• 치위생과학회지
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• 제15권2호
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• pp.238-245
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• 2015

본 연구는 지각과민처치제에 항균 물질을 첨가하여 항균실험 및 지각과민처치제의 세포독성실험을 실시하여 다음과 같은 결과를 얻었다. 항균실험에서는 Micro Prime은 항균 약물의 농도가 높을수록 항균력도 높게 나타나 각 그룹간의 항균 범위는 통계적인 유의차를 나타내었다(p<0.05). Hurri Seal에서는 gallic acid 항균 약물 농도가 높을수록 항균력이 높게 나타났지만(p<0.05), tetracycline을 함유한 desensitizer에서는 농도가 높을수록 항균효과는 떨어지는 것으로 나타났다(p<0.05). 또한 chlorhexidine과 cetylpyridinium chloride에서는 대조군에서만 차이를 나타내고(p<0.05), 각 실험군에서 차이를 나타내지 않았다. 세포독성도를 평가한 결과 Micro Prime은 미약한 세포독성을 나타냈으며, Hurri Sea은 심한 세포독성을 나타냈다. 이상의 연구결과로 지각과민처치제에 항균물질을 첨가할 경우 우수한 항균력이 나타났기 때문에 지각과민 환자의 동통을 줄여 줄 것으로 사료되며 항균성을 가진 지각과민처치제로 사용이 가능할 것으로 판단된다.

• 동의생리병리학회지
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• 제17권1호
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• pp.123-129
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• 2003

Experimental studies were done to research the dinical effects of Juglandis Semen aqua-acupuncture and acupuncture(BL 13) on the anti-allergic response. anaphylaxis provoked by the compound 48/80, delayed type hypersensitivity response to picryl chloride and SASC and inflammation response to egg albumin. The following results have been obtained: Juglandis Semen aqua-acupuncture and acupuncture(BL13) group were increased the survival rate in compound48/80 induced systemic anaphylactic reaction. Picryl chloride induced contact dermatitis and delayed type hypersensitivity in SRBC challenged mouse were significantly decreased in Juglandis Semen aqua-acupuncture and acupuncture (BL13) group. Inflammation response - WBC, CRP and Nitric Oxide in egg albumin induced allergic rat were significantly decreased in Juglandis Semen aqua-acupuncture and acupuncture(BL13) group. According to the above results, Semen aqua-acupuncture and acupuncture(BL13) both depress the allergy reaction.

• Environmental Analysis Health and Toxicology
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• 제2권1_2호
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• pp.33-42
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• 1987

Experiments were performed to investigate effects of ethanol and saccharin on the immune system in rats. 4% ethanol and 0.02, 0.20, 2.00% saccharin solution in 4% ethanol were provided ad libitum by tap water for 4 weeks. Rats were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by relative immuno organ weight, antibody production, Arthus reaction, delayed type hypersensitivity, and rosette forming cell. Ethanol exposure decreased thymus weight and delayed type hypersensitivity. A combined solution of ethanol and saccharin decreased water intake, growth rate, spleen weight, thymus weight, humoral and cellular immune response. Especially, a 2% saccharin solution in 4% ethanol very significantly suppressed cellular immunity.

• 생약학회지
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• 제14권3호
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• pp.102-106
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• 1983

As many crude drugs are used in the oriental medical field problems on the side effects of these drugs come to the front. To conduct delayed-type hypersensitivity we selected 29 kinds of drugs used frequently for therapeutic agents in oriental medical hospitals (Table I). The cell-mediated immune response was evaluated by measuring the foot pad swelling reaction and humoral immune response by measuring the antibody formation to these crude drugs. Mice were given these drugs intraperioneally for sensitization and challenged with same drug as used for sensitization respectively by intral dermal injection on the left and righ hind foot pad 4 days after senstization and then the foot pads were measured with the dial micrometer. The results were as follow; 1) Gentianae Scabrae Radix, Arecae Semen, Corydalis Tuber, and Paeoniae Radix were significant as delayed-type hypersensitivity inducers. 2) None of the crude drugs tested had effect on the induction of humoral immune response.

• 약학회지
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• 제46권1호
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• pp.52-57
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• 2002

Effects of the butanl fraction of Astragali Radix (BFAR) on the cellular and nonspecific immune responses were investigated in ICR mice. Mice were divided into 4 groups and BFAR at doses of 5, 25 and 125 mg/kg u ere administered orally to mice daily for 3 weeks, and the normal animals were given vehicle. The results of this study are summarized as follows; the relative weight of thymus was greatly increased by BFAR treatment, compared with that in mormal mice. However, the body weight gain was not affected. Delayed-type hypersensitivity (DTH) reaction to sheep red blood cells (SRBC) for cellular immunity was significantly enhanced by BFAR treatment, compared with those in normal mice. In these mice, BFAR also dose-dependently increased activities of phagocyte and natural killer (NK) cells as well as the number of leukocyte resulted from nonspecific immunity Thus, these results demonstrate that BFAF treatment results in a significant increase in both cellular and nonspecific immune responses to antigen in concentrations that enhance humoral immune function.

• Archives of Pharmacal Research
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• 제26권4호
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• pp.312-316
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• 2003

The oriental herbal combination allergina has been shown to inhibit allergic inflammation. In the present study, we demonstrate that the oral administration of allergina markedly inhibits the progression of inflammatory diseases, such as graft-versus-host diseases (in the allogeneic bone marrow transplantation and the parent-into-F1 transplantation models), collagen-induced arthritis and sheep red blood cell-induced delayed type hypersensitivity. The immunosuppressive activity of allergina in vivo appears to be associated, at least in part, with the inhibition of tumor necrosis factor-a production. In conclusion, our results suggest that allergina could be useful as a immunosuppressive agent for the treatment of macrophage-related inflammatory disease.

• 대한미생물학회지
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• 제21권1호
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• pp.145-149
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• 1986

The development of delayed-type hypersensitivity(DTH) reaction to Staphylococcus aureus in mice was studied, Mice received 3 injections of $10^8$ viable S. aureus subcutaneously showed a marked footpad swelling when mice were challenged with $10\;{\mu}g$ staphylococcal protein antigen into footpad(The percent increase of footpad thickness at 24 h after challenge wsa 35% approximately). Histological observation of footpad of immunized mice showed a marked thickness of subcutaneous tissue due to edematous reaction and massive infiltration of lymphocytes and neutrophils which are characteristic cells in DTH reaction. Intensity of DTH reaction of mice immunized with viable bacteria was much higher than that of mice immunized with staphylococcal protein or heat-killed bacteria. The DTH reaction to S. aureus could be transferred to normal recipient mice by both spleen cells and lymph node cells.

• 동의생리병리학회지
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• 제19권6호
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• pp.1604-1609
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• 2005

The purpose of this research was to investigate the effects of Bopaewon-tang (BT) on allergic reaction. In the present study, we examined the effect of BT on type 1 and type tV allergic reaction. BT (500 mg/kg) did not affect the systemic anaphylaxis induced by compound 48180 and the passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin in vivo. Also, BT did not affect the release of histamine from peritoneal mast cells in rats. In addition, BT did not affect the permeability of evans blue into peritoneal cavity, but inhibited the writhing syndrome induced by acetic acid. BT inhibited the delayed type hypersensitivity induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene. These results indicate that BT may be useful for the prevention and treatment of type IV allergy related disease.

• 대한한방소아과학회지
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• 제8권1호
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• pp.27-37
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• 1994

This Experimental study was done to investigate the effect of the Taklisodok-um on the Anti-allergic response. The results were obtained as follows: 1. On vascular permeability response to the intradermal injected Histamine, the Taklisodok-um treated group revealed more significant decrease than control group. 2. On vascular permeability response to the intradermal injected Serotonin, the Taklisodok-um treated group revealed more significant decrease than control group. 3. In the 48hrs homologous passive cutaneous anaphylaxis provoked by the IgE-like antibody against white egg albumin, there was no significant difference between the Taklisodok-um treated group and control group. 4. The Taklisodok-um treated group revealed more significant inhibitory effect than control group in the delayed type hypersensitivity response to Picryl chloride. 5. The Taklisodok-um treated group revealed more significant inhibitory effect than control group in the delayed type hypersensitivity response to SRBC. According to the above-stated results, it is considered that the Taklisodok-um could be applied widely to the type I and IV allergic diseases.

• Korean Journal of Acupuncture
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• 제20권4호
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• pp.53-63
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• 2003

Objective : Experimental studies were done to research the clinical effects of Herba Patriniae(HP) aqua-acupuncture ($BL_{13},\;BL_{17},\;BL_{13}{\cdot}BL_{17}$ and free points) on the anti-allergic inflammatory response. Methods : We measured active systemic anaphylatic shock induced by compound 48/80 and microvascular permeability increased by acetic acid. And we measured total IgE and plasma WBC level, serum total protein, albumin, immunoglobulin and NO levels induced by egg albumin. Results : HP aqua-acupuncture pretreatments at all acupoints inhibited active systemic anaphylatic shock induced by compound 48/80 and microvascular permeability increased by acetic acid. Total IgE and plasma WBC level inhibited by HP aqua-acupuncture pretreatment at $BL_{13}\;BL_{17}$ and free points. However, HP aqua-acupuncture didn't effect serum total protein, albumin, immunoglobulin and NO levels. Conclusion : These results suggest that HP aqua-acupuncture may be beneficial in the regulation of type Ⅰ allergic reaction, but is further required immunological studies on the allergic reaction.