The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Induction of Delayed-Type Hypersensitivity Reaction to Staphylococus aureus in Mice

황색포도상구균에 대한 마우스의 지연성과민반응 발현

  • Lee, Hern-Ku (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
  • Choi, Tai-Hoon (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
  • Ha, Tai-You (Department of Microbiology and Immunology, Chonbuk National University Medical School)
  • 이헌구 (전북대학교 의과대학 미생물학교실) ;
  • 최태훈 (전북대학교 의과대학 미생물학교실) ;
  • 하대유 (전북대학교 의과대학 미생물학교실)
  • Published : 1986.03.31
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Abstract

The development of delayed-type hypersensitivity(DTH) reaction to Staphylococcus aureus in mice was studied, Mice received 3 injections of $10^8$ viable S. aureus subcutaneously showed a marked footpad swelling when mice were challenged with $10\;{\mu}g$ staphylococcal protein antigen into footpad(The percent increase of footpad thickness at 24 h after challenge wsa 35% approximately). Histological observation of footpad of immunized mice showed a marked thickness of subcutaneous tissue due to edematous reaction and massive infiltration of lymphocytes and neutrophils which are characteristic cells in DTH reaction. Intensity of DTH reaction of mice immunized with viable bacteria was much higher than that of mice immunized with staphylococcal protein or heat-killed bacteria. The DTH reaction to S. aureus could be transferred to normal recipient mice by both spleen cells and lymph node cells.

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