Chronic asthma is considered as an incurable disease in modern society. This study focused on development of an animal model for the chronic asthma to investigate new drugs from traditional herbal medicine. And we tested the animal model with a typical prescription, Chungsangboha-tang and tried to find biochemical markers such as catecholamines and cAMP in serum, and as densities of beta-receptor in lung tissues. SD rats were actively sensitized by exposure to ovalbumine (OA). Ten days after sensitization, rats were challenged with OA aerosol by nebulizer six times every three days. Mucin was increased in bronchoalveolar lavages (BALs) after antigen (OA) challenge. Serum concentration of epinephrine was decreased significantly although there were not changed much in serum concentration of cAMP and the densities of beta-receptor in lung tissues. Chungsangboha-tang (5 g/kg/day) was given orally to ovalbumin-sensitized rats (n=8) for 15 days. Mucin in bronchoalveolar lavages (BALs) was increased significantly after treatment of Chungsangboha-tang although concentrations of epinephrine and cAMP were not changed significantly. The densities of beta-receptor in lung tissues were not different from those of controls. These results suggest that the ovalbumin-sensitized rats can be a good animal model of chronic asthma and Chungsangboha-tang is a possible drug in the treatment of chronic asthma.

To investigate recovery effects of Insambackhotang, which have been used clinically in diabetes therapny, on kidney failure of a diabetes-induced mouse by Alloxan administration, body and kidney weight changes of mice, BUN, creatinine, glucose and MDA level in serum, MDA level in kidney tissue. 1. A hyperglycemia(250-400mg/dl) mouse induced by Alloxan(75mg/kg) showed significant decline of kidney function: increase of BUN and creatinine in serum, excretion of glucose, protein, ketone in urine were observed at 4 days after the treatment. 2. Increase of the mouse body and kidney weight and a ratio of the kidney/body weight of Insambackhotang treated group as compared to the control group was significantly inhibited. 3. The BUN, creatinine level in serum of Insambackhotang treated group as compared to the control group were significantly inhibited. 4. The MDA level in serum and kidney tissue of Insambackhotang treated group as compared to the control group were significantly inhibited.

The goal of this study is to review and define the role of nurses' who are engaged in Korea Oriental medical treatments in oriental medical hospitals. We think this study can contribute to the development of 'Korea Oriental medical science & nursing science' and 'Public health care'. A large portion of nurses's role in Korea Oriental Medicine(KOM. 한방/한의학) is assistance to doctors treatment. But besides of these role, we think there are many things that are riskless for nurses to do alone. But in present situation, few nurses in KOM. have enough knowledge to treat these medical treatments alone. So we believe this study will provide a way for nurses to participate more actively in KOM. public health care. With the goal of this study, we checked all medical treatments that have been practiced in oriental medical hospitals, and classified these treatments with some groups. And we organized a inquiry. At this inquiry, we ask 'What is the adequate role of nurses in Korea Oriental medical treatment? & What kind of treatment can nurses do?' We got 58 responses from nationwide 121 hospitals. From these response, more than half of them said nurses can do following medical treatment in the oriental medical hospital: 1. starting and ending part of following treatments; External treatments by instrument, by hydrotheraphy, by herb, by suction, moxibustion, Manipulative therapies on soft structure, Living and mind-body therapies. 2. pulling out Acupuncture. 3. boiling herb, judgement on dosing temperature, assisting in dosing, 4. assisting Diet, 5. operating from Living and mind-body therapies. 6. leading Physical training However, these results are coming out from present situation. So, after well-oriented instructions for nurses, this study will be need to carry out again. From this study, we suggest a desirable curriculum for students who study 'Korea Oriental Nursing Science.' That is to say, at basic course students take 4 subject for 6 credits. And at as an expert course, it should be dividend into Clinical Nursing Specialist in KOM., Self-care Nursing Specialist, Regimen Nursing Specialist and take 17 credits per each course.

Vibrio are become prevailing if superficial temperature of ocean is raised and their activities of area are expanded and most of ocean creatures(fishes, oysters etc) are polluted with vibrio. The one who has taken these poluted fishes and aysters uncooked caused foodpoisoning and diarrhea from Vibrio. Frequencies of these diseases breakout is disposed in westsea shore of Korea. According to ancient and traditional Korean medical book -${\ulcorner}$Bangyak Happyeon${\lrcorner}$(Collection of Local Medicines, 1884) - and their single prescribes, we carried out experiment check the activities of natural medicinal effects on Vibrio parahemolyticus. The prescriptions of trial materials are processed from extraction boiling water and 80% methanol and followed freeze dried and adsorbed to every discs in dosage of 10mg. Gentamycin of 10mg were used for control. The result of compound prescription displayed special diseases in antimicrobial activities of boiling water and MeOH extraction compared with control. In compound prescription, extraction MeOH of Sashinhwan(clear zone : 17mm) presented extraordinaire antimicrobial activity. In single prescription, extraction of boiling water(clear zone : 16mm) and MeOH(clear zone : 18mm) of Fructus Chebulae presented extraordinaire antimicrobial activity. The MBC of Fructus Chebulae extracts was expressed in boiling water(1.28mg/ml) and MeOH(0.64mg/ml).

In this research we proceeded experiments to find the basis which make it possible to explain the physical and pathological process of Sasang constitutional medicine, in the way substituting hematopoietic-immune system(essence of life, blood, Ki and mental faculties : 精血氣神) Under these suppositions, the essence of life(精) is the multipotent stem cell which has the possibility to be specialized to any cell, the Ki(氣), blood(血) and mental faculties(神) are inferred that they are formed from specialized the essence of life(精), the blood(血) is the red blood cells and etc. that appears as the result of the genesis of circulation system. The Ki(氣) is from specialized basic immunity, the mental faculties(神) means long-term memories or combined immunity. Cytokines can act as specilaizing, growing factors and particiate in extremly combined procedure being controlled by both positive and negative specializing signals. Blood gathering was carried out in the morning and on empty stomach. The plasma was seperated and Erythropoietin, Stem cell factor, Granulocyte-colony stimulaing factor, Tumor necrosis factor, interlukin-3, Interleukin-6 were measured with ELISA kit. According to the result of post analysis by Duncan, each constitution is different in SCF(stem cell factor), IL-6(interleukin-6), EPO(erythropoietin). The value of Stem cell factor is high in order of Soumin(少陰人), Soyangin(少陽人), and Taeumin(太陰人), The value of interleukin-6 is high in Taeumin, Soumin, and Soyangin. Erythropoietin is high in order of Soumin, Soyangin, and Taeumin.

The result from the research on the disease classifications of the traditional medicine in China, Japan, Taiwan, and North Korea are followings: 1. It is remarkable that China has two different classifications. One is of the diseases named by western medicine and the other is of the syndromes compounded with parts, characters, and pathology of the diseases. The Traditional Chinese Medicine has 615 codes for diseases in 7 departments, and 1684 codes for syndromes. It seems that they have tried to match each disease named by the traditional chinese medicine to each one named by western medicine. But, they have left the diseases impossible to be equivalent to the ones in western medicine themselves and used the same codes of western medicine when the diseases are the same ones in western medicine. 2. In Taiwan, they try to connect the diseases named by the traditional medicine to the ones named by western medicine based on ICD-9. But, they did not attempt to classify the diseases of the traditional medicine by its own ways. The names of diseases in Taiwan medicine include both diseases and syndromes. It is limited to name syndromes by the traditional medicine. And, Taiwan medicine follows ICD in naming injuries. 3. Japan has not got the disease classification for the causes of death, but only the Japanese disease classification for the causes of death, a translation 'The international disease classification for the causes of death. Therefore, The diseases named by traditional medicines are excluded in the public medicine by some Japanese medicines which diagnose through the western medicine and treat by Wa Kang medicine. 4. I can't find out the data over the disease classification for the causes of death by traditional medicine in North Korea. Instead, I can refer to case histories in which differentiation of symptoms and signs and points about them by traditional medicine and the final diagnoses and report about examination by the western medicine has been recorded. In conclusion, It is a distinctive feature that they connect the diseases and the syndromes by the traditional medicine to the ones by the western medicine, and don't tell the diseases from the syndromes.

In Sasang constitutional medicine, the classification of Sasang constitutional types (Tae-Yang, Tae-Eum, So-Yang, and So-Eum) is important for the treatment. There are some problems with the methods used for classifying Sasang constitutional types; old methods such as pulse-palpation are not considered objective and recent methods such as immunohematology are considered expensive, painful and time-consuming. To overcome these problems, a body measurement and finger prints analysis were performed. The purpose of this study was to determine whether the palm prints and the palm creases could be helpful in classifying Sasang constitutional types. Thus, we looked for characteristics of the palm prints and the palm creases according to Sasang constitutional types. Before analyzing the palm prints and palm creases, 760 Korean (465 males, 295 females) were surveyed using two kinds of questionnaires for classifying Sasang constitutional types. As there were no Tae-Yang individuals, we were only able to identify the characteristics of the palm prints and the palm creases for Tae-Eum (288 persons), So-Yang (193 persons), and So-Eum (279 persons) individuals. In this study, the terminal points of D, closed crease, and open crease seemed to be helpful in classifying Tae-Eum and So-Eum individuals. Terminal point 11 and closed crease were frequent in Tae-Eum individuals; whereas, terminal point 7 and open crease were frequent in So-Eum individuals. Therefore, the palm prints and the palm creases seem to contribute to the classification of Sasang constitutional types.

세포열을 이용한 허혈/재관류 환경에서 청폐사간탕의 세포보호능을 측정하였다. 청폐사간탕 추출물은 허혈/재관류 환경하에서 발생하는 세포 독성으로부터 대표적 수용성 항산화제인 ascorbic acid보다 높은 세포 보호 활성을 나타내었으며, 산화적 손상의 지표로서 사용되는 지질과산화물(TBARS)를 측정한 결과에서도 ASA와 유사한 활성을 나타내었다. 또한, 허혈/재관류 환경하에서 활성이 증가하여 세포에 산화적 손상을 일으키는 활성 산소종을 유발하는 것으로 알려진 xanthine oxidase 활성 측정에서는 청폐사간탕이 ASA보도 높은 xanthine oxidase 활성 억제능을 보였으며, xanthine oxidase 효소 표품을 이용한 활성 억제능 측정에서도 ASA보다 뛰어난 결과를 보였다. 따라서, 청폐사간탕은 허혈/재관류 환경하에서 세포 보호능이 있는 것으로 추측이되며, 이러한 보호능은 항산화 활성과 더불어서 xanthine oxidase 활성 억제능이 공동 작용의 결과로 사료된다.

The effect of herbal medicine on 1-methyl-4-phenylpyridinium ion $(MPP^+)$ and 6-hydroxydopamine (6-OHDA) mediated neurotoxicity was studied in the rat phaeochromocytoma cell line PC12. The present study was designed to test the hypothesis that herbal medicine can protect cells from neurotoxiciy caused by $MPP^+$ and 6-OHDA. Exposure of PC12 cells to 0.2 mM $MPP^+$ and $50\;{\mu}M$ 6-OHDA for 24h resulted in a 50% cell death with respect to the control cells. $MPP^+$ induced cell death was reduced by Yollyounggobondan (延齡固本丹), Sagunjatang (四君子湯), Palmihwan (八味丸), and Palmultang (八物湯)(P<0.05). However, herbal medicines did not protect cells from degeneration caused by the 6-OHDA. Yollyounggobondan, Yungmijihwangwon (六味地黃元), Palmihwan, and Samultang (四物湯) were effective in protecting against $MPP^+$-induced ATP loss in PC12 cells (P<0.05). Yollyounggobondan and Palmultang were effect in neurite protection against 6-OHDA treatment in differentiated PC12 cells with NGF.

Korean Oriental Medicine has experienced a dramatic status change since the 19th century. Korean Oriental Medicine had been the only authentic medicine, but lost its status and fell as an illegal medicine through the Japanese rule, and recently revived successfully. The dramatic change of Korean Oriental Medicine needs an explanation. This paper explored the cause of the dramatic change and found the following things. First, the medical policy of the State directly influenced to the status of Korean Oriental Medicine. During the Japanese rule, the medical policy for Korean Oriental Medicine was very oppressive. After Liberation, a kind of noninterference policy was carried out. The change of the medical policy gave direct influence to the status of Korean Oriental Medicine. Second, the needs for medical service have been expanded. At the same time, the concern for tradition has been increased. And the supply of Korean Oriental Medicine has also increased by the establishments of Korean Oriental Medicine departments. Third, the internal growth of Korean Oriental Medicine brought to elevating the status of Korean Oriental Medicine. After 1945, Korean Oriental Medicine endeavored to strengthen the organization and to activate the academic society. Based on the above factors, Korean Oriental Medicine has achieved a remarkable status change during last several decades. We can not skip Korean Oriental Medical doctors' desperate efforts to enhance the status of Korean Oriental Medicine. Korean Oriental Medicine achieved the modernization. It's difficult to find similar case. It can be a model of modernization of tradition.