한 결과 다음과 같은 결과를 얻었다. 1. 양파와 파의 종간교잡 F1의 식물학적 특성은 양친의 중간형을 보였으나 여교잡이 진전됨에 따라 반복친인 파의 표현형이 우세하였다. flow cytometry를 이용하여 2C nuclear DNA content을 측정한 결과 $F_1$은 양친에 비해 오히려 증가하였으나 여교잡이 진행됨에 따라 급속히 감소하여 $BC_2F_1$에서는 반복친과 비슷한 수준으로 감소하였다. 여교잡세대의 자식인 $BC_1F_2$은 반복친과 비슷한 $23.1{\pm}1.0pg$으로 측정되었으나 $BC_2F_2$는 $22.9{\pm}0.4pg$으로 반복친의 2C nuclear DNA content인 $23.2{\pm}0.2pg$ 보다 오히려 감소하였다.

Keywords

References

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