한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제25권5호
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  • Pages.370-378
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  • 2008
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

키틴 유도체와 하이드록시아페타이트 복합체가 개의 뼈에 미치는 영향

The Effects of Chitin Derivative and Hydroxyapatite Compound in Canine Bone

  • 이해범 (전북대학교 수의과대학 생체안전성연구소) ;
  • 신승호 (전북대학교 수의과대학 생체안전성연구소) ;
  • 김민수 (전북대학교 수의과대학 생체안전성연구소) ;
  • 이기창 (전북대학교 수의과대학 생체안전성연구소) ;
  • 정용식 (전북대학교 공과대학) ;
  • 김남수 (전북대학교 수의과대학 생체안전성연구소)
  • Lee, Hae-Beom (College of Veterinary Medicine, Second stage Brain Korea 21, Bio-Safety Research Institute, Chonbuk National University) ;
  • Shin, Seung-Ho (College of Veterinary Medicine, Second stage Brain Korea 21, Bio-Safety Research Institute, Chonbuk National University) ;
  • Kim, Min-Su (College of Veterinary Medicine, Second stage Brain Korea 21, Bio-Safety Research Institute, Chonbuk National University) ;
  • Lee, Ki-Chang (College of Veterinary Medicine, Second stage Brain Korea 21, Bio-Safety Research Institute, Chonbuk National University) ;
  • Chung, Yong-Sik (College of Engineering, Chonbuk National University) ;
  • Kim, Nam-Soo (College of Veterinary Medicine, Second stage Brain Korea 21, Bio-Safety Research Institute, Chonbuk National University)
  • 발행 : 2008.10.31
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초록

The aim of this study was to investigate bioactivities of 50% Chitin - hydroxyapatite (Chitin-H) compound and 50% Chitosan - hydroxyapatite (Chitosan-H) compound in canine bone. Ten healthy mongrel dogs (1-5 years old, 1.7 - 6.9 kg) were used in this study. These compounds had been transplanted into bilateral femur separately, and then the changes of femur were observed through the examinations of hemato-biochemical profiles, radiology, and histological profiles for 42 days. After 3 weeks, expanded radiolucent changes were observed in both areas transplanted the compounds. After 6 weeks, the area transplanted the Chitin-H compound did not observe any changes of bony tissue, while the area inserted the Chitosan-H compound was observed changes of increasing bone formation. In histological examination, infiltrations of inflammatory cells and bone absorptions were observed at both transplanted sites. However an increasing of active osteogenesis was observed at the transplanted site with Chitosan-H compound. In conclusion, Chitosan-H compound had an function of active osteogenesis as compared with Chitin-H compound. From this study, it is indicated that Chitosan-H compound would be used in dogs with severe bone defect.

키워드

참고문헌

  1. D.R. Khanal, P. Choontanom, Y. Okamoto, S. Minami, S.k. Rakshit, S. Chandrakrachang, W.F. Stevens. Management of fracture with chitosan in dogs. Indian Vet. J. 2000; 77: 1085-1089
  2. D.R. Khanal, Y. Okamoto, K. Miyatake, T. Shinobu, Y. Shigemasa, S. Tokura, S.Minami. Protective effects of phosphated chitin (P-chitin) in a mice model of acute respiratory distress syndrome (ARDS). Carbohydr. Polym. 2001; 44: 99-106
  3. Ge Z, Baguenard S, Lim LY, Wee A, Khor E. Hydroxyapatitechitin materials as potential tissue engineered bone substitutes. Biomaterials. 2004; 25: 1049-1458 https://doi.org/10.1016/S0142-9612(03)00612-4
  4. H. Ueno, H. Yamada, I. Tanaka, N. Kaba, M. Matsuura, M. Okumura, T. Kadosawa, T. Fujinaga. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Boimaterials. 1999; 10: 1407-1414
  5. Huang YC, Vieira A, Huang KL, Yeh MK, Chiang CH. Pulmonary inflammation caused by chitosan microparticles. J Biomed Mater Res A. 2005; 75: 283-287
  6. Hwang SM, Chen CY, Chen SS, Chen JC. chtinous materials inhibit nitric oxide production by activated RAW 264.7 macrophages. Biochem Biophys Res Commun. 2000; 271: 229-233 https://doi.org/10.1006/bbrc.2000.2602
  7. Kofuji K, Akamine H, Qian CJ, Watanabe K, Togan Y, Nishimura M, Sugiyama I, Murata Y, Kawashima S. Therapeutic efficacy of sustained drug release from chitosan gel on local inflammation. Int J Pharm. 2004; 272:65-78 https://doi.org/10.1016/j.ijpharm.2003.11.036
  8. Kong L, Gao Y, Lu Guangyuan, Gong Y, Zhao N, Zhang X. A study on the bioactivity ofchitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering. Eur Polym J. 2006; 20.1016/j.eurpolymj.2006.08.009
  9. Lee JY, Kim KH, Shin SY, Rhyu IC, Lee YM, Park YJ, Chung CP, Lee SJ. Enhanced bone formation by transforming growth factor-beta1-releasing collagen/chitosan microgranules. J Biomed Mater Res A. 2006; 76: 530-539
  10. Minami, Y. Okamoto, K. Hamada, Y. Fukumoto, Y. Shigemasa, Veterinary practice with chitin and chitosan. in: P. Jolles, R.A.A. Muzzarelli (Eds), chtin and chitinases,. Birkhauser Verlag, Base, Switzerland, 1999; 265-277
  11. Mukherjee. D.P, Tunkle, A.S, Roberts R. A, Clavenna.S, Rongers. S, Smith.D. An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. J Biomed Mater Res B Appl Biomater. 2003; 67: 603-609
  12. Park YJ, Kim KH, Lee JY, Ku Y, Lee SJ, Min BM, Chung CP. Immobilization of bone morphogenetic protein-2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration. Biotechnol Appl Biochem. 2006; 43: 17-24 https://doi.org/10.1042/BA20050075
  13. Porporatto C, Bianco ID, Riera CM, Correa SG. chitosan induces different L-arginine metabolic pathways in resting and inflammatory macrophages. Biochem Biophys Res Commun. 2003; 304: 266-272 https://doi.org/10.1016/S0006-291X(03)00579-5
  14. R.A.A. Muzzarelli, V. Ramos, V. Stanic, B. Dubini, M. Mattioli-Belmonte, G. Tosi, R. Giardino. Osteogenesis promoted by calcium phosphate N, N-dicarboxymethyl chitosan. Carbohydr. Polym. 1998 36: 267-276 https://doi.org/10.1016/S0144-8617(98)00008-3
  15. Senel S, McClure SJ. Potential applicationsof chitosan in veterinary medicine. Adv Drug Deliv Rev. 2004; 56: 1467-1480 https://doi.org/10.1016/j.addr.2004.02.007
  16. Seol YJ, Lee JY, Park YJ, Lee YM, Young-Ku, Rhyu IC, Lee SJ, Han SB, Chung CP. chitosan sponges as tissue engineering scaffolds for bone formation. Biotechnol Lett. 2004; 26: 1037-1041 https://doi.org/10.1023/B:BILE.0000032962.79531.fd
  17. Uchida A, Araki N, Shinto Y, Yoshikawa H, Kurisaki E, Ono K. The use of calcium hydroxyapatite ceramic in bone tumour surgery. J Bone Joint Surg Br. 1990; 72: 298-302
  18. Ueno H, Murakami M, Okumura M, Kadosawa T, Uede T, Fujinaga T. Chitosan accelerates the production of osteopontin from polymorphonuclear leukocytes. Biomaterials. 2001 22:1667-1673 https://doi.org/10.1016/S0142-9612(00)00328-8
  19. Wang X, Ma J, Wang Y, He B. Structural characterization of phosphorylated chitosan and their applications as effective additives of calcium phosphate cements. Biomaterials. 2001; 22: 2247-2255 https://doi.org/10.1016/S0142-9612(00)00413-0
  20. Y. Okamoto, K. Shibazaki, S. Minami, A. Matsuhashi, S. Tanioka, Y. Shigemasa. Evaluation of chitin and chitosan on open wound healing in dogs. J. Vet. Med. Sci. 1995; 57: 851-854 https://doi.org/10.1292/jvms.57.851
  21. Y. Okamoto, L. southwood, T.S. Stashak, R. W. Norrdin, A.W.Nelson, S. Minami, A. Matsuhashi, K. Kato, Y. Shigemasa, Effect of chitin on nonwoven fabric implant in tendon healing. Carbohydr. Polym. 1997; 33: 33-38 https://doi.org/10.1016/S0144-8617(97)00040-4
  22. Zigang Ge, Sophie Baguenard, Lee Yong Lim, Aileen Wee, Eugene Khor. Hydroxyapatite-chitin materials as potential tissue engineered bone substitutes. Biomaterials. 2004; 25: 1049- 1058 https://doi.org/10.1016/S0142-9612(03)00612-4