한국육종학회지 (Korean Journal of Breeding Science)

  • 제41권4호
  • /
  • Pages.468-473
  • /
  • 2009
  • /
  • 0250-3360(pISSN)
  • /
  • 2287-5174(eISSN)
한국육종학회 (The Korean Society of Breeding Science)
권호 표지

GISH와 FISH를 이용한 양파와 파간 종간교잡계통의 염색체 분석

Chromosome Analysis Using GISH and FISH of Interspecific Hybrids between Allium cepa L. and A. fistulosum L.

  • 김철우 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 이을태 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 김화영 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 최인후 (농촌진흥청 원예특작과학원 목포출장소) ;
  • 방진기 (농촌진흥청 식량작물과학원 바이오에너지작물센타) ;
  • 구달회 (충남대학교 생물과학부) ;
  • 방재욱 (충남대학교 생물과학부)
  • Kim, Cheol-Woo (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Eul-Tai (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hwa-young (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, In-Hu (Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Bang, Jin-Ki (Bioenergy Crop Research Center, NICS, RDA) ;
  • Koo, Dal-Hoe (College of Bioscience and Biotechnology, Chungnam National University) ;
  • Bang, Jae-Wook (College of Bioscience and Biotechnology, Chungnam National University)
  • 투고 : 2009.11.27
  • 발행 : 20091200
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 양파와 파간 종간교잡 F1의 불임성의 한 요인으로 여겨지는 염색체 이상과 교잡식물체의 염색체의 조성을 밝혀 향후 종간교잡을 이용한 양파의 형질개량과정에 종간교잡 육종의 효율성을 높이고자 하였다. 종간교잡식물체의 감수분열 염색체를 관찰한 결과 중기에 7쌍의 2가염색체와 2개의 1가염색체가 관찰되었다. 체세포 염색체수는 16개로 양파, 파의 염색체 수와 같았으며 GISH한 결과 파와 양파의 염색체가 각각 8개씩 확인되었다. 5S, 45S, TSD DNA를 probe로 하여 양파, 파, 종간잡종의 염색체의 signal을 관찰한 결과 5S와 45S rDNA는 는 3종 모두에서 1쌍의 염색체에서 나타났는데 5S의 경우 염색체의 subcentromeric 에서 45S는 부수체 염색체에서 관찰되었다. TSD DNA는 염색체의 telomeric 부위에서 관찰되었으나 양파의 경우 2쌍은 염색체의 말단부와 중심체 부분에서 signal이 관찰되었고 종간교잡 세포에서도 2쌍이 관찰되어 양파에서 유래된 염색체임이 확인되었다.

Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used for chromosome analysis of hybrids (2n=16) between onion (Allium cepa L., 2n=2X=16) and welsh onion (A. fistulosum L., 2n=2X=16). 5S rDNA, 45S rDNA, and tandemly repeated DNA (TSD) sequence were used as probes for FISH analysis. A. fistulosum specific DNA probe of telomeric repeats and A. fistulosum DNA were used for GISH analysis. In the analysis of meiotic chromosome GISH revealed that hybrids have 7 bivalants and 2 univalents chromosome and 2 univalents were derived from A. fistulosum chromosomes. In somatic chromosomes of hybrid each 8 chromosomes were derived from A. cepa and A. fistulosum, respectively. FISH signal of 45S rDNA probe in A. fistulosum was detected at secondary constriction of chromosomes, while FISH signal in A. cepa was observed in both secondary constriction and telomere of chromosomes. TDS signals in A. fistulosum chromosomes were detected at all subtelomeric of 8 chromosomes and also in 2 pericentromeric of the chromosomes, whereas TDS signals in A. cepa were observed only in subtelomeric in all chromosomes. The pattern of TDS signal in hybrid chromosomes was similar to those of A. fistulosum chromosomes.

키워드

참고문헌

  1. Emsweller SL, Jones HA. 1935. An interspecific hybrid in Allium. Hilgardia. 9:265-273
  2. Friesen N, Klaas M. 1998. Origin of some minor vegetative propagated Allium crops studies with RAPD and GISH. Genetic Resources and Crop Evolution 45:511-523 https://doi.org/10.1023/A:1008647700251
  3. Fukui K, Kamisuqi Y, Saki F 1994. Physical mapping of 5S rDNA loci by directed cloned biotinylated probes in barly chromosomes. Genome 37:105-111 https://doi.org/10.1139/g94-013
  4. Hou A, Peffley EB. 2000. Recombinant chromosomes of advanced backcross plant between Allium cepa L. and A. fistulosum L. revealed by in situ hybridization. Theor. Appl. Genet. 100:1190-1196 https://doi.org/10.1007/s001220051423
  5. Jones RN, Rees H. 1968. Nuclear DNA variation in Allium, Heredity 23, 591-605 https://doi.org/10.1038/hdy.1968.76
  6. Khrustaleva LI, Kik C. 1998. Cytogenetic studies in the bridge cross Allium cepa×(A. fistulosum×A. roylei). Theor. Appl. Genet. 96:8-14 https://doi.org/10.1007/s001220050702
  7. Koo DH, Kim SY, Bang KH, Seong NS, Band JW 2003. Cytogenetic analyses of Angelica plants using Feulgen staining and multicolor fluorescence in situ hybridization. Korean J. Plant Biotech. 30:123-127 https://doi.org/10.5010/JPB.2003.30.2.123
  8. Levan A 1936. Die zytologie von Allium cepa and A. fistulosum. Hereditas 21:195-214 https://doi.org/10.1111/j.1601-5223.1936.tb03199.x
  9. Maeda T. 1937. Chiasma studies in Allium fistulosum, Allium cepa and their F1, F2, and backcross hybrids. Jpn Jour of Genet 13:146-159 https://doi.org/10.1266/jjg.13.146
  10. Peffley EB. 1986. Evidence for chromosome difference of Allium fistulosum and A. cepa. J. Am. Soc. Hort. Sci. 111:126-129
  11. Peffley EB, Hou A. 2000. Bulb-type onion introgression possessing Allium fistulosum L. genes recovered from interspecific hybrid backcross between A. cepa L. and A. fistulosum. Theor. Appl. Genet. 94:465-471 https://doi.org/10.1007/s001220050438
  12. Peterka H, Budahn H, Schrader O. 1997. Intespecific hybrids between onion(Allum cepa L.) with S-cytoplasm and Leek (A. ampeloprasum L.).Theor. Appl. Genet. 94:383-389 https://doi.org/10.1007/s001220050427
  13. Ricroch A, Peffley EB, Baker RJ. 1992. Chromosomal location of rDNA in Allium: in situ hybridization using biotin- and fluorescein-labeled probe. Theor. Appl. Genet. 83:413-418 https://doi.org/10.1007/BF00226528
  14. Ulloa-G M, Corgan JN, Dunford M. 1994. Chromosome characteristics and behavior differences in Allium fistulosum L., A. cepa L., their F1 hybrid and selected backcross progeny. Theor. Appl. Genet. 89:567-571 https://doi.org/10.1007/BF00222449
  15. Vosa G. 1976. Hetrochromatic patterns in Allium I. The relationship between the species of the Cepa group and its allies. Heredity 36: 383-392 https://doi.org/10.1038/hdy.1976.45