• Title/Summary/Keyword: total phenols

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Evaluation of Baby-leaf Growth and Leaf Red Color Intensity for Amaranthus Germplasm (아마란스(Amaranthus ssp.) 유전자원의 어린잎 생육과 엽 적색도 평가)

  • Lee, Jun-Gu;Jang, Yoon-Ah;Um, Yeong-Cheol;Lee, Sang-Gyu
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.59-65
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    • 2012
  • This study evaluated the baby-leaf growth, leaf red color intensity, and phytochemical compounds, such as total phenols and total flavonoids using domestic Amaranthus accessions, to select promising germplasm for the use of baby-leaf vegetable. The 39 accessions, upper 15% of 262 Amaranthus germplasm collection showing high red color intensity in baby-leaf, measured as Hunter's a value, were primarily selected. The eleven accessions including IT199970, showing stable red color intensity in baby-leaf both in high-temperature and low temperature cultivation, were finally selected as promising germplasm for the use of baby-leaf vegetable Amaranthus. The leaf red color intensities when they were cultivated at low temperature period were generally higher than those at low temperature period, and all the eleven selected germplasm, including four Amaranthus sub-species and five unknown species, showed stable red color expression in baby-leaf. The total phenol and total flavonoid contents were also presented for finally selected germplasm including their baby-leaf fresh weights. These finally selected germplasm could be potentially used as baby-leaf vegetable resources, through fortifying the color balance of salad vegetable.

Phenol Contents of Solvent Extraction in Several Domestic Thymus Quinquecostatus Celak (국내 자생 백리향의 추출용매에 따른 페놀함량)

  • Baik, Jung-Ae;Baek, Yi-Hwa;Chiang, Mae-Hee
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.468-474
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    • 2009
  • Thyme leaves of aerial part in 5 domestic species was extracted by several solvens, and chemical composition of extracts was analyzed by GC-MS to determine the effective solvent for phenol compound extracts. Total phenol contents containing thymol and carvacrol according to methanol extracts of Ulleung island, Jeju middle mountain, Kyeonggido, Jeju alpine species, and Gangwondo were 92.21, 84.92, 77.43, 74.16, and 73.51%, while them of ethanol extracts of Gangwondo, Jeju middle mountain, Kyeonggido, Ulleung island, and Jeju alpine species were in the order of and their contents were 93.64, 93.62, 90.74, 89.52, and 72.65%, separately. Hexane extracts of Gangwondo and Ulleung island thyme were almost composed by phenol contents containing thymol for 100 and 95.71%.

Quality Characteristics and Antioxidant Activities of Peach Makphyun (복숭아 막편의 품질 특성 및 항산화성)

  • Shim, Eun Kyoung;Kim, Hyun Jeong;Kim, Mee Ree
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.11
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    • pp.1724-1730
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    • 2014
  • The objective of this study was to develop peach Makphyun, a kind of rice cake, added with peach and Makgeolli. The effects of peach paste (0, 5, 10, or 20%) on quality characteristics of Makphyun were evaluated during storage at $20{\pm}2^{\circ}C$ for 3 days. As the concentration of peach paste increased, pH decreased and acidity increased. Reducing sugar contents (%) increased with the amount of peach paste. Hunter color b (yellowness) value of rice cake increased with increasing amount of peach paste, whereas L (lightness) and a (redness) values decreased. Contents of total phenols increased with increasing amount of peach paste. Antioxidant activities such as DPPH and hydroxyl radical scavenging activities of peach Makphyun decreased with increasing amount of peach paste. The sensory evaluation results showed that overall preference of Makphyun containing peach paste content of 20% is higher than other groups.

An In Vitro and In Vivo Cholinesterase Inhibitory Activity of Pistacia khinjuk and Allium sativum Essential Oils

  • Ghajarbeygi, Peyman;Hajhoseini, Ashraf;Hosseini, Motahare-Sadat;Sharifan, Anoosheh
    • Journal of Pharmacopuncture
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    • v.22 no.4
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    • pp.231-238
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    • 2019
  • Objectives: Alzheimer's disease (AD), an overwhelming neurodegenerative disease, has deleterious effects on the brain that consequently causes memory loss and language impairment. This study was intended to investigate the neuroprotective activity of the two essential oils (EOs) from Iranian Pistacia khinjuk (PK) leaves and Allium sativum (AS) cloves against β-Amyloid 25-35 (Aβ25-35) induced elevation of cholinesterase enzymes in AD. Methods: The EOs of PK (PKEO) and AS (ASEO) were prepared and analyzed in terms of extraction yield, phenolic content, and cholinergic markers in vitro. Moreover, both were administered orally to adult male Wistar rats at concentrations of 1, 2, and 3%. The inhibitory potential of PKEO and ASEO was compared with Donepezil (0.75 mg/kg) against the high activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Results: PKEO reached an inhibition rate of 83.6% and 81.4% against AChE and BChE, respectively. ASEO had lower anti-cholinesterase activity (65.4% and 31.5% for the inhibition AChE and BChE). PKEO was found to have more phenolic content than ASEO. A significantly positive correlation was observed between the total phenolics and anti-cholinesterase potential. In rats, both EOs decreased the enzyme activity in a concentration-dependent manner. As compared with Donepezil, the significant difference in the AChE and BChE inhibition occurred as rats were treated with PKEO 3% (p < 0.05). Conclusion: It could be concluded that PKEO and ASEO are potent inhibitors of AChE and BChE in rats that hold promise to be used for the treatment of AD.

Lipid-lowering and hepatoprotective effects of Vitis vinifera dried seeds on paracetamol-induced hepatotoxicity in rats

  • Almajwal, Ali Madi;Elsadek, Mohamed Farouk
    • Nutrition Research and Practice
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    • v.9 no.1
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    • pp.37-42
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    • 2015
  • BACKGROUND/OBJECTIVES: Red grape seeds as functional food are a good source of important bioactive components such as phenolics and antioxidants, which decrease oxidative stress that contributes to the pathogenesis of hepatotoxicity. The current study was conducted in order to evaluate the protective effect of red grape dried seeds (RGDS) on antioxidant properties, lipid metabolism, and liver and kidney functions of rats with paracetamol (750 mg/kg) induced hepatotoxicity. MATERIALS/METHODS: RGDS was added to the basal diet at 5, 10, and 20%. Thirty five adult male rats were assigned to five groups (n = 7) for a six-week feeding period; group (1) normal control, group (2) induced control, groups (3, 4, and 5) fed a diet with RGPS at different levels, 5, 10, and 20%, respectively. At the end of the feeding period, animals' blood and tissues were collected for estimation of serum lipid profile, serum liver, and kidney biomarkers. The protection was measured by detecting lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), Catalase (CAT) (in liver tissues), and liver histological examination. RESULTS: The results showed a significant (P < 0.05) decrease in levels of serum cholesterol, triglycerides, low density lipoprotein (LDL-C), and very low density lipoprotein (VLDL-C), with a significant increase in level of high density lipoprotein (HDL-C) for RGDS groups compared to induced control. Rats administered a diet containing RGDS levels produced significant (P < 0.05) hepatoprotection by decreasing the activities of liver enzymes, kidney parameters, and lipid peroxidation, while levels of GSH, SOD, and CAT were increased significantly to near the normal levels. CONCLUSION: The RGDS 20% group was more effective than others against hepatotoxicity of paracetamol, which may be attributed to RGDS total phenols and antioxidant contents, which were 1.438 mg and 1.231 mg, respectively.

The Effect of Antioxidant and Antimicrobial Activity on the Extracted Its Material and Aloe vera L. Callus Culture by the Natural Fruit Juice (천연과즙을 이용한 Aloe vera L.의 callus 배양과 이들 추출물의 항산화 및 항균활성 효과 검정)

  • Lee, In-Soon;Bae, Dong Nyeok;Kwon, Oh Min;Han, Gu Tai;Kim, Dae Hwan;Oh, Myeong Won;Lee, Ji Hong;Moon, Hae-Yeon
    • KSBB Journal
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    • v.28 no.6
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    • pp.408-414
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    • 2013
  • In order to obtain functional materials from aloe callus, we cultured Aloe vera L. leaf on MS medium added 0.2 mg/L IAA, 0.3 mg/L kinetin and 100 mg/L grape or/and apple juice for 30 days. While a callus differentiation during callus culture did not show, the cultured leaves were uniquely released extracellular material into the agar plate. After cultivation for 18 days, the cultured leaf and agar were harvested for extraction a functional material. The materials extracted were measured on the amount of total phenols, flavonoids and polysaccharides and determined on the antioxidant and antimicrobial activity. In result, callus extracts of additive free (CT) and added apple juice (2T) had more amount of phenol compound ($659{\mu}g/mL$, $533{\mu}g/mL$) and flavonoid ($580{\mu}g/mL$, $501{\mu}g/mL$) than natural leaf (p: $525{\mu}g/mL$, f: $301{\mu}g/mL$). However, the extract of natural leaf had the better effect of lipid peroxidation and polysaccharide content than the culture extract. All samples extracted had same effect on the nitrite scavenging activity. On the other hand, only 2T extract showed excellent 72% nitric oxide scavenging activity. The agar extract was also confirmed to contain polyphenol compound and polysaccharide content that had antioxidant and antimicrobial activity partly.

Effect of Burkholderia contaminans on Postharvest Diseases and Induced Resistance of Strawberry Fruits

  • Wang, Xiaoran;Shi, Junfeng;Wang, Rufu
    • The Plant Pathology Journal
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    • v.34 no.5
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    • pp.403-411
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    • 2018
  • This study takes strawberry-fruits as the test material and discusses the effect of Burkholderia contaminans B-1 on preventing postharvest diseases and inducing resistance-related substances in strawberry-fruits. Soaking and wound inoculating is performed to analyze the inhibitory effects of different treatment solutions on the gray mold of postharvest strawberry-fruits. The count of antagonistic bacteria colonies in the wound is found, and the dynamic growth of antagonistic bacteria and the pathogenic fungus is observed by electron microscopy. The results indicated that, either by soaking/wound-inoculating, the fermentation and suspension of antagonistic bacteria significantly reduced the incidence of postharvest diseases of strawberry-fruits. With wound inoculation, the inhibition rate of antagonist fermentation and suspension ($1{\times}10^{10}cfu/ml$) respectively reached 77.4% and 66.7%. It also led to a significant increase in the activity of resistance-related enzymes, i.e., phenylalanine ammonia lyase (PAL), 4-coumarate coenzyme A ligase (4CL), cinnamate-4-hydroxylase (C4H) and chalcone isomerase (CHI). On 1 d and 2 d post-treatment, the activity of 4CL was respectively 3.78 and 6.1 times of the control, and on 5 d, the activity of PAL was increased by 4.47 times the control. The treatment of antagonistic bacteria delayed the peaking of cinnamyl-alcohol dehydrogenase (CAD) activity and promoted the accumulation of lignin and total phenols. The antagonistic bacteria could be well colonized in the wounds. On 4-5 d post-inoculation, the count of colonies was $10^8$ times of that upon inoculation. Electronmicroscopy indicated that the antagonistic bacteria delayed the germination of pathogenic spores in the wounds, and inhibited further elongations of the mycelia.

Biochemical Reactions of Barley Leaves at Intervals After Inoculation with Erysiphe graminis f. sp. hordei (보리 흰가루병균 접종후 보리엽내 경시적 생화학반응)

  • Song Dong Up;Cho Baik Ho;Kim Ki Chung
    • Korean Journal Plant Pathology
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    • v.2 no.1
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    • pp.37-42
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    • 1986
  • Papilla and cytoplasmic aggregates clearly formed on the epidermal cells of barley leaves in response to the primary germ-tubes of Erysiphe graminis f. sp. hordei, but their sizes were much smaller than those in response to the appressoria. Some cells of barley leaves exposed to powdery mildew for 36-48h were more deeply stained as compared to the other cells by acid fuchsin. However, the content of malondialdehydein in powdery mildewed leaves, one of the product of lipid peroxidation, did not increase by 96h after inoculation. Positive reactions for callose, protein and phenolics were recognized in the papilla and cytoplasmic aggregates at 6h after inoculation, but cutin, suberin, cellulose and lignin were not noticeable until 72h after inoculation. The total phenol content in methanol extracts increased with increasing time after inoculation. All histochemical reactions were not race-specific in barley­powdery mildew combinations tested.

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A Test of Relative Removal Properties of Various Offensive Odors by Zeolite

  • Adelodun, Adedeji A.;Vellingiri, Kowsalya;Jeon, Byong-Hun;Oh, Jong-Min;Kumar, Sandeep;Kim, Ki-Hyun
    • Asian Journal of Atmospheric Environment
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    • v.11 no.1
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    • pp.15-28
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    • 2017
  • The adsorptive removal properties of synthetic A4 zeolite were investigated against a total of 16 offensive odors consisting of reduced sulfur compounds (RSCs), nitrogenous compounds (NCs), volatile fatty acids (VFAs), and phenols/indoles (PnI). Removal of these odors was measured using a laboratory-scale impinger-based adsorption setup containing 25 g of the zeolite bed (flow rate of $100mL\;min^{-1}$). The high est and lowest breakthrough (%) values were shown for PnIs and RSCs, respectively, and the maximum and minimum adsorption capacity (${\mu}g\;g^{-1}$) of the zeolite was observed for the RSCs (range of 0.77-3.4) and PnIs (0.06-0.104), respectively. As a result of sorptive removal by zeolite, a reduction in odor strength, measured as odor intensity (OI), was recorded from the minimum of approximately 0.7 OI units (indole [from 2.4 to 1.6]), skatole [2.2 to 1.4], and p-cresol [5.1 to 4.4]) to the maximum of approximately 4 OI units (methanethiol [11.4 to 7.5], n-valeric acid [10.4 to 6.5], i-butyric acid [7.9 to 4.4], and propionic acid [7.2 to 3.7]). Likewise, when removal was examined in terms of odor activity value (OAV), the extent of reduction was significant (i.e., 1000-fold) in the increasing order of amy acetate, i-butyric acid, phenol, propionic acid, and ammonia.

Volatile Flavor Components of Bangah (Agastache rugosa O. Kuntze) Herb (방아(Agastache rugosa O. Kuntze)의 향기성분)

  • Ahn, Bin;Yang, Cha-Bum
    • Korean Journal of Food Science and Technology
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    • v.23 no.5
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    • pp.582-586
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    • 1991
  • Bangah(Agastache rugosa O. Kuntze), grown in East Asia has very unique and strong flavor, known as wild plant adding to loach soup stock for deodorizing fishy in southern province Korea. In this work, flavor components in essential oil of bangah were analyzed by steam distillation/gaschromatography and identified by gas chromatography/mass selective detector. A total of 32 components were identified in essential oil including 14 hydrocarbons, 6 aldehydes, 5 phenols, 3 alcohol, 2 esters, 5 ketones and 1 other compounds. The major flavor components were estragole, limonene, caryophyllene, eugenol and anethol. It was newly appeared some of aldehydes and sesquiterpenes in ripened bangah. The flower portion was showed more various flavor profiles than leaf and stem. And the respective peak areas were also the largest in flower portion.

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