• 한국식품영양과학회지
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• 제34권9호
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• pp.1308-1313
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• 2005

구기자를 에탄올과 물로 추출하여 항산화와 항고혈압효과를 탐색하였다. 구기자 추출물의 폴리 페놀함량은 물 추출물과 에탄을 추출물에서 9.5 mg/g과 8.7 mg/g으로 나타났으며, HPLC에 의한 Polyphenol 화합물을 분리한 결과 물 추출물과 $60\%$ 에탄올에서 1.7 mg/g과 1.3 mg/g으로 chlorogenic acid가 가장 많이 검출되었다. 항산화효과 실험 중 DPPH는 물 추출물에서 $92.6\%$, 에탄을 추출물에서 $88.8\%$으로 높은 전자공여능을 나타냈으며, ABTS 측정에서는 물 추출물이 에탄을 추출물의 $52.8\%$보다 높은 $76.7\%$로 나타났다. PF는 물 추출물과 에탄을 추출물에서 각각 1.1과 1.0으로 나타났으며, TBARS값은 에탄을 추출물이 $1.5{\times}10^{-3}\;{\mu}M$ 로 대조구 $1.5{\times}10^{-3}\;{\mu}M$보다 낮은 TBARS 값을 나타내어 산화촉진인자를 binding하는 능력이 물 추출물보다 높게 나타났다. ACE 저해 활성은 물 추출물에서 $75.1\%$가, 에탄올을 추출물에서 $71.8\%$의 저해활성을 나타냈다. 이상의 결과로 구기자추출물이 기능성 식품소재로 활용이 가능할 것으로 사료된다.

• 한국식품영양과학회지
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• 제40권3호
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• pp.350-356
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• 2011

주름 미더덕(Styela plicata)을 8월부터 12월까지 매월 1회 채취하여 크기에 따라 큰 것(9.82~11.66 g)과 작은 것(0.93~2.21 g)으로 분류한 후 네 가지 용매(메탄올, 에탄올, 아세톤, 물)로 각각 추출 농축하여 추출물을 제조하였다. 각 추출물의 항산화 활성과 항고혈압 활성을 DPPH 라디칼 소거능과 환원력, 그리고 ACE 저해능으로 분석하였다. DPPH 라디칼 소거능은 11월에 채취된 큰 주름 미더덕의 아세톤 추출물이 가장 높았고, 환원력은 11월의 작은 미더덕의 아세톤 추출물이 가장 높았다. ACE 저해능은 물 추출물로만 측정하였는데, 11월에 채취된 큰 주름 미더덕의 추출물이 가장 높았다. 또한 주름 미더덕은 채취시기, 크기, 추출용매에 상관없이 거의 모든 시료에서 $H_2O_2$에 의해 유도된 백혈구의 DNA 손상을 억제하는 것으로 나타났으며 그 효과는 특히 8월 또는 9월에 채취한 큰 주름 미더덕이 월등함을 알 수 있었다. 또한 DNA 손상 억제에 있어서 추출 용매별 차이는 미미한 것으로 나타났으며 오히려 일부 물 추출물은 다른 용매 추출물에 비해 그 효과가 큰 것으로 나타나 끓이기 등의 일상 조리를 통한 주름 미더덕의 유효 성분 섭취를 기대할 수 있겠다. 이상의 결과로 채취시기, 크기, 용매에 따라 항산화, 항유전 독성 및 ACE 저해능의 차이가 있으며, 이는 주름 미더덕을 기능성 소재로 활용할 때 기초 자료로 활용될 수 있을 것이다.

• 한국식품영양과학회지
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• 제33권2호
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• pp.255-261
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• 2004

뽕나무가루 첨가 배지에서 배양한 느타리버섯균사체 배양물의 조추출물이 자유라다칼로 유도한 linloeic acid의 산화를 대조구에 비해 75.9% 감소시켰고, mouse liver microsome산화에서도 NADPH/Fe$^{++}$ system에서 64.3% 감소시켰다. 이와 같은 효과는 조추출물로부터 용매분획한 분획물의 단독 효과보다 우수하였다. 상황 및 동충하초버섯균사체 배양물의 조추출물도 느타리 버섯균사체 배양물의 조추출물과 효과가 유사하였지만 다소 낮았다.

• 한국수산과학회지
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• 제56권6호
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• pp.833-840
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• 2023

In this study, the antioxidant and anti-skin aging properties of the Korean marine algae Ishige foliacea were investigated. Solvent extracts from I. foliacea were prepared with 70% ethanol, 80% methanol, and water. The extraction yields of various solvent extracts ranged from 9.55% to 35.12%. In terms of antioxidant activity, the ethanol extract showed the highest ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activity, nitrite oxide scavenging activity, reducing power, and FRAP (ferric reducing antioxidant power). Regarding anti-skin aging activity, evaluation of the skin whitening and anti-wrinkle activities revealed that the methanol, water, and ethanol extracts possessed the highest tyrosinase (IC₅₀=0.98 mg/mL), elastase (IC₅₀=0.15 mg/mL), and collagenase (IC₅₀=0.06 mg/mL) inhibitory activities, respectively. These results suggest that I. foliacea holds potential as an antioxidant and anti-skin aging substance in food and cosmetic materials.

• 한국수산과학회지
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• 제42권3호
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• pp.197-203
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• 2009

This study was conducted to investigate the food components of cooking drips from shell oysters steamed under various retort pressures. Among the drips from shell oyster steamed under different retort pressures (1.0, 2.0, 2.5, 3.0, 3.5, 4.0 and 4.5 kg/$cm^2$) the oyster drip obtained at the 4.5 kg/$cm^2$ of retort pressure had the highest degree of brix, yield, crude protein, glycogen and trichloroacetic acid soluble-N contents, while its salinity was the lowest. In the results of food safety test, the presence of E. coli (18 MPN/100 g>) and viable cell (30 CFU/g>) in the oyster drip was in acceptable level as a food-stuff. However, the sensory evaluation such as color, flavor and taste, total amino acid and free amino acid contents of cooking drip from shell oyster steamed at 4.5 kg/$cm^2$ were inferior to those of oyster wash water. Differences in the major amino acids of total amino acid and free amino acid between oyster cooking drip and oyster wash water were also found. The results suggested that the effective use methods of oyster cooking drip should be investigated.

• 산업식품공학
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• 제15권4호
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• pp.305-310
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• 2011

본 연구에서는 DSM을 이용하여 단백질 추출이나 정제없이 식품에 적용 가능성이 있는 가식성 필름을 개발하였다. HPH 처리는 표면이 매끄럽고 균일한 형태의 필름을 형성시켰고, 필름의 WVP값을 감소시켜 수분 방벽 효과를 높였다. 필름 내 글리세롤 농도 증가는 WVP값을 전반적으로 높였다. 농산물 가공 부산물인 DSM으로 제작된 가식성 필름은 새로운 식품 소재로서 상업적 적용 가능성을 보여주었다.

• 한국축산식품학회지
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• 제41권6호
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• pp.1049-1059
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• 2021

The objective of this study was to evaluate the nutritional content of bullfrog meat from different parts of the animal, including fore-chest, thigh and calf. Bullfrog meat was found to be a rich source of proteins, essential amino acids and minerals, but with a low fat content, compared with other aquatic meat products. There was no significant difference (p>0.05) between thigh and calf in mineral content (K, P, Na, Mg, Ca, Zn, Fe, Cu, and Mn), but the contents of K, P, and Mg were higher in thigh and calf than in the fore-chest (p<0.05). The salt-soluble, water-soluble and insoluble protein bands in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, from fore-chest, thigh and calf were similar, with the most abundant bands being 35 kDa (salt-soluble protein), 35-48 kDa (water-soluble protein) and 48 kDa (insoluble protein). The results showed that the insoluble protein content in the fore-chest meat was higher than that in the thigh meat and calf meat, but the salt-soluble protein fraction was the most abundant in thigh meat. These results showed that the nutrients in different parts of bullfrog meat were different.

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.1976-1980
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• 2010

A simple and reliable method has been developed to selectively separate and concentrate trace amounts of Fe(III) ions from water and food samples by using flame atomic absorption spectrometry. A new reagent, 5-hydroxy-4-ethyl-5,6-di-pyridin-2-yl-4,5-dihydro-2H-[1,2,4] triazine-3-thione, was synthesized and characterized by using FT-IR spectroscopy and elemental analysis. Effects of pH, concentration and volume of elution solution, sample flow rate, sample volume and interfering ions on the recovery of Fe(III) were investigated. The optimum pH was found to be 5. Eluent for quantitative elution was 10 mL of 2 M HCl. The preconcentration factor of the method, detection limit (3s/b, ${\mu}gL^{-1}$) and relative standard deviation values were found to be 25, 4.59 and 1%, respectively. In order to verify the accuracy of the method, two certified reference materials (TMDA 54.4 lake water and SRM 1568a rice flour) were analyzed. The results obtained were in good agreement with the certified values. The method was successfully applied to the determination of Fe(III) ions in water and food samples.

• Food Science and Biotechnology
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• 제14권2호
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• pp.190-195
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• 2005

The conditions for the extraction of effective substances from the stem of Opuntia ficus-indica were optimized by a response surface methodology. The total extract yield was maximized under the temperature of $97.77^{\circ}C$, at a time duration of 145.82 min and a water to sample ratio 16.59 mL/g. Moreover, the optimum conditions for the extraction of effective substances were as follows: $84.95^{\circ}C$, 156.50 min and a water/sample ratio of 7.46 mL/g for the phenolics content; and $97.11^{\circ}C$, 139.03 min and a water/sample ratio of 10.91 mL/g for the pectin content. The range of optimum extraction conditions in consideration of the physicochemical properties of the extracts were shown to be as $95-100^{\circ}C$ as the extraction temperature, 120-180 min as extraction time and a water to sample ratio of 5-18 mL/g.

• 한국수산과학회지
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• 제6권3_4호
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• pp.97-100
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• 1973

In the previous work(Kim et al, 1973), the quality of sun-dried Alaska pollack, Theragra chalch-ogramma, was discussed in the aspect of non-enzymatic discoloration as a function of relative humidity during storage at room temperature($20^{\circ}C$). In this paper, sun-dried squid, Ommastrephes steam pacificus was investigated at the same aspect mentioned above. Fresh squid from the whole sale market was dressed, filleted, dried for 48 hours in the open air and finally stored in the humidistat chamber. Lipid oxidation ana development of non-enzymatic browning were tested by the same methods described in the previous paper. The TBA value showed a maximum peak on 30 day storage, hereafter tended to decrease gradually. The rate of browning, however, in water soluble fraction as well as in chloroform-methanol fraction was lower at 0.34 to 0.45 water activity than at any other case, and propagation of lipid oxidation was also diminished at the above level of water activity. From the results, it is recognized that storage at Aw=0.34 to 0.45 provides higher quality stability for sun-dried squid.