• Journal of Biosystems Engineering
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• v.27 no.4
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• pp.341-348
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• 2002

Quality evaluation of red ginsengs is determined by outer shape and inner qualities. Especially, the inner qualities are main grading criteria. Currently, red ginsengs are classified into 3-grades; heaven, earth and good. The best heaven grade must not include inner holes and sponge tissues. This study was conducted to develop a red ginseng sorting system using x-ray image processing technique. Because of lens characteristic, gray values of the central region in the x-ray image are higher and gradually decreased towards the edge regions. This difference of gray values gives trouble in segmentation and detection of inner holes in red ginseng image, so preprocessing technique is necessary. The preprocessing was done by subtracting source image from an empty background image. But, simple subtraction was not quite appropriate because of too small contrast between inner holes and sound part. Scaled subtraction images were obtained by multiplying all gray values by some numbers. However this method could not help to set threshold value because the gray values of root part are generally lower than body part when red ginseng is exposed to the x-ray. To determine threshold value for detecting inner holes, an algorithm was developed by increasing overall gray values of less clear images.

• Applied Microscopy
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• v.42 no.2
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• pp.105-109
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• 2012

Electron tomography (ET) of biological specimens is performed from a series of images obtained over a range of tilt angles in a transmission electron microscope. When using the high voltage electron microscope (HVEM), various noises appear in EM images acquired from thick sections by high voltage electron beam. In order to obtain an adequate result in electron tomograms that allow visualization of rather complex and mega-cellular structure such as brain tissue, it is necessary to remove the noise in each original tilt images of thick section. Using band-pass filtering of original tilt images, the filtered images are obtained and used to assemble a reconstructed tomogram. The qualified 3D tomogram from filtered images results in a considerable reduction of the noises compared to conventional tomogram. In conclusion, this study suggests that band-pass filtering is effective to improve the brightness and intensity of HVEM produced tomograms acquired from micron-thick sections of biological specimens.

• Journal of Food Hygiene and Safety
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• v.18 no.3
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• pp.161-165
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• 2003

The effects of Saururus chinensis Baill administration on the biochemical parameters of function in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated rats were investigated. Saururus chinensis Baill (200mg/kg) was administered into rats intraperitoneally for four weeks, seven days after the injection of TCDD(1 ${\mu}g/kg$). We examined the antioxidative enzymatic activity by measuring the level of AST and ALT in serum and SOD, Catalase, GPx, GSH and GSSG in liver tissue of rats. STT group showed 70.7% of inhibitive effect in AST acrivity compared to TTA group. ALT level of STT group was decreased to the level of NTT group. SOD and Catalase in TTA group were lower than in NTT group, but SOD and Catalase in STT group were increased by 82% and 55.45% respectively compared to TTA group. GSH contents in STT group were 74.20% increased compared to TTA group. GSSG contents in STT group were 61.08% decreased compared to TTA group. These results suggest that Saururus Chinensis Baill has a potent hepathprotective effect against TCDD intoxicated rats.

• Journal of Food Hygiene and Safety
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• v.18 no.3
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• pp.166-170
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• 2003

This study was carried out to investigate the inhibitive effect of Saururus chinenesis Baill (SCB) on lipid peroxidation in Sprague-Dawely rat (SD-rat) accutely exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Saururus chinenesis Baill (200 mg/kg) was administered into rats intraperitoneally for four weeks, seven days after the injection of TCDD (1 ${\mu}$g/kg). we examined th lipid parameters by measuring the levels of Total Cholestero, Triglyceride(TG), HDL-Cholestero, and LDL-Cholesterol in serum and Malone Dialdehyde (MDA) in liver tissue of rats. Cholesterol was siginificantly elevated in TCDD-treated abnormal group (TTA). The higher level of HDL-Cholesterol was found in Saururus chinenesis Baill and TCDD administered group (STT), which showed the lower levels of Total-Cholesterol and LDL-Cholesterol. TG content in the TTA was 34.84% increased compared to Non TCDD-treated group (NTT). That of STT was inhibited by 18.90% compared to TTA. MDA content in the TTA was 44.72% increased compared to NTT. That of STT was inhibited by 17.14% compared to TTA.

• Korean Journal of Acupuncture
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• v.26 no.3
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• pp.43-54
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• 2009

Objectives : To investigate the anti-inflammatory effects of Prunella vulgaris pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 5 groups; normal control (n=8), LPS control (n=8), LPS+Prunella vulgaris pharmacopuncture at CV4 (CV4, n=8), LPS+Prunella vulgaris pharmacopuncture at ST36 (ST36, n=8), and LPS+Prunella vulgaris pharmacopuncture at CV12 (CV12, n=8). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5mg/kg). Proinflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-10 (IL-10), thiobarbituric acid reactive substance (TBARS) from blood and liver tissue were compared before and 5 hrs after inflammation induction. Results : In CV4 and CV12 groups, plasma IL-$1{\beta}$, IL-6 and TNF-$\alpha$ levels increased by LPS injection, significantly decreased 5 hrs after injection (p<0.05). For CV12 group, plasma IL-10 concentration significantly increased (p<0.05). Liver IL-$1{\beta}$ and IL-6 levles significantly decreased in CV4 and CV12 groups (P<0.05), while normal and LPS control groups were not significantly different in TNF-$\alpha$ and IL-10 levels. Plasma TBARS concentration was significantly decreased in CV12 group, while there was no significant difference among LPS control and pharmacopuncture groups for liver TBARS concentration. Conclusions : Based on the present findings, Prunella vulgaris pharmacopuncture at CV12 may have a potentially preventive anti-inflammatory effect in an LPS-induced inflammatory rat model.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.1
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• pp.19-27
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• 2008

Thirty-six sinus grafts were performed in 34 patients with an alveolar crest bone height in the posterior maxilla of 3 to 5 mm before grafting. The sinuses were grafted using Bio-Oss alone or mixed with fibrin glue. Group 1 was the control group and included 25 patients who received a xenograft mixed in saline. Group 2 comprised 9 patients who received a xenograft and fibrin glue. The study was further subdivided at the time of 9 months. This histologic study evaluated by hematoxylin-eosin (H&E) and histomorphometric analysis whether fibrin glue in combination with Bio-Oss enhances bone regeneration in sinus floor elevation in humans. The new bone formation was better in Group 2 than in Group 1, but the difference was not significant. The absorption of the graft material was faster in Group 2 than in Group 1, in the short term, but better in Group 1 over the long term, although the difference was not significant. Lamellar bone was formed earlier in Group 1 compared to Group 2, but the difference was not significant. Overall, the surgery site stabilized earlier with new bone formation in Group 2 than in Group 1, but the difference was not significant. Combining a fibrin sealant and Bio-Oss could lead to improved scaffolds for bone tissue engineering based on the synergistic effects of the biomaterials. Therefore, Bio-Oss or Bio-Oss plus Tisseel may be used depending on the situation.

• Journal of Dental Rehabilitation and Applied Science
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• v.25 no.2
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• pp.191-200
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• 2009

Dental Pulp Stem Cells are superior to other types of adult stem cell. Because of teeth are easy to access and are extracted throughout life. A supernumerary tooth is an important clinical problem found in various populations of the world. The incidence of supernumerary teeth varies depending on the literature source. Pediatric dentists are routinely extracted them. However, no studies have been reported regarding Dental Pulp stem cells in a supernumerary tooth, and we failed to note that a valuable source of human stem cell. Herein, we tried to show that a supernumerary tooth contains cells that display the characteristic features of stem cells.

• The Journal of the Korean dental association
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• v.53 no.12
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• pp.935-948
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• 2015

목적: 법랑기질 유도체(EMD)가 사람 치주인대 줄기세포(hPDLSC)의 조직 형성능에 미치는 영향을 in vitro와 in vivo 분석 모델을 이용해 평가한다. 재료 및 방법: hPDLSC를 배양하여 운반체와 함께 면역 억제된 쥐 등에 이식하였다; (1)대조군: EMD 처치하지 않은 운반체에 심어진 hPDLSC군 ($EMD^-/hPDLSC^+$), (2)실험군: EMD 처치한 운반체에 심어진 hPDLSC군 ($EMD^+/hPDLSC^+$). 각 군당 5마리씩 시행하고 8주 후 희생하였다. 조직학적, 조직계측학적 분석을 통해 형성된 백악질의 면적과 백악세포의 수 그리고 샤피 섬유의 수를 계측하였으며 면역조직화학적 분석을 통해 백악질과 교원질 형성을 평가하였다. 또한 in vitro에서 hPDLSC의 수용성 교원질과 glycosaminoglycan 형성에 대한 EMD의 효과를 분석하였다. 결과: 조직학적 분석에서 교원질성 치주 인대 조직이 실험군에서 현저하게 많이 생성된 것을 관찰할 수 있었다. 형성된 백악질의 면적과 백악세포의 수는 군 간 차이가 없었으나, 새롭게 형성된 샤피 섬유의 수는 실험군에서 대조군보다 유의하게 많았다(p<0.05). 교원질 형성에 대한 면역조직 화학적 분석 결과, 실험군에서 I, III형 교원질과 hydroxyproline의 발현이 높았다. 또한 in vitro에서 hPDLSC에 의한 수용성 교원질과 glycosaminoglycan 형성이 EMD의 농도에 비례하여 증가하였다 (p<0.05). 결론: EMD는 hPDLSC에 의한 샤피 섬유 및 교원질 생성을 증가시키고, 이는 새로운 백악질의 기능적 부착과 치주조직 재생에 중요한 역할을 한다.

• The korean journal of orthodontics
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• v.45 no.6
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• pp.299-307
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• 2015

Objective: The aims of this study were to compare the shear bond strength between orthodontic metal brackets and glazed zirconia using different types of primer before applying resin cement and to determine which primer was more effective. Methods: Zirconia blocks were milled and embedded in acrylic resin and randomly assigned to one of four groups: nonglazed zirconia with sandblasting and zirconia primer (NZ); glazed zirconia with sandblasting, etching, and zirconia primer (GZ); glazed zirconia with sandblasting, etching, and porcelain primer (GP); and glazed zirconia with sandblasting, etching, zirconia primer, and porcelain primer (GZP). A stainless steel metal bracket was bonded to each target surface with resin cement, and all specimens underwent thermal cycling. The shear bond strength of the specimens was measured by a universal testing machine. A scanning electron microscope, three-dimensional optical surface-profiler, and stereoscopic microscope were used to image the zirconia surfaces. The data were analyzed with one-way analyses of variance and the Fisher exact test. Results: Group GZ showed significantly lower shear bond strength than did the other groups. No statistically significant differences were found among groups NZ, GP, and GZP. All specimens in group GZ showed adhesive failure between the zirconia and resin cement. In groups NZ and GP, bonding failed at the interface between the resin cement and bracket base or showed complex adhesive and cohesive failure. Conclusions: Porcelain primer is the more appropriate choice for bonding a metal bracket to the surface of a full-contour glazed zirconia crown with resin cement.

• Journal of Veterinary Clinics
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• v.25 no.5
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• pp.370-378
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• 2008

The aim of this study was to investigate bioactivities of 50% Chitin - hydroxyapatite (Chitin-H) compound and 50% Chitosan - hydroxyapatite (Chitosan-H) compound in canine bone. Ten healthy mongrel dogs (1-5 years old, 1.7 - 6.9 kg) were used in this study. These compounds had been transplanted into bilateral femur separately, and then the changes of femur were observed through the examinations of hemato-biochemical profiles, radiology, and histological profiles for 42 days. After 3 weeks, expanded radiolucent changes were observed in both areas transplanted the compounds. After 6 weeks, the area transplanted the Chitin-H compound did not observe any changes of bony tissue, while the area inserted the Chitosan-H compound was observed changes of increasing bone formation. In histological examination, infiltrations of inflammatory cells and bone absorptions were observed at both transplanted sites. However an increasing of active osteogenesis was observed at the transplanted site with Chitosan-H compound. In conclusion, Chitosan-H compound had an function of active osteogenesis as compared with Chitin-H compound. From this study, it is indicated that Chitosan-H compound would be used in dogs with severe bone defect.