Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Celeriac Extract on the LPS-Induced Production of Pro-inflammatory Cytokines by RAW 264.7 cells

셀러리악 추출물의 LPS로 유도된 전 염증성 사이토카인 생성에 미치는 영향

  • Received : 2020.12.08
  • Accepted : 2021.02.20
  • Published : 2021.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the effect of Celeriac Extract on the production of pro-inflammatory cytokines. Inflammation is caused by pro-inflammatory cytokines TNF-α, IL-6, IL-1β and mediators such as free radicals. We investigated the effect of Celeriac Extract (1ug/mL, 10ug/mL, 100ug/mL) on the production of proinflammatory cytokines such as TNF-α, IL-6, IL-1β and NO by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. These results demonstrated that Celeriac Extract inhibited the production of TNF-α, IL-6 and NO, without cytotoxicity significantly. Therefore, these findings suggest that Celeriac Extract may attenuate inflammatory responses via inhibition of production of pro-inflammatory cytokines and NO.

본 연구는 Celeriac Extract의 전 염증성 사이토카인의 생성 억제에 미치는 영향을 살펴보고 억제 정도를 확인하기 위하여 실시되었다. 염증은 전 염증성 사이토카인인 TNF-α나 IL-6, IL-1β 및 활성산소와 같은 매개인자에 의해 나타난다. 이에 RAW264.7 세포에 lipopolysaccharide(LPS) 로 자극하여 생성된 TNF-α나 IL-6, IL-1β과 같은 전염증성 사이토카인과 NO 같은 활성산소에 대한 Celeriac Extract(1ug/mL, 10ug/mL, 100ug/mL)의 영향을 살펴보았다. 그 결과 Celeriac Extract은 세포 독성 없이 TNF-α나 IL-6의 생성과 NO생성을 유의성 있게 저해하였다. 따라서 이러한 결과는 셀러리악 추출물이 전 염증성 사이토카인 및 NO 생성을 억제하여 염증 반응을 약화시킬 수 있음을 시사한다.

Keywords

References

  1. B. Rocca & G. A. FitzGerald. (2002). Cyclooxygenases and prostaglandins shaping up the immune response. Int. Immunopharmacol. 2, 603-607. https://doi.org/10.1016/S1567-5769(01)00204-1
  2. C. Craing. (1994). Introduction to CNS Pharmacology. In Craing C. Stitzel R(eds.). Modern Pharmacology, 329-337.
  3. R. MacSween & K. Whaley. (1992). Muir's Textbook of Pathology, 13th ed. London: Edward Arnold,
  4. C. H. Kang-Rotondo, S. Major, T. M. Chiang, L. K. Myers & E. S. Kang. (1996). Upregulation of nitric oxide synthase in cultured human keratinocytes after ultraviolet B and bradykinin. Photodermatol Photoimmunol Photomed. 12, 57-62. https://doi.org/10.1111/j.1600-0781.1996.tb00176.x
  5. S. J. .Seo, H. G. .Choi, H, J. Chung & C. K. Hong (2002). Time course of expression of mRNA of inducible nitric oxide synthase and generation of nitric oxide by ultraviolet B in keratinocyte cell lines. Br J Dermatol. 147, 655-663. https://doi.org/10.1046/j.1365-2133.2002.04849.x
  6. R. L Shew , R. E. Papka , D. L. McNeill & J. A. Yee. (1993). NADPH-diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction. Peptides, 14, 637-642. https://doi.org/10.1016/0196-9781(93)90157-C
  7. H. Kwqamata, H. Ochiai , N. Mantani & K. Terasawa. (2000). Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells, a murine macrophage cell line. Am J Chin Med. 28, 217-226. https://doi.org/10.1142/S0192415X0000026X
  8. R. Weller (1997). Nitric oxide - a newly discovered chemical transmitter in human skin. Br J. Dermatol. 137, 665-671. https://doi.org/10.1046/j.1365-2133.1997.19332063.x
  9. M. Masaki M. Matsushita, & K. Wakitani. (1998). Inhibitory effect of JTE-522, a novel prostaglandin H synthase-2 inhibitor, on adjuvant-induced arthritis and bone changes in rats. Inflamm. Res. 47, 187-194. https://doi.org/10.1007/s000110050316
  10. W. Molly. (2014). All About Celery Root (Celeriac). localfoods.about.com.
  11. Growing Crops: Celery and Celeriac. (2011). Urban Organic.
  12. C. Hugh. (1911). Celery. Encyclopædia Britannica. Cambridge University Press.
  13. S. Wolfgang.(2012). Celeriac (Apium graveolens rapaceum). Desirable Vegetable Varieties, By Vegetable. The Owlcroft Company.
  14. W. L. Chen & Y. Xiao. (2020). Apigenin protects against ischemia-/hypoxia-induced myocardial injury by mediating pyroptosis and apoptosis. In Vitro Cellular & Developmental Biology, 13, 307-312.
  15. P. T. Ana, K. S. Slavina, S. B. Vasya, P. P. Milena, R. P. Dimitar, R. P. Plamen, L. S. Kiril, D. R. Mariya, K. S. Lachezar & V. V. Mila. (2015). The Effect of Apium Nodiflorum in Experimental Osteoporosis. Current Pharmaceutical Biotechnology, 16(5), 414-423. https://doi.org/10.2174/1389201015666141229104102
  16. A. Desai, T. Vyas & M. Amiji (2008). Cyroroxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J .Pharm Sci. 97, 2745-2750. https://doi.org/10.1002/jps.21182
  17. S. Wang, Y. Chen, D. He, L. He, Y. Yang, J. Chen & X. Wang. (2007). Inhibition of vascular smooth muscl cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation. J. Ethnopharmacol. 114, 458 https://doi.org/10.1016/j.jep.2007.08.039
  18. I. Iontcheva, S. Amar, K. H. Zawawi, A. Kantarci & T. E. Dyke.(2004). Role for moesin in lipopolysaccharide-stimulated signal transduction. Infect Immun. 72, 2312-2320. https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  19. N. Delanty & M. A. Dichter.(1998). Oxidative injury in the nervous system. Acta Neurol Scand. 98, 145-152. https://doi.org/10.1111/j.1600-0404.1998.tb07285.x
  20. A. Weisz, L. Ciatiello, & H. Esumi. (1996). Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine. Biochem. J. 316, 209 -214. https://doi.org/10.1042/bj3160209
  21. J. G. Qiu, L. Wang, W. J. Liu, J. F. Wang, E. J. Zhao, F. M. Zhou, X. B. Ji, L. H. Wang, Z. K. Xia, W. Wang, L. Z. Liu, Y. X. Huang & B. H. Jiang. (2019). Apigenin Inhibits IL-6 Transcription and Suppresses Esophageal Carcinogenesis Front. Pharmacol., 11 https://doi.org/10.3389/fphar.2019.01002