• Title/Summary/Keyword: $L^1$-convergence

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Complete genome sequencing and comparative genomic analysis of Lactobacillus acidophilus C5 as a potential canine probiotics

  • Son, Seungwoo;Lee, Raham;Park, Seung-Moon;Lee, Sung Ho;Lee, Hak-Kyo;Kim, Yangseon;Shin, Donghyun
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1411-1422
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    • 2021
  • Lactobacillus acidophilus is a gram-positive, microaerophilic, and acidophilic bacterial species. L. acidophilus strains in the gastrointestinal tracts of humans and other animals have been profiled, but strains found in the canine gut have not been studied yet. Our study helps in understanding the genetic features of the L. acidophilus C5 strain found in the canine gut, determining its adaptive features evolved to survive in the canine gut environment, and in elucidating its probiotic functions. To examine the canine L. acidophilus C5 genome, we isolated the C5 strain from a Korean dog and sequenced it using PacBio SMRT sequencing technology. A comparative genomic approach was used to assess genetic relationships between C5 and six other strains and study the distinguishing features related to different hosts. We found that most genes in the C5 strain were related to carbohydrate transport and metabolism. The pan-genome of seven L. acidophilus strains contained 2,254 gene families, and the core genome contained 1,726 gene families. The phylogenetic tree of the core genes in the canine L. acidophilus C5 strain was very close to that of two strains (DSM20079 and NCFM) from humans. We identified 30 evolutionarily accelerated genes in the L. acidophilus C5 strain in the ratio of non-synonymous to synonymous substitutions (dN/dS) analysis. Five of these thirty genes were associated with carbohydrate transport and metabolism. This study provides insights into genetic features and adaptations of the L. acidophilus C5 strain to survive the canine intestinal environment. It also suggests that the evolution of the L. acidophilus genome is closely related to the host's evolutionary adaptation process.

Prevalence of chigger mites and Orientia tsutsugamushi strains in northern regions of Gangwon-do, Korea

  • Soojin Kim;In Yong Lee;Sezim Monoldorova;Jiro Kim;Jang Hoon Seo;Tai-Soon Yong;Bo Young Jeon
    • Parasites, Hosts and Diseases
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    • v.61 no.3
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    • pp.263-271
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    • 2023
  • The present study aimed to survey the prevalence of chigger mites and Orientia tsutsugamushi (O. tsutsugamushi) infection in the northern regions of Gangwon-do, Korea. From early February to early June 2015, a total of 17,050 chiggers were collected from striped field mice, Apodemus agrarius, in Cheorwon-gun, Hwacheon-gun, Yanggu-gun, and Goseong-gun, which are well-known endemic areas of scrub typhus in Korea. The chiggers were analyzed using molecular genomic methods, as previously described. Among the 7,964 identified chiggers, the predominant species was Leptotrombidium pallidum (76.9%), followed by L. zetum (16.4%), L. orientale (4.3%), L. palpale (0.3%), L. tectum (0.2%), and Neotrombicula tamiyai (1.8%). The chigger index (CI) was highest in Hwacheon (115.58), followed by Cheorwon (97.02), Yanggu (76.88), and Goseong (54.68). Out of the 79 O. tsutsugamushi-positive chigger pools, 67 (84.8%) were identified as the Boryong strain, 10 (12.7%) as the Youngworl strain, and only 2 were the Jecheon strain. Based on the high infestation of chiggers in striped field rodents and the high rate of O. tsutsugamushi infection in chigger mites, Hwacheon-gun and Cheorwon-gun are presumed to be high-risk areas for scrub typhus. Furthermore, L. pallidum, a major vector of scrub typhus, and the dominant O. tsutsugamushi serotype, the Boryong strain, were found in the northern regions of Gangwon-do, Korea.

ON THE LEBESGUE SPACE OF VECTOR MEASURES

  • Choi, Chang-Sun;Lee, Keun-Young
    • Bulletin of the Korean Mathematical Society
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    • v.48 no.4
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    • pp.779-789
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    • 2011
  • In this paper we study the Banach space $L^1$(G) of real valued measurable functions which are integrable with respect to a vector measure G in the sense of D. R. Lewis. First, we investigate conditions for a scalarly integrable function f which guarantee $f{\in}L^1$(G). Next, we give a sufficient condition for a sequence to converge in $L^1$(G). Moreover, for two vector measures F and G with values in the same Banach space, when F can be written as the integral of a function $f{\in}L^1$(G), we show that certain properties of G are inherited to F; for instance, relative compactness or convexity of the range of vector measure. Finally, we give some examples of $L^1$(G) related to the approximation property.

ON THE COMPLETE MOMENT CONVERGENCE OF MOVING AVERAGE PROCESSES GENERATED BY ρ*-MIXING SEQUENCES

  • Ko, Mi-Hwa;Kim, Tae-Sung;Ryu, Dae-Hee
    • Communications of the Korean Mathematical Society
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    • v.23 no.4
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    • pp.597-606
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    • 2008
  • Let {$Y_{ij}-{\infty}\;<\;i\;<\;{\infty}$} be a doubly infinite sequence of identically distributed and ${\rho}^*$-mixing random variables with zero means and finite variances and {$a_{ij}-{\infty}\;<\;i\;<\;{\infty}$} an absolutely summable sequence of real numbers. In this paper, we prove the complete moment convergence of {${\sum}^n_{k=1}\;{\sum}^{\infty}_{i=-{\infty}}\;a_{i+k}Y_i/n^{1/p}$; $n\;{\geq}\;1$} under some suitable conditions. We extend Theorem 1.1 of Li and Zhang [Y. X. Li and L. X. Zhang, Complete moment convergence of moving average processes under dependence assumptions, Statist. Probab. Lett. 70 (2004), 191.197.] to the ${\rho}^*$-mixing case.

A study on anti-inflammatory activity of fermented rice bran of Saccharomycopsis fibuligera isolated from Nuruk (누룩에서 분리한 Saccharomycopsis fibuligera 미강 발효물의 항염활성에 대한 연구)

  • Park, Yong-Won;Lee, Sang-Hyun
    • Journal of Convergence for Information Technology
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    • v.11 no.11
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    • pp.239-247
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    • 2021
  • This paper aims to increase functionality and competitiveness through fermentation of rice bran, which is used as a raw material for alternative foods and cosmetics. Cytotoxicity and anti-inflammatory effects were confirmed using rice bran extract fermented with Saccharomycopsis fibuligera A8 isolated from Nuruk. In the case of cytotoxicity, cytotoxicity was shown at 100 ㎍/mL for rice bran extracts, but cytotoxicity was not shown for fermented rice bran extracts. Meanwhile, as a result of confirming anti-inflammatory effects through inflammatory indicators such as nitric oxide, TNF-α, IL-1β, and IL-6, rice bran extracts showed anti-inflammatory effects at concentrations of 100 ㎍/mL or higher only nitric oxide and TNF-α. And fermented rice bran extracts exhibited anti-inflammatory effects at concentrations of 25 ㎍/mL, 25 ㎍/mL, 50 ㎍/mL, and 50 ㎍/mL, respectively, exhibiting anti-inflammatory effects in lower concentration. Meanwhile, as a result of testing the cytotoxicity and anti-inflammatory effects of heat-killed S. fibuligera, it was found that the heat-killed S. fibuligera showed anti-inflammatory effect in fermented products.

Desalinization Effect of Pennisetum Alopecuroides and Characteristics of Leachate Depending on Calcium Chloride (CaCl2) Concentration

  • Yang, Ji;Yoon, Yong-Han;Ju, Jin-Hee
    • Journal of People, Plants, and Environment
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    • v.23 no.4
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    • pp.445-453
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    • 2020
  • Background and objective: Calcium chloride (CaCl2) and sodium chloride (NaCl) are commonly used as a deicing agent in South Korea and penetrate the soil on the roadside, causing damage to plants. This study was conducted to investigate the salinity reduction effect of Pennisetum alopecuroides and the chemical characteristics of soil leachate. Methods: The plants were treated with five different concentrations of CaCl2 (0, 1, 2, 5, and 10g·L-1) and were grouped into the Cont., C1, C2, C5, and C10 groups. CaCl2 of 200 m·L-1 was sprayed to each plant once every two weeks. The growth of P. alopecuroides (plant height, leaf length, leaf width and the number of leaves) was measured. The level of EC and pH, and exchangeable cations (K+, Ca2+, Na+, and Mg2+) in the leachate of soil was monitored. Results: The pH of soil leachate decreased as the CaCl2 concentration increased, and the EC increased significantly. The content of K+ did not change significantly until the concentration of CaCl2 reached 5 g·L-1, but the content of Ca2+, Na+, and Mg2+ significantly increased. The plant height, leaf length, and leaf width of P. alopecuroides showed the highest value in CaCl2 1 g·L-1 followed by CaCl2 2 g·L-1 and the control group. Root fresh weight was the highest in CaCl2 2 g·L-1. On the other hand, there was no change in the shoot fresh weight, dry weight and root dry weight, and P. alopecuroides growth inhibition at the concentration of 5 g·L-1 or higher in the plant height and leaf length. Conclusion: P. alopecuroides is relatively highly salt-tolerant and can improve the salt damaged soil by lowering the content of the salt-based exchangeable K+ ions.

Defense Response and Suppression of Phytophthora Blight Disease of Pepper by Water Extract from Spent Mushroom Substrate of Lentinula edodes

  • Kang, Dae-Sun;Min, Kyong-Jin;Kwak, A-Min;Lee, Sang-Yeop;Kang, Hee-Wan
    • The Plant Pathology Journal
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    • v.33 no.3
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    • pp.264-275
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    • 2017
  • The spent mushroom substrate (SMS) of Lentinula edodes that was derived from sawdust bag cultivation was used as materials for controlling Phytophthora blight disease of pepper. Water extract from SMS (WESMS) of L. edodes inhibited mycelial growth of Phytophthora capsici, suppressed Phytophthora blight disease of pepper seedlings by 65% and promoted growth of the plant over 30%. In high performance liquid chromatography (HPLC) analysis, oxalic acid was detected as the main organic acid compound in WESMS and inhibited the fungal mycelium at a minimum concentration of 200 mg/l. In quantitative real-time PCR, the transcriptional expression of CaBPR1 (PR protein 1), CaBGLU (${\beta}$-1,3-glucanase), CaPR-4 (PR protein 4), and CaPR-10 (PR protein 10) were significantly enhanced on WESMS and DL-${\beta}$-aminobutyric acid (BABA) treated pepper leaves. In addition, the salicylic acid content was also increased 4 to 6 folds in the WESMS and BABA treated pepper leaves compared to water treated leaf sample. These findings suggest that WESMS of L. edodes suppress Phytophthora blight disease of pepper through multiple effects including antifungal activity, plant growth promotion, and defense gene induction.

ON SINGULAR INTEGRAL OPERATORS INVOLVING POWER NONLINEARITY

  • Almali, Sevgi Esen;Uysal, Gumrah;Mishra, Vishnu Narayan;Guller, Ozge Ozalp
    • Korean Journal of Mathematics
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    • v.25 no.4
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    • pp.483-494
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    • 2017
  • In the current manuscript, we investigate the pointwise convergence of the singular integral operators involving power nonlinearity given in the following form: $$T_{\lambda}(f;x)={\int_a^b}{\sum^n_{m=1}}f^m(t)K_{{\lambda},m}(x,t)dt,\;{\lambda}{\in}{\Lambda},\;x{\in}(a,b)$$, where ${\Lambda}$ is an index set consisting of the non-negative real numbers, and $n{\geq}1$ is a finite natural number, at ${\mu}$-generalized Lebesgue points of integrable function $f{\in}L_1(a,b)$. Here, $f^m$ denotes m-th power of the function f and (a, b) stands for arbitrary bounded interval in ${\mathbb{R}}$ or ${\mathbb{R}}$ itself. We also handled the indicated problem under the assumption $f{\in}L_1({\mathbb{R}})$.

Effect of Celeriac Extract on the LPS-Induced Production of Pro-inflammatory Cytokines by RAW 264.7 cells (셀러리악 추출물의 LPS로 유도된 전 염증성 사이토카인 생성에 미치는 영향)

  • Lee, Jae-Hyeok;Jeong, Hyun-Ju;Park, Jeong-Sook
    • Journal of the Korea Convergence Society
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    • v.12 no.2
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    • pp.295-300
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    • 2021
  • This study was conducted to investigate the effect of Celeriac Extract on the production of pro-inflammatory cytokines. Inflammation is caused by pro-inflammatory cytokines TNF-α, IL-6, IL-1β and mediators such as free radicals. We investigated the effect of Celeriac Extract (1ug/mL, 10ug/mL, 100ug/mL) on the production of proinflammatory cytokines such as TNF-α, IL-6, IL-1β and NO by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. These results demonstrated that Celeriac Extract inhibited the production of TNF-α, IL-6 and NO, without cytotoxicity significantly. Therefore, these findings suggest that Celeriac Extract may attenuate inflammatory responses via inhibition of production of pro-inflammatory cytokines and NO.

Proximate Analysis of Ipomea Batatass L. Grown in Two Different Zones in Imo State

  • meoka, N.U.;Ogbonnaya, C.I.;Ohazurike, N.C.
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.1
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    • pp.13-19
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    • 2019
  • Proximate analysis of Ipomea batatass L. grown in two different locations in Imo State were investigated. Standard soil analytical method was used to determine the physiochemical contents of the two soil sample collected from Mgbidi and Orji Ipomea batatass L. farm land. The soil sand from Ipomea batatass L. root in Orji farm recorded highest percentage value of 75.00% compared to the soil sand Ipomea batatass L. root in Mgbidi farm with 27.00% value. The percentage value of silt was different as the soil Ipomea batatass L. root in Mgbidi farm had high value of 29.40% while soil silt of Ipomea batatass L. root in Orji farm had 13.40%. The soil clay, pH, Phosphorus and Nitrogen from Ipomea batatass L. root in Mgbidi farm recorded highest percentage value of 43.60%, 5.7, 23.20 and 0.35 compared to the soil sand Ipomea root in Orji farm with 11.60%, 5.4, 16.70 and 0.09 value respectively. Ca, Mg, K, and Na analyzed followed the same trend as the soil from Ipomea root in Mgbidi farm had high percentage value of Ca (10.00), Mg (1.60), K (0.54) and Na (0.43) respectively. The systematic study of physiochemical of the Ipomea soils could help in understanding the nutritional composition, the basic characteristics of the soils and the constraints associated with the management of the soils from the two locations.