• 제목/요약/키워드: ${\alpha}$(X)

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Development of a Highly Active Fluorescence-Based Detector for Yeast G Protein-Coupled Receptor Ste2p

  • Hong, Jin Woo;Ahn, Hee Jun;Baek, Jee Su;Hong, Eun young;Jin, Dong Hoon;Khang, Yong Ho;Hong, Nam Joo
    • Journal of Microbiology and Biotechnology
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    • 제28권10호
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    • pp.1589-1603
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    • 2018
  • Twenty analogs of $[Orn^6,D-Ala^9]{\alpha}-factor$ were synthesized and assayed for their biological activities: seven analogs of $[Orn^6,X^9]{\alpha}-factor$, seven analogs of $[X^6,D-Ala^9]{\alpha}-factor$, five analogs of $[X^5,X^6,D-Ala^9]{\alpha}-factor$, and native ${\alpha}-factor$ (X = amino acids). Their biological activities (halo, gene induction, and affinity) were measured using S. cerevisiae Y7925 and LM102 and compared with those of native ${\alpha}-factor$ (100%). G protein-coupled receptor was expressed in strain LM102 containing pESC-LEU-STE2 vector. $[Dap^6,D-Ala^9]{\alpha}-factor$ with weak halo activity (10%) showed the highest receptor affinity (> 230%) and the highest gene induction activity (167%). $[Arg^6,D-Ala^9]{\alpha}-factor$ showed the highest halo activity (2,000%). The number of active binding sites per cell (about 20,000 for strain LM102) was determined using a newly-designed fluorescence-based detector, $[Arg^6,D-Ala^9]{\alpha}-factor-Edan$, with high sensitivity (12,500-fold higher than the absorption-based detector $[Orn^6]{\alpha}-factor-[Cys]_3$).

POSITIVE SOLUTION FOR SYSTEMS OF NONLINEAR SINGULAR BOUNDARY VALUE PROBLEMS ON TIME SCALES

  • Miao, Chunmei;Ji, Dehong;Zhao, Junfang;Ge, Weigao;Zhang, Jiani
    • 한국수학교육학회지시리즈B:순수및응용수학
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    • 제16권4호
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    • pp.327-344
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    • 2009
  • In this paper, we deal with the following system of nonlinear singular boundary value problems(BVPs) on time scale $\mathbb{T}$ $$\{{{{{{x^{\bigtriangleup\bigtriangleup}(t)+f(t,\;y(t))=0,\;t{\in}(a,\;b)_{\mathbb{T}},}\atop{y^{\bigtriangleup\bigtriangleup}(t)+g(t,\;x(t))=0,\;t{\in}(a,\;b)_{\mathbb{T}},}}\atop{\alpha_1x(a)-\beta_1x^{\bigtriangleup}(a)=\gamma_1x(\sigma(b))+\delta_1x^{\bigtriangleup}(\sigma(b))=0,}}\atop{\alpha_2y(a)-\beta_2y^{\bigtriangleup}(a)=\gamma_2y(\sigma(b))+\delta_2y^{\bigtriangleup}(\sigma(b))=0,}}$$ where $\alpha_i$, $\beta_i$, $\gamma_i\;{\geq}\;0$ and $\rho_i=\alpha_i\gamma_i(\sigma(b)-a)+\alpha_i\delta_i+\gamma_i\beta_i$ > 0(i = 1, 2), f(t, y) may be singular at t = a, y = 0, and g(t, x) may be singular at t = a. The arguments are based upon a fixed-point theorem for mappings that are decreasing with respect to a cone. We also obtain the analogous existence results for the related nonlinear systems $x^{\bigtriangledown\bigtriangledown}(t)$ + f(t, y(t)) = 0, $y^{\bigtriangledown\bigtriangledown}(t)$ + g(t, x(t)) = 0, $x^{\bigtriangleup\bigtriangledown}(t)$ + f(t, y(t)) = 0, $y^{\bigtriangleup\bigtriangledown}(t)$ + g(t, x(t)) = 0, and $x^{\bigtriangledown\bigtriangleup}(t)$ + f(t, y(t)) = 0, $y^{\bigtriangledown\bigtriangleup}(t)$ + g(t, x(t)) = 0 satisfying similar boundary conditions.

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OSCILLATION AND NONOSCILLATION THEOREMS FOR NONLINEAR DIFFERENTIAL EQUATIONS OF SECOND ORDER

  • Kim, Rak-Joong;Kim, Dong-Il
    • 대한수학회지
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    • 제44권6호
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    • pp.1453-1467
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    • 2007
  • By means of a Riccati transform some oscillation or nonoscillation criteria are established for nonlinear differential equations of second order $$(E_1)\;[p(t)|x#(t)|^{\alpha}sgn\;x#(t)]#+q(t)|x(\tau(t)|^{\alpha}sgn\;x(\tau(t))=0$$. $$(E_2),\;(E_3)\;and\;(E_4)\;where\;0<{\alpha}$$ and $${\tau}(t){\leq}t,\;{\tau}#(t)>0,\;{\tau}(t){\rightarrow}{\infty}\;as\;t{\rightarrow}{\infty}$$. In this paper we improve some previous results.

SEQUENTIAL INTERVAL ESTIMATION FOR THE EXPONENTIAL HAZARD RATE WHEN THE LOSS FUNCTION IS STRICTLY CONVEX

  • Jang, Yu Seon
    • Korean Journal of Mathematics
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    • 제21권4호
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    • pp.429-437
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    • 2013
  • Let $X_1$, $X_2$, ${\cdots}$, $X_n$ be independent and identically distributed random variables having common exponential density with unknown mean ${\mu}$. In the sequential confidence interval estimation for the exponential hazard rate ${\theta}=1/{\mu}$, when the loss function is strictly convex, the following stopping rule is proposed with the half length d of prescribed confidence interval $I_n$ for the parameter ${\theta}$; ${\tau}$ = smallest integer n such that $n{\geq}z^2_{{\alpha}/2}\hat{\theta}^2/d^2+2$, where $\hat{\theta}=(n-1)\bar{X}{_n}^{-1}/n$ is the minimum risk estimator for ${\theta}$ and $z_{{\alpha}/2}$ is defined by $P({\mid}Z{\mid}{\leq}{\alpha}/2)=1-{\alpha}({\alpha}{\in}(0,1))$ Z ~ N(0, 1). For the confidence intervals $I_n$ which is required to satisfy $P({\theta}{\in}I_n){\geq}1-{\alpha}$. These estimated intervals $I_{\tau}$ have the asymptotic consistency of the sequential procedure; $$\lim_{d{\rightarrow}0}P({\theta}{\in}I_{\tau})=1-{\alpha}$$, where ${\alpha}{\in}(0,1)$ is given.

$\alpha$-(Fe, Co)기 Nd-(Fe, Co)-B-Nb-Cu 초미세결정립합금의 자기특성에 미치는 Nd의 영향 (Influence of Nd Content on Magnetic Properties of Nanocrystalline $\alpha$-(Fe, Co)-Based Nd-(Fe, Co)-B-Nb-Cu Alloys)

  • 조덕호;조용수;김택기;송민석;김윤배
    • 한국자기학회지
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    • 제9권3호
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    • pp.154-158
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    • 1999
  • Nd 함유량을 2~6at.%로 변화시킨 $\alpha$-(Fe, Co)-B-Nb-Cu 초미세결정립합금의 미세조직 및 자기특성을 조사하였다. 급속응고된 Ndx(Fe0.9Co0.1)90-xB6Nb3Cu1(2$\leq$x$\leq$6, 1at.% 간격) 합금은 x=3 이상에서 비정질상으로 제조되었다. 제조된 비정질합금은 열처리에 의해 $\alpha$-(Fe, Co) 및 Nd2(Fe, Co)14B1상으로 구성된 초미세결정립합금으로 결정화 되었다. 최적열처리조건에서 잔류자화는 Nd의 증가에 따라 감소하며, x=3에서 1.55T로 최대 값을 나타내었다. 보자력은 Nd함유량 증가에 따라 직선적으로 증가하여 x=6에서 4.6kOe이었으며, 최대에너지적은 10.6MGOe였다.

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ON A FUZZY BANACH SPACE

  • Rhie, G.S.;Hwang, I.A.
    • 충청수학회지
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    • 제13권1호
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    • pp.71-78
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    • 2000
  • The main goal of this paper is to prove the following theorem ; Let (X, ${\rho}_1$) be a fuzzy normed linear space over K and (Y, ${\rho}_2$) be a fuzzy Banach space over K. If ${\chi}_{B_{{\parallel}{\cdot}{\parallel}}}{\supseteq}{\rho}*$, then (CF(X,Y), ${\rho}*$) is a fuzzy Banach space, where ${\rho}*(f)={\vee}{\lbrace}{\theta}{\wedge}\frac{1}{t({\theta},f)}\;{\mid}\;{\theta}{\in}(0,1){\rbrace}$, $f{\in}CF(X,Y)$, $B_{{\parallel}{\cdot}{\parallel}}$ is the closed unit ball on (CF(X, Y), ${\parallel}{\cdot}{\parallel}$ and ${\parallel}f{\parallel}={\vee}{\lbrace}P^2_{{\alpha}^-}(f(x))\;{\mid}\;P^1_{{\alpha}^-}(x)=1,\;x{\in}X{\rbrace}$, $f{\in}CF(X,Y)$, ${\alpha}{\in}(0,1)$.

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EXISTENCE AND CONCENTRATION RESULTS FOR KIRCHHOFF-TYPE SCHRÖ DINGER SYSTEMS WITH STEEP POTENTIAL WELL

  • Lu, Dengfeng
    • 대한수학회보
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    • 제52권2호
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    • pp.661-677
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    • 2015
  • In this paper, we consider the following Kirchhoff-type Schr$\ddot{o}$dinger system $$\{-\(a_1+b_1{\int}_{\mathbb{R^3}}{\mid}{\nabla}u{\mid}^2dx\){\Delta}u+{\gamma}V(x)u=\frac{2{\alpha}}{{\alpha}+{\beta}}{\mid}u{\mid}^{\alpha-2}u{\mid}v{\mid}^{\beta}\;in\;\mathbb{R}^3,\\-\(a_2+b_2{\int}_{\mathbb{R^3}}{\mid}{\nabla}v{\mid}^2dx\){\Delta}v+{\gamma}W(x)v=\frac{2{\beta}}{{\alpha}+{\beta}}{\mid}u{\mid}^{\alpha}{\mid}v{\mid}^{\beta-2}v\;in\;\mathbb{R}^3,\\u,v{\in}H^1(\mathbb{R}^3),$$ where $a_i$ and $b_i$ are positive constants for i = 1, 2, ${\gamma}$ > 0 is a parameter, V (x) and W(x) are nonnegative continuous potential functions. By applying the Nehari manifold method and the concentration-compactness principle, we obtain the existence and concentration of ground state solutions when the parameter ${\gamma}$ is sufficiently large.

Bacillus macerans에서 정제한 $\alpha$-cyclooextrin glucanotransferase의 구조와 칼슘이온이 기능에 미치는 영향 : X-ray 연구 (Structure and $Ca^{2+}$-ion effects on the function of $\alpha$-cyclodextrin Glucanotransferase from B. macerans : An X-ray study)

  • 최희욱;홍순강
    • KSBB Journal
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    • 제19권2호
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    • pp.159-163
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    • 2004
  • $\AA$의 분해능을 가진 Bacillus macerans에서 분리 정제한 cyclodextrin glucanotransferase의 결정 구조를 X-ray를 이용하여 분자 대치법을 사용하여 밝혔다. 정확한 구조는 16.6% ( $R_{free}$ = 20.5%)의 결정학적 R-factor를 가지고 있다. 두 개의 $Ca^{2+}$$_{-}$/ 이온이 점유된 새로운 금속 결합자리는 활성 자리의 도달 채널에서 발견되었다. $Ca^{2+}$$_{-}$/ 이온에 결합하는 음의 하전을 띤 아미노산 잔기들이 많이 밀집되어 있고 이 때, domain A ($\alpha$H에서 아미노산 잔기 283-297)의 중심부에 연결 부분은 $\beta$ 13-$\alpha$G 이었다. $\beta$13-$\alpha$G 부분은 활성 자리의 입구에 위치한 subsite 1에서 -1 과 Tyr260 (subsite 2)사이에 있는 촉매 부위 Glu258을 포함하고 있다. 비록 $\alpha$-CGTase 부류에서 잘 보존되어 있지는 않으나, 새로운 $Ca^{2+}$$_{-}$/ 자리 3a,b가 이 $\alpha$-CGTase의 활성도와 생성물의 특이성에 중요한 역할을 하리라 추측된다.다.

MULTIPLIERS OF WEIGHTED BLOCH SPACES AND BESOV SPACES

  • Yang, Gye Tak;Choi, Ki Seong
    • 충청수학회지
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    • 제22권4호
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    • pp.727-737
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    • 2009
  • Let M(X) be the space of all pointwise multipliers of Banach space X. We will show that, for each $\alpha>1$, $M(\mathfrak{B}_\alpha)=M(\mathfrak{B}_{\alpha,0})=H^\infty{(B)}$. We will also show that, for each $0<{\alpha}<1$, $M(\mathfrak{B}_\alpha)$ and $M(\mathfrak{B}_{\alpha,0})$ are Banach algebras. It is established that certain inclusion relationships exist between the weighted Bloch spaces and holomorphic Besov spaces.

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비정질 고체에 대한 X선의 다중 산란 강도 (The Intensity Scale of Multiple Scattering of X-rays in Non-Crystalline Solids)

  • 박성수;장윤식;류봉기;박희찬
    • 한국세라믹학회지
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    • 제34권1호
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    • pp.109-113
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    • 1997
  • 흡수계수가 매우 낮은 비정질 재료의 구조 해석을 하기 위하여 표준 X선 회절 기법을 이용했을 때, 시료의 측정된 X선 회절 강도에는 다중 산란에 의해 발생하는 X선 강도가 상당히 포함된다. 비정질 SiO2와 B2O3에서 사용되는 빔의 종류에 따라 발생하는 다중 산란 빔의 강도를 산출해 주는 컴퓨터 프로그램을 작성하였다. 여러 종류의 X선 빔과 시료 SiO2와 B2O3의 조합을 작성된 컴퓨터 프로그램을 도입하였을 때, 2$\theta$=0~180$^{\circ}$의 범위에서 시료 SiO2의 단일 산란 빔에 대한 다중 산란 빔의 강도 비는 CuK$\alpha$빔; 0.10~0.16%, MoK$\alpha$빔; 0.98~5.87%, AgK$\alpha$빔; 1.88~17.86%로 계산되어졌고, 시료 B2O3에 대한 단일 산란 빔에 대한 다중 산란 빔의 강도 비는 CuK$\alpha$빔; 0.27~0.54%, MoK$\alpha$빔; 2.30~19.69%, AgK$\alpha$빔; 3.96~53.83%로 계산되어졌다. 따라서, X선 회절 기법을 이용하여 비정질 SiO2와 B2O3의 구조 해석에 있어서는, MoK$\alpha$빔 및 AgK$\alpha$빔에서 측정된 X선 회절 강도는 다중 산란 효과에 대해서 반드시 교정하여야 한다.

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