• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.575-579
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• 2004

4-Geranyloxy compound (1) has elucidated by spectroscopic analysis. This compound (1) inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (2 mm inhibition zone at 15 ㎍/disc), cytotoxic to P388 murine leukaemia eel/lines ATCC CCL 46 P388D1, (IC/sub 50/ 1,125 ng/ml at 7.5㎍/disc) and BSC monkey kidney cell lines (100% of well at 15 ㎍/disc).

• Journal of Life Science
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• v.11 no.2
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• pp.99-102
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• 2001

Mouse malignant T-lymphoma CS21 cells can grow when cocultured with CAl2 lymph node stromal cells, but they undergo apoptotic cell death with DNA fragmentation when separated from CA12 stromal cells. In the course of examining the effects of the soluble factor (s) secreted by CAl2 stromal cells on CS2l cell growth, we found that thiols including cysteine promoted CS2l cell growth. P388 cell growth was also promoted by various thiols. These results suggest that thiols including cysteine participate in CA12 and P388 cell growth.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1290-1294
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• 2006

Biflavonoid (1) showed no antimicrobial activity at a concentration of 150 ${\mu}$g/disc. However, the crude extract of Quintinia acutifolia Kirk inhibited the growth of Bacillus subtilis and the dermatophytic fungus Trichophyton mentagrophytes. 2',3'-Dihydroochanaflavone (1) showed some cytotoxicity with IC$_{50}$ value of 3.1 ${\mu}$g/mL against P388 murine lymphocytic leukemia cells (positive control: mitomycin C IC$_{50}$ 0.06 ${\mu}$g/mL). The structure was determined by Spectroscopic methods.

• Journal of Digital Convergence
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• v.13 no.5
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• pp.337-344
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• 2015

Medicinal plants containing wellness-fusion-complex compound are increasingly being pursued as suitable alternative sources of various biological properties. In this study, inhibitory effect of Quintinia acutifolia, which is a New Zealand plant, on P388 murine lymphocytic leukemia cells using MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay. Based on $^1H-NMR$, $^{13}C-NMR$ spectral data and other spectral analysis, 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) inhibited the leukemia cells were purified from the plants. 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) are biflavonoids possessing two basic flavonoids and actively inhibited growth of P388 murine lymphocytic leukemia cells with a 50% inhibitory concentration ($IC_{50}$) of $8.2{\mu}g/mL$ and $3.1{\mu}g/mL$, respectively. Specially, 2'',3''-dihydroochana-flavone (3) possessed unconjugated flavonone system, which isn't consist of a pair with B ring of 2,3,2'',3''-tetrahydroochanaflavone (1). Therefore, the two compounds could be considered as a candidate for development of anticancer drugs and need to much studies in the future.

• Biomedical Science Letters
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• v.2 no.2
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• pp.175-185
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• 1996

The mitogen-activated protein(MAP) kinase signal transduction pathway represents an important mechanism by which mitogen, such as serum and PMA, regulate cell proliferation and differentiation. Target substrates of the MAP kinase are located within several compartments containing plasma membranes and nucleus. We now report that serum addition induces proliferation of the P388 murine leukemia cell, but PMA does not, while both serum and PMA treatment cause translocation of the MAP kinase, mainly p42$^{mapk}$ isoform, from cytosol into the nucleus, which was monitored by immunoblot analysis using polyclonal anti-ERK1 antibodies. We investigated whether the MAP kinase was capable of phosphorylating c-Jun protein and GST-fusion proteins, the P562$^{kk}$N-terminal peptides (1-77 or 1-123 domain) of the T cell tyrosine kinase, using the partially purified MAP kinase by SP-sephadex C-50, phenyl superose and Mono Q column chromatography. We found that the partially purified MAP kinase was able to phosphorylate c-Jun protein and the GST-fusion protein expressed using E.coli DH5$\alpha$ which is transformed with pGEX-3Xb plasmid vector carrying of p562$^{kk}$N-terminal peptide-encoding DNA. These results imply that tyrosine kinase receptor/Ras/Raf/MAP kinase pathway is a major mechanism for mitogen-induced cell proliferation in P388 murine leukemia cell and that the various MAP kinase isoforms may have their own target substrates located in distinct subcellular compartments.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.52-55
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• 1998

During a survey of new antitumor substances from higher plants, we have found that the crude extract of Simaba cedron Planchon (Simaroubaceae) showed cytotoxic activity (IC$\sub$50/ 0.7 $\mu\textrm{g}$/mL) against P388 leukemia cells. Activity-guided chromatographic purification using P388 cells led to the isolation of five novel quassinoids, cedronolactones A-E (1-5) and nine known quassinoids, simalikalactone D (6), chaparrinon (7), chaparrin (8), glaucarubolone (9), glaucarubol (10), samaderine Z (11), guanepolide (12), ailanquassin A (13), and polyandrol (14). In this seminar, the structural elucidation of 1-5 and the cytotoxic activity of the isolated compounds are discussed.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.13-15
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• 1994

Some known coumarins, decursin, nodakenetin, umbelliferone, 7-demethylsuberosin, columbianetin, decursinol angelate and decursinol, showing significant cytotoxic activities against P388 cell lines,were isolated from the roots of Angelica gigas (Umbelliferae) . 7-Demethylsuberosin and columbianetinwere obtained from Angelica gigas for the first time. Chernotaxonornic difference about coumarins com-ponents between the roots of Angelica gigas and those of A. acutiloba is also discussed.

• The Transactions of the Korean Institute of Power Electronics
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• v.17 no.5
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• pp.388-392
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• 2012

In this paper, a sampler model for the P-type current mode control employing low pass filter is proposed. Even though the frequency response of the compensator used in a P-type current mode control employing low pass filter is similar to that of P-type compensator, the sampler model has to be obtained from the method used in PI-type current mode control. In order to show the usefulness of the proposed method, prediction results of the proposed model are compared to those from the circuit level simulator, PSIM.

• Korean Journal of Pharmacognosy
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• v.31 no.1
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• pp.51-56
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• 2000

This study was carried out to evaluate cytotoxic effects of the roots of Sophora flavescens Ait. extracts on murine leukemia tumor cells lines $(P388D_1\;and\;L1210)$. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The comparison of $IC_{50}$ values of the ethyl acetate of Sophora flavescens Ait. extract in leukemia cell lines showed that their susceptibility to these extracts decreased in the following order : Adriamycin>Fr.4>Fr.5>Fr.3>Fr.1>Fr.2 by the MTT assay. These results suggest that the fraction 4 of the ethyl acetate soluble extract of Sophora flavescens Ait. may be a valuable choice for the studies on the treatment of murine leukemia cell lines.

• Natural Product Sciences
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• v.24 no.4
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• pp.284-287
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• 2018

A new isoprenylated acetophenone, acronyculatin P (1) as well as two known compounds, 3',5'-diisoprenyl-2',4'-dihydroxy-6'-methoxyphenylethanone (2) and 3'-isoprenyl-2',4',6'-trihydroxyphenylethanone (3) were isolated from the stem bark of Acronychia pedunculata (L.) Miq. The structures were determined by HRESIMS, 1D and 2D NMR. The inhibitory activity of the isoprenylated acetophenone derivatives against murine leukemia P-388 cells showed compound 1 moderate activity with $IC_{50}$ $15.42{\mu}M$.